Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i>
Previous research has indicated that <i>Pseudomonas aurantiaca</i> ST-TJ4 possesses a notable antagonistic impact on <i>Phytophthora cinnamomi</i> and holds promising potential for biocontrol. In this study, a combination of a single-factor experiment, a Plackett–Burman desig...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/1/21 |
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| author | Wei-Liang Kong Yu Zhang Xiao-Qin Wu |
| author_facet | Wei-Liang Kong Yu Zhang Xiao-Qin Wu |
| author_sort | Wei-Liang Kong |
| collection | DOAJ |
| description | Previous research has indicated that <i>Pseudomonas aurantiaca</i> ST-TJ4 possesses a notable antagonistic impact on <i>Phytophthora cinnamomi</i> and holds promising potential for biocontrol. In this study, a combination of a single-factor experiment, a Plackett–Burman design and a response surface approach was employed to investigate the optimal formula of ST-TJ4 fermentation medium. Furthermore, the stability of ST-TJ4 fermentation filtrate and its biocontrol effect on <i>Ph. cinnamomi</i> in vivo were also evaluated. The results revealed that the optimal culture conditions for ST-TJ4 involved the use of 20.59 g/L of glucose and 18.76 g/L of yeast extract powder. Following optimization, the fermentation filtrate of ST-TJ4 exhibited an inhibition rate of 76.5%, representing a 15% increase compared to previous levels. Additionally, <i>phzA</i>, <i>phzB</i>, <i>phzD</i>, <i>phzE</i>, <i>phzF</i> and <i>phzO</i> genes involved in the synthesis of phenazine-1-carboxylic acid (PCA) and 2-hydroxyphenazine (2-OH-PHZ) were also upregulated. The ST-TJ4 fermentation filtrate demonstrated strong alkali resistance, weak acid resistance and favorable temperature and UV light stability. Furthermore, in vitro inoculation experiments confirmed that optimizing the fermentation medium reduced <i>Ps. cinnamomi</i>’s ability to infect the leaves of <i>Rhododendron pulchrum</i>. |
| first_indexed | 2024-03-08T10:57:06Z |
| format | Article |
| id | doaj.art-41ff96b6c24c47408dd1169cfa20e733 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-08T10:57:06Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-41ff96b6c24c47408dd1169cfa20e7332024-01-26T16:23:41ZengMDPI AGFermentation2311-56372023-12-011012110.3390/fermentation10010021Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i>Wei-Liang Kong0Yu Zhang1Xiao-Qin Wu2Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grassland, College of Soil and Water Conservation, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grassland, College of Soil and Water Conservation, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grassland, College of Soil and Water Conservation, Nanjing Forestry University, Nanjing 210037, ChinaPrevious research has indicated that <i>Pseudomonas aurantiaca</i> ST-TJ4 possesses a notable antagonistic impact on <i>Phytophthora cinnamomi</i> and holds promising potential for biocontrol. In this study, a combination of a single-factor experiment, a Plackett–Burman design and a response surface approach was employed to investigate the optimal formula of ST-TJ4 fermentation medium. Furthermore, the stability of ST-TJ4 fermentation filtrate and its biocontrol effect on <i>Ph. cinnamomi</i> in vivo were also evaluated. The results revealed that the optimal culture conditions for ST-TJ4 involved the use of 20.59 g/L of glucose and 18.76 g/L of yeast extract powder. Following optimization, the fermentation filtrate of ST-TJ4 exhibited an inhibition rate of 76.5%, representing a 15% increase compared to previous levels. Additionally, <i>phzA</i>, <i>phzB</i>, <i>phzD</i>, <i>phzE</i>, <i>phzF</i> and <i>phzO</i> genes involved in the synthesis of phenazine-1-carboxylic acid (PCA) and 2-hydroxyphenazine (2-OH-PHZ) were also upregulated. The ST-TJ4 fermentation filtrate demonstrated strong alkali resistance, weak acid resistance and favorable temperature and UV light stability. Furthermore, in vitro inoculation experiments confirmed that optimizing the fermentation medium reduced <i>Ps. cinnamomi</i>’s ability to infect the leaves of <i>Rhododendron pulchrum</i>.https://www.mdpi.com/2311-5637/10/1/21<i>Pseudomonas aurantiaca</i>fermentation medium<i>Phytophthora cinnamomi</i>biocontrol effect |
| spellingShingle | Wei-Liang Kong Yu Zhang Xiao-Qin Wu Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i> Fermentation <i>Pseudomonas aurantiaca</i> fermentation medium <i>Phytophthora cinnamomi</i> biocontrol effect |
| title | Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i> |
| title_full | Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i> |
| title_fullStr | Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i> |
| title_full_unstemmed | Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i> |
| title_short | Optimization of <i>Pseudomonas aurantiaca</i> ST-TJ4 Fermentation Medium and Its Control Effect on <i>Phytophthora cinnamomi</i> |
| title_sort | optimization of i pseudomonas aurantiaca i st tj4 fermentation medium and its control effect on i phytophthora cinnamomi i |
| topic | <i>Pseudomonas aurantiaca</i> fermentation medium <i>Phytophthora cinnamomi</i> biocontrol effect |
| url | https://www.mdpi.com/2311-5637/10/1/21 |
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