Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles
Objective: To evaluate the effects of Catalpa bignonioides fruit extract on the promotion of muscle growth and muscular capacity in vitro and in vivo. Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell proliferation was assessed us...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=2;spage=47;epage=54;aulast=Jeong |
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| author | Hoibin Jeong Dong-joo Lee Sung-Pil Kwon SeonJu Park Song-Rae Kim Seung Hyun Kim Jae-Il Park Deug-chan Lee Kyung-Min Choi WonWoo Lee Ji-Won Park Bohyun Yun Su-Hyeon Cho Kil-Nam Kim |
| author_facet | Hoibin Jeong Dong-joo Lee Sung-Pil Kwon SeonJu Park Song-Rae Kim Seung Hyun Kim Jae-Il Park Deug-chan Lee Kyung-Min Choi WonWoo Lee Ji-Won Park Bohyun Yun Su-Hyeon Cho Kil-Nam Kim |
| author_sort | Hoibin Jeong |
| collection | DOAJ |
| description | Objective: To evaluate the effects of Catalpa bignonioides fruit extract on the promotion of muscle growth and muscular capacity in vitro and in vivo.
Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell proliferation was assessed using a 5-bromo-2’-deoxyuridine (BrdU) assay kit. Western blot analysis was performed to determine the protein expressions of related factors. The effects of Catalpa bignonioides extract were investigated in mice using the treadmill exhaustion test and whole-limb grip strength assay. Chemical composition analysis was performed using high-performance liquid chromatography (HPLC).
Results: Catalpa bignonioides extract increased the proliferation of C2C12 mouse myoblasts by activating the Akt/mTOR signaling pathway. It also induced metabolic changes, increasing the number of mitochondria and glucose metabolism by phosphorylating adenosine monophosphate-activated protein kinase. In an in vivo study, the extract-treated mice showed improved motor abilities, such as muscular endurance and grip strength. Additionally, HPLC analysis showed that vanillic acid may be the main component of the Catalpa bignonioides extract that enhanced muscle strength.
Conclusions: Catalpa bignonioides improves exercise performance through regulation of growth and metabolism in skeletal muscles, suggesting its potential as an effective natural agent for improving muscular strength. |
| first_indexed | 2024-04-24T19:42:53Z |
| format | Article |
| id | doaj.art-41feee798c0f4781840fea7651ccaae6 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-04-24T19:42:53Z |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-41feee798c0f4781840fea7651ccaae62024-03-25T10:07:27ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222024-01-01142475410.4103/apjtb.apjtb_779_23Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal musclesHoibin JeongDong-joo LeeSung-Pil KwonSeonJu ParkSong-Rae KimSeung Hyun KimJae-Il ParkDeug-chan LeeKyung-Min ChoiWonWoo LeeJi-Won ParkBohyun YunSu-Hyeon ChoKil-Nam KimObjective: To evaluate the effects of Catalpa bignonioides fruit extract on the promotion of muscle growth and muscular capacity in vitro and in vivo. Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell proliferation was assessed using a 5-bromo-2’-deoxyuridine (BrdU) assay kit. Western blot analysis was performed to determine the protein expressions of related factors. The effects of Catalpa bignonioides extract were investigated in mice using the treadmill exhaustion test and whole-limb grip strength assay. Chemical composition analysis was performed using high-performance liquid chromatography (HPLC). Results: Catalpa bignonioides extract increased the proliferation of C2C12 mouse myoblasts by activating the Akt/mTOR signaling pathway. It also induced metabolic changes, increasing the number of mitochondria and glucose metabolism by phosphorylating adenosine monophosphate-activated protein kinase. In an in vivo study, the extract-treated mice showed improved motor abilities, such as muscular endurance and grip strength. Additionally, HPLC analysis showed that vanillic acid may be the main component of the Catalpa bignonioides extract that enhanced muscle strength. Conclusions: Catalpa bignonioides improves exercise performance through regulation of growth and metabolism in skeletal muscles, suggesting its potential as an effective natural agent for improving muscular strength.http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=2;spage=47;epage=54;aulast=Jeongcatalpa bignonioides; skeletal muscle; cell proliferation; mitochondria; energy metabolism; c2c12 |
| spellingShingle | Hoibin Jeong Dong-joo Lee Sung-Pil Kwon SeonJu Park Song-Rae Kim Seung Hyun Kim Jae-Il Park Deug-chan Lee Kyung-Min Choi WonWoo Lee Ji-Won Park Bohyun Yun Su-Hyeon Cho Kil-Nam Kim Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles Asian Pacific Journal of Tropical Biomedicine catalpa bignonioides; skeletal muscle; cell proliferation; mitochondria; energy metabolism; c2c12 |
| title | Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles |
| title_full | Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles |
| title_fullStr | Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles |
| title_full_unstemmed | Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles |
| title_short | Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles |
| title_sort | catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles |
| topic | catalpa bignonioides; skeletal muscle; cell proliferation; mitochondria; energy metabolism; c2c12 |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=2;spage=47;epage=54;aulast=Jeong |
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