Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV
Success of antiviral therapy for chronic hepatitis B is supported by highly sensitive PCR-based assays for Hepatitis B virus (HBV) DNA. Nucleic acid extraction from biologic specimens is technically demanding and reliable PCR results depend it. Performances of the fully automatic system COBAS AmpliP...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2007-12-01
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| Series: | Microbiologia Medica |
| Subjects: | |
| Online Access: | http://www.pagepressjournals.org/index.php/mm/article/view/2606 |
| _version_ | 1818754397892509696 |
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| author | Tiziano Allice Francesco Cerutti Fabrizia Pittaluga Silvia Varetto Silvia Gabella Alfredo Marzano Alessandro Franchello Valeria Ghisetti |
| author_facet | Tiziano Allice Francesco Cerutti Fabrizia Pittaluga Silvia Varetto Silvia Gabella Alfredo Marzano Alessandro Franchello Valeria Ghisetti |
| author_sort | Tiziano Allice |
| collection | DOAJ |
| description | Success of antiviral therapy for chronic hepatitis B is supported by highly sensitive PCR-based assays for Hepatitis B virus (HBV) DNA. Nucleic acid extraction from biologic specimens is technically demanding and reliable PCR results depend it. Performances of the fully automatic system COBAS AmpliPrep™/COBAS TaqMan™ 48 (CAP/CTM) (Roche, Branchburg, NJ) for HBV DNA extraction and real -time PCR quantification were assessed and compared with the end-point PCR COBAS AMPLICOR HBV Monitor (CAHBM, Roche). Analytical evaluation with a proficiency panel showed that CAP/CTM quantitated HBV DNA levels in one single run over a wide dynamic range (7 logs) with a close correlation between expected and observed values (r=0.976, interassay variability below 5%). Clinical evaluation as tested with samples from 92 HBsAg-positive patients, demonstrated excellent correlation with CAHBM (r=0.966, mean difference in quantitation: 0.36 log10 IU/ml). CAP/CTM detected 10% more viremic patients and longer period of residual viremia in those on therapy. In lamivudine (LAM)-resistant patients, reduction of HBV DNA after 12 months of Adefovir (ADF) was higher in the combination (LAM+ADF) schedule than in ADF monotherapy (5.1 vs. 3.5 logs) suggesting a benefit in continuing LAM. In conclusion,CAP/CTM can improve the management of HBV infection, the assessment of antiviral therapy and drug resistance, supporting further insights in the emerging area of drug resistance. |
| first_indexed | 2024-12-18T05:22:37Z |
| format | Article |
| id | doaj.art-420bdef824c04941a90e74c470dc1d7c |
| institution | Directory Open Access Journal |
| issn | 2280-6423 |
| language | English |
| last_indexed | 2024-12-18T05:22:37Z |
| publishDate | 2007-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Microbiologia Medica |
| spelling | doaj.art-420bdef824c04941a90e74c470dc1d7c2022-12-21T21:19:38ZengPAGEPress PublicationsMicrobiologia Medica2280-64232007-12-0122410.4081/mm.2007.26061859Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBVTiziano AlliceFrancesco CeruttiFabrizia PittalugaSilvia VarettoSilvia GabellaAlfredo MarzanoAlessandro FranchelloValeria GhisettiSuccess of antiviral therapy for chronic hepatitis B is supported by highly sensitive PCR-based assays for Hepatitis B virus (HBV) DNA. Nucleic acid extraction from biologic specimens is technically demanding and reliable PCR results depend it. Performances of the fully automatic system COBAS AmpliPrep™/COBAS TaqMan™ 48 (CAP/CTM) (Roche, Branchburg, NJ) for HBV DNA extraction and real -time PCR quantification were assessed and compared with the end-point PCR COBAS AMPLICOR HBV Monitor (CAHBM, Roche). Analytical evaluation with a proficiency panel showed that CAP/CTM quantitated HBV DNA levels in one single run over a wide dynamic range (7 logs) with a close correlation between expected and observed values (r=0.976, interassay variability below 5%). Clinical evaluation as tested with samples from 92 HBsAg-positive patients, demonstrated excellent correlation with CAHBM (r=0.966, mean difference in quantitation: 0.36 log10 IU/ml). CAP/CTM detected 10% more viremic patients and longer period of residual viremia in those on therapy. In lamivudine (LAM)-resistant patients, reduction of HBV DNA after 12 months of Adefovir (ADF) was higher in the combination (LAM+ADF) schedule than in ADF monotherapy (5.1 vs. 3.5 logs) suggesting a benefit in continuing LAM. In conclusion,CAP/CTM can improve the management of HBV infection, the assessment of antiviral therapy and drug resistance, supporting further insights in the emerging area of drug resistance.http://www.pagepressjournals.org/index.php/mm/article/view/2606HBV, Real-time PCR , quantification |
| spellingShingle | Tiziano Allice Francesco Cerutti Fabrizia Pittaluga Silvia Varetto Silvia Gabella Alfredo Marzano Alessandro Franchello Valeria Ghisetti Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV Microbiologia Medica HBV, Real-time PCR , quantification |
| title | Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV |
| title_full | Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV |
| title_fullStr | Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV |
| title_full_unstemmed | Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV |
| title_short | Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV |
| title_sort | real time pcr per hbv dna valutazione del nuovo sistema automatizzato cobas ampliprep™ cobas taqman™ hbv |
| topic | HBV, Real-time PCR , quantification |
| url | http://www.pagepressjournals.org/index.php/mm/article/view/2606 |
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