The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot study
Introduction: The testes are immune privileged organs but they are subjected to acute and chronic inflammations that can impair spermatogenesis. Spermatogonial stem cells are less sensitive to injury, capable of self-renewal, and act as a reservoir that can restart impaired spermatogenesis. Objectiv...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2016-03-01
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| Series: | Middle East Fertility Society Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569015300236 |
| _version_ | 1818319068909797376 |
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| author | Ahmed M Hassanin Essam Ayad |
| author_facet | Ahmed M Hassanin Essam Ayad |
| author_sort | Ahmed M Hassanin |
| collection | DOAJ |
| description | Introduction: The testes are immune privileged organs but they are subjected to acute and chronic inflammations that can impair spermatogenesis. Spermatogonial stem cells are less sensitive to injury, capable of self-renewal, and act as a reservoir that can restart impaired spermatogenesis. Objective: To study the association of chronic testicular inflammatory infiltration with an area of complete spermatogenesis in testicular biopsies of men with non-obstructive azoospermia (NOA). Design: Retrospective study. Patients and methods: Review of 109 archived Bouin fixed, paraffin embedded and hematoxylin and eosin stained open testicular biopsies of azoospermic men. Main outcome measure: The absence/presence (focal/widespread) of chronic interstitial inflammatory infiltration and absence/presence of late spermatids. Results: Obstructive picture was found in 25 (22.9%) whereas non-obstructive patterns were found in 84 (77.1%) of the reviewed biopsies. Focal interstitial lymphocytic infiltration (ILI) was found in 9 (36%) obstructive biopsies whereas in non-obstructive patterns 32 (38.1%) had focal and 27 (32.1%) had wide spread ILI. In NOA late spermatids were present in 32 (38.1%) biopsies. Finding late spermatids was significantly highest when the infiltrate was focal and significantly lowest when the infiltrate was wide spread. Conclusion: In NOA, the possibility of finding an area of complete focal spermatogenesis in the open testicular biopsy is significantly highest when there is a focal type of ILI, but the possibility is significantly lowest when the infiltrate is widespread. This may guide the clinical testicular sperm extraction (TESE) procedure. |
| first_indexed | 2024-12-13T10:03:15Z |
| format | Article |
| id | doaj.art-420b57e223924a66803cb2032c9738ac |
| institution | Directory Open Access Journal |
| issn | 1110-5690 |
| language | English |
| last_indexed | 2024-12-13T10:03:15Z |
| publishDate | 2016-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj.art-420b57e223924a66803cb2032c9738ac2022-12-21T23:51:35ZengSpringerOpenMiddle East Fertility Society Journal1110-56902016-03-01211313510.1016/j.mefs.2015.07.003The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot studyAhmed M Hassanin0Essam Ayad1Department of Andrology, Faculty of Medicine, Cairo University, Cairo, EgyptDepartment of Pathology, Faculty of Medicine, Cairo University, Cairo, EgyptIntroduction: The testes are immune privileged organs but they are subjected to acute and chronic inflammations that can impair spermatogenesis. Spermatogonial stem cells are less sensitive to injury, capable of self-renewal, and act as a reservoir that can restart impaired spermatogenesis. Objective: To study the association of chronic testicular inflammatory infiltration with an area of complete spermatogenesis in testicular biopsies of men with non-obstructive azoospermia (NOA). Design: Retrospective study. Patients and methods: Review of 109 archived Bouin fixed, paraffin embedded and hematoxylin and eosin stained open testicular biopsies of azoospermic men. Main outcome measure: The absence/presence (focal/widespread) of chronic interstitial inflammatory infiltration and absence/presence of late spermatids. Results: Obstructive picture was found in 25 (22.9%) whereas non-obstructive patterns were found in 84 (77.1%) of the reviewed biopsies. Focal interstitial lymphocytic infiltration (ILI) was found in 9 (36%) obstructive biopsies whereas in non-obstructive patterns 32 (38.1%) had focal and 27 (32.1%) had wide spread ILI. In NOA late spermatids were present in 32 (38.1%) biopsies. Finding late spermatids was significantly highest when the infiltrate was focal and significantly lowest when the infiltrate was wide spread. Conclusion: In NOA, the possibility of finding an area of complete focal spermatogenesis in the open testicular biopsy is significantly highest when there is a focal type of ILI, but the possibility is significantly lowest when the infiltrate is widespread. This may guide the clinical testicular sperm extraction (TESE) procedure.http://www.sciencedirect.com/science/article/pii/S1110569015300236SpermatogenesisLymphocytic inflammatory infiltrationTestisAzoospermia |
| spellingShingle | Ahmed M Hassanin Essam Ayad The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot study Middle East Fertility Society Journal Spermatogenesis Lymphocytic inflammatory infiltration Testis Azoospermia |
| title | The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot study |
| title_full | The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot study |
| title_fullStr | The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot study |
| title_full_unstemmed | The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot study |
| title_short | The impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men, evidence-based pilot study |
| title_sort | impact of chronic testicular inflammatory infiltration on spermatogenesis in azoospermic men evidence based pilot study |
| topic | Spermatogenesis Lymphocytic inflammatory infiltration Testis Azoospermia |
| url | http://www.sciencedirect.com/science/article/pii/S1110569015300236 |
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