SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis
Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1027551/full |
| _version_ | 1828162501421826048 |
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| author | Xiaoyan Zou Xiaoyan Zou Rui Huang Linjie Wang Linjie Wang Guihua Wang Ye Miao Ye Miao Idupulapati Rao Guodao Liu Zhijian Chen Zhijian Chen |
| author_facet | Xiaoyan Zou Xiaoyan Zou Rui Huang Linjie Wang Linjie Wang Guihua Wang Ye Miao Ye Miao Idupulapati Rao Guodao Liu Zhijian Chen Zhijian Chen |
| author_sort | Xiaoyan Zou |
| collection | DOAJ |
| description | Transporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Δsmf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane–localized transporter responsible for Mn uptake in stylo. |
| first_indexed | 2024-04-12T00:56:32Z |
| format | Article |
| id | doaj.art-420c20a25c45400cb276ace22e8b21aa |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T00:56:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-420c20a25c45400cb276ace22e8b21aa2022-12-22T03:54:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10275511027551SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensisXiaoyan Zou0Xiaoyan Zou1Rui Huang2Linjie Wang3Linjie Wang4Guihua Wang5Ye Miao6Ye Miao7Idupulapati Rao8Guodao Liu9Zhijian Chen10Zhijian Chen11Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaKey Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaCrops for Nutrition and Health, Alliance of Bioversity International and International Center for Tropical Agriculture, Cali, ColombiaKey Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaTransporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Δsmf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane–localized transporter responsible for Mn uptake in stylo.https://www.frontiersin.org/articles/10.3389/fpls.2022.1027551/fullnramptrace metalmetal homeostasisgene expressionsubcellular localization |
| spellingShingle | Xiaoyan Zou Xiaoyan Zou Rui Huang Linjie Wang Linjie Wang Guihua Wang Ye Miao Ye Miao Idupulapati Rao Guodao Liu Zhijian Chen Zhijian Chen SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis Frontiers in Plant Science nramp trace metal metal homeostasis gene expression subcellular localization |
| title | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_full | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_fullStr | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_full_unstemmed | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_short | SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis |
| title_sort | sgnramp1 a plasma membrane localized transporter involves in manganese uptake in stylosanthes guianensis |
| topic | nramp trace metal metal homeostasis gene expression subcellular localization |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1027551/full |
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