Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis

Background: Protease-activated receptors (PAR-1 and PAR-2) are found to be immensely exhibited in the periodontal tissues. These receptors are involved in the inflammatory and repair processes. Activation of PARs is mainly by the bacterial and endogenous enzymes. The aim of the study was to determin...

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Main Authors: Sivasankari Thilagar, Muthukumar Santhanakrishnan, Suresh Rao
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2018-01-01
Series:Journal of Indian Society of Periodontology
Subjects:
Online Access:http://www.jisponline.com/article.asp?issn=0972-124X;year=2018;volume=22;issue=1;spage=12;epage=17;aulast=Thilagar
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author Sivasankari Thilagar
Muthukumar Santhanakrishnan
Suresh Rao
author_facet Sivasankari Thilagar
Muthukumar Santhanakrishnan
Suresh Rao
author_sort Sivasankari Thilagar
collection DOAJ
description Background: Protease-activated receptors (PAR-1 and PAR-2) are found to be immensely exhibited in the periodontal tissues. These receptors are involved in the inflammatory and repair processes. Activation of PARs is mainly by the bacterial and endogenous enzymes. The aim of the study was to determine the role of PAR-1 and PAR-2 in initiating periodontal inflammation and to immunolocalize these receptors in the gingival tissues of healthy and chronic periodontitis individuals. Materials and Methods: A total of 50 patients were selected for this study, of which 25 were healthy controls and 25 were chronic periodontitis patients. Gingival tissues were excised from the marginal gingiva and interdental papilla under local anesthesia (xylocaine with 2% adrenaline) during crown lengthening procedure or during periodontal therapy depending on the respective groups. Immunohistochemical analyses of PARs were done by staining the samples with hematoxylin and eosin and with primary and secondary antibody for PAR-1 and PAR-2. Results: The Hematoxylin and Eosin staining showed more inflammatory changes in the periodontitis group compared to healthy gingiva. In chronic periodontitis, PAR-1-positive cells were seen in the basal layer with a weak expression and were showing negative expression in the superficial layer. In consideration of PAR-2, there was a very strong expression up to the superficial layer of the epithelium, compared to PAR-1. On comparing the intensity of staining in the connective tissue of chronic periodontitis sample, there was an increased expression of PAR-2 compared with PAR-1. A low positive expression of PAR-1 and PAR-2 was observed in the epithelium and connective tissue of the healthy tissues. Conclusion: The results clearly demonstrated the role of PAR-1 and 2 in periodontal inflammation.
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spelling doaj.art-420abf89e3c04903830aac670642fbfd2022-12-21T18:43:06ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2018-01-01221121710.4103/jisp.jisp_287_17Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitisSivasankari ThilagarMuthukumar SanthanakrishnanSuresh RaoBackground: Protease-activated receptors (PAR-1 and PAR-2) are found to be immensely exhibited in the periodontal tissues. These receptors are involved in the inflammatory and repair processes. Activation of PARs is mainly by the bacterial and endogenous enzymes. The aim of the study was to determine the role of PAR-1 and PAR-2 in initiating periodontal inflammation and to immunolocalize these receptors in the gingival tissues of healthy and chronic periodontitis individuals. Materials and Methods: A total of 50 patients were selected for this study, of which 25 were healthy controls and 25 were chronic periodontitis patients. Gingival tissues were excised from the marginal gingiva and interdental papilla under local anesthesia (xylocaine with 2% adrenaline) during crown lengthening procedure or during periodontal therapy depending on the respective groups. Immunohistochemical analyses of PARs were done by staining the samples with hematoxylin and eosin and with primary and secondary antibody for PAR-1 and PAR-2. Results: The Hematoxylin and Eosin staining showed more inflammatory changes in the periodontitis group compared to healthy gingiva. In chronic periodontitis, PAR-1-positive cells were seen in the basal layer with a weak expression and were showing negative expression in the superficial layer. In consideration of PAR-2, there was a very strong expression up to the superficial layer of the epithelium, compared to PAR-1. On comparing the intensity of staining in the connective tissue of chronic periodontitis sample, there was an increased expression of PAR-2 compared with PAR-1. A low positive expression of PAR-1 and PAR-2 was observed in the epithelium and connective tissue of the healthy tissues. Conclusion: The results clearly demonstrated the role of PAR-1 and 2 in periodontal inflammation.http://www.jisponline.com/article.asp?issn=0972-124X;year=2018;volume=22;issue=1;spage=12;epage=17;aulast=ThilagarChronic periodontitisimmunityimmunohistochemistryinflammationPorphyromonas gingivalisprotease-activated receptor
spellingShingle Sivasankari Thilagar
Muthukumar Santhanakrishnan
Suresh Rao
Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis
Journal of Indian Society of Periodontology
Chronic periodontitis
immunity
immunohistochemistry
inflammation
Porphyromonas gingivalis
protease-activated receptor
title Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis
title_full Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis
title_fullStr Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis
title_full_unstemmed Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis
title_short Expression of protease-activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis
title_sort expression of protease activated receptors 1 and 2 in individuals with healthy gingiva and chronic periodontitis
topic Chronic periodontitis
immunity
immunohistochemistry
inflammation
Porphyromonas gingivalis
protease-activated receptor
url http://www.jisponline.com/article.asp?issn=0972-124X;year=2018;volume=22;issue=1;spage=12;epage=17;aulast=Thilagar
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