Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy
Background. In the pathogenesis of ischemic cardiomyopathy (ICMP), angiopoiesis remains unexplored.The aim. To describe the vasculature of the heart and the imbalance of angiogenesis mediators in the coronary circulation in association with the number of endothelial progenitor cells (EPC) and desqua...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2024-01-01
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| Series: | Acta Biomedica Scientifica |
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| Online Access: | https://www.actabiomedica.ru/jour/article/view/4494 |
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| author | S. P. Chumakova O. I. Urazova V. M. Shipulin I. V. Sukhodolo A. I. Stelmashenko O. A. Denisenko S. L. Andreev M. S. Demin E. G. Churina |
| author_facet | S. P. Chumakova O. I. Urazova V. M. Shipulin I. V. Sukhodolo A. I. Stelmashenko O. A. Denisenko S. L. Andreev M. S. Demin E. G. Churina |
| author_sort | S. P. Chumakova |
| collection | DOAJ |
| description | Background. In the pathogenesis of ischemic cardiomyopathy (ICMP), angiopoiesis remains unexplored.The aim. To describe the vasculature of the heart and the imbalance of angiogenesis mediators in the coronary circulation in association with the number of endothelial progenitor cells (EPC) and desquamated endothelial cells (DEC) in the blood of patients with coronary heart disease (CHD), suffering and not suffering from ICMP.Methods. Fifty-two patients with CHD (30 patients with ICMP, 22 patients without ICMP), 15 healthy donors were examined. The content of EPC (CD14+CD34+VEGFR2+) in the blood from the cubital vein and DEC (CD45–CD146+) in the blood from the coronary sinus and the cubital vein was determined by flow cytometry. The concentrations of VEGF-A (vascular endothelial growth factor A), PDGF (platelet-derived growth factor), and SDF-1 (stromal cell-derived factor 1) in blood plasma were recorded using immunofluorescence assay; the angiopoietin-2, MMP-9 (matrix metallopeptidase 9) were recorded using enzyme immunoassay. In myocardial biopsies the specific area of vessels and the expression of αSMA (smooth muscle alpha-actin) were determined by morphometric and immunohistochemical methods.Results. In the peripheral blood of patients with CHD, regardless of the presence of ICMP, the DEC content exceeded the physiological level, and the VEGF-A, PDGF, angiopoietin-2, and MMP-9 corresponded to the norm. In CHD patients without cardiomyopathy, there was an excess of SDF-1 and EPC in the blood from the cubital vein, and in ICMP, their physiological significance was noted. In the coronary blood flow in patients with CHD without cardiomyopathy, an increase in the concentration of PDGF was found, which was not determined in patients with ICMP, who had an increased content of DEC, angiopoietin-2 and MMP-9. The specific area of the vessels in the patients of the two groups was comparable; the expression of αSMA in ICMP was 6.2 times lower than in patients with CHD without cardiomyopathy.Conclusion. The development of ICMP is accompanied by impaired maturation of vessels in the myocardium, associated with the absence of a compensatory reaction of activation of cellular and humoral factors of angiogenesis. |
| first_indexed | 2024-03-08T12:14:49Z |
| format | Article |
| id | doaj.art-4204d4b23aba4e4d9d61dda54c0f69ab |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:14:45Z |
| publishDate | 2024-01-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-4204d4b23aba4e4d9d61dda54c0f69ab2024-03-28T07:49:29ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962024-01-0186819010.29413/ABS.2023-8.6.72658Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathyS. P. Chumakova0O. I. Urazova1V. M. Shipulin2I. V. Sukhodolo3A. I. Stelmashenko4O. A. Denisenko5S. L. Andreev6M. S. Demin7E. G. Churina8Siberian State Medical UniversitySiberian State Medical UniversityCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesSiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversityCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesSiberian State Medical UniversitySiberian State Medical University; National Research Tomsk State UniversityBackground. In the pathogenesis of ischemic cardiomyopathy (ICMP), angiopoiesis remains unexplored.The aim. To describe the vasculature of the heart and the imbalance of angiogenesis mediators in the coronary circulation in association with the number of endothelial progenitor cells (EPC) and desquamated endothelial cells (DEC) in the blood of patients with coronary heart disease (CHD), suffering and not suffering from ICMP.Methods. Fifty-two patients with CHD (30 patients with ICMP, 22 patients without ICMP), 15 healthy donors were examined. The content of EPC (CD14+CD34+VEGFR2+) in the blood from the cubital vein and DEC (CD45–CD146+) in the blood from the coronary sinus and the cubital vein was determined by flow cytometry. The concentrations of VEGF-A (vascular endothelial growth factor A), PDGF (platelet-derived growth factor), and SDF-1 (stromal cell-derived factor 1) in blood plasma were recorded using immunofluorescence assay; the angiopoietin-2, MMP-9 (matrix metallopeptidase 9) were recorded using enzyme immunoassay. In myocardial biopsies the specific area of vessels and the expression of αSMA (smooth muscle alpha-actin) were determined by morphometric and immunohistochemical methods.Results. In the peripheral blood of patients with CHD, regardless of the presence of ICMP, the DEC content exceeded the physiological level, and the VEGF-A, PDGF, angiopoietin-2, and MMP-9 corresponded to the norm. In CHD patients without cardiomyopathy, there was an excess of SDF-1 and EPC in the blood from the cubital vein, and in ICMP, their physiological significance was noted. In the coronary blood flow in patients with CHD without cardiomyopathy, an increase in the concentration of PDGF was found, which was not determined in patients with ICMP, who had an increased content of DEC, angiopoietin-2 and MMP-9. The specific area of the vessels in the patients of the two groups was comparable; the expression of αSMA in ICMP was 6.2 times lower than in patients with CHD without cardiomyopathy.Conclusion. The development of ICMP is accompanied by impaired maturation of vessels in the myocardium, associated with the absence of a compensatory reaction of activation of cellular and humoral factors of angiogenesis.https://www.actabiomedica.ru/jour/article/view/4494angiogenesisgrowth factorsendothelial progenitor cellsmyocardiumcoronary heart disease |
| spellingShingle | S. P. Chumakova O. I. Urazova V. M. Shipulin I. V. Sukhodolo A. I. Stelmashenko O. A. Denisenko S. L. Andreev M. S. Demin E. G. Churina Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy Acta Biomedica Scientifica angiogenesis growth factors endothelial progenitor cells myocardium coronary heart disease |
| title | Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy |
| title_full | Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy |
| title_fullStr | Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy |
| title_full_unstemmed | Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy |
| title_short | Production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy |
| title_sort | production of angiogenesis mediators and the structure of the vascular wall in the heart in ischemic cardiomyopathy |
| topic | angiogenesis growth factors endothelial progenitor cells myocardium coronary heart disease |
| url | https://www.actabiomedica.ru/jour/article/view/4494 |
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