Chromosomal assignment of canine <it>THADA </it>gene to CFA 10q25
<p>Abstract</p> <p>Background</p> <p>Chromosomal translocations affecting the chromosome 2p21 cluster in a 450 kb breakpoint region are frequently observed in human benign thyroid adenomas. <it>THADA </it>(thyroid adenoma associated) was identified as the af...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-06-01
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| Series: | Molecular Cytogenetics |
| Online Access: | http://www.molecularcytogenetics.org/content/1/1/11 |
| Summary: | <p>Abstract</p> <p>Background</p> <p>Chromosomal translocations affecting the chromosome 2p21 cluster in a 450 kb breakpoint region are frequently observed in human benign thyroid adenomas. <it>THADA </it>(thyroid adenoma associated) was identified as the affected gene within this breakpoint region. In contrast to man tumours of the thyroid gland of dogs (<it>Canis lupus familiaris</it>) constitute mainly as follicular cell carcinomas, with malignant thyroid tumours being more frequent than benign thyroid adenomas. In order to elucidate if the <it>THADA </it>gene is also a target of chromosomal rearrangements in thyroid adenomas of the dog we have physically mapped the canine <it>THADA </it>gene to canine chromosome 10.</p> <p>A PCR was established to screen a canine genome library for a BAC clone containing the gene sequence of canine <it>THADA</it>. Further PCR reactions were done using the identified BAC clone as a template in order to verify the corresponding PCR product by sequencing.</p> <p>Canine whole blood was incubated with colcemid in order to arrest the cultured cells in metaphases. The verified BAC DNA was digoxigenin labeled and used as a probe in fluorescence <it>in situ </it>hybridization (FISH). Ten well spread metaphases were examined indicating a signal on canine chromosome 10 on both chromatids. A detailed fine mapping was performed indicating the canine <it>THADA </it>gene locus on the q-arm of chromosome 10.</p> <p>Results</p> <p>The canine <it>THADA </it>gene locus was mapped on chromosome 10q25. Our mapping results obtained in this study following the previously described nomenclature for the canine karyotype.</p> <p>Conclusion</p> <p>We analysed whether the <it>THADA </it>gene locus is a hotspot of canine chromosomal rearrangements in canine neoplastic lesions of the thyroid and in addition might play a role as a candidate gene for a possible malignant transformation of canine thyroid adenomas. Although the available cytogenetic data of canine thyroid adenomas are still insufficient the chromosomal region to which the canine <it>THADA </it>has been mapped seems to be no hotspot of chromosomal aberrations seen in canine thyroid adenomas.</p> |
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| ISSN: | 1755-8166 |