Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health state
<p>Abstract</p> <p>Background</p> <p>The endemic seagrass <it>Posidonia oceanica </it>(L.) Delile colonizes soft bottoms producing highly productive meadows that play a crucial role in coastal ecosystems dynamics. Human activities and natural events are resp...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-07-01
|
| Series: | BMC Ecology |
| Online Access: | http://www.biomedcentral.com/1472-6785/7/6 |
| _version_ | 1798029232189734912 |
|---|---|
| author | Randazzo Davide Rotini Alice Migliore Luciana Albanese Nadia N Giallongo Agata |
| author_facet | Randazzo Davide Rotini Alice Migliore Luciana Albanese Nadia N Giallongo Agata |
| author_sort | Randazzo Davide |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The endemic seagrass <it>Posidonia oceanica </it>(L.) Delile colonizes soft bottoms producing highly productive meadows that play a crucial role in coastal ecosystems dynamics. Human activities and natural events are responsible for a widespread meadows regression; to date the identification of "diagnostic" tools to monitor conservation status is a critical issue. In this study the feasibility of a novel tool to evaluate ecological impacts on <it>Posidonia </it>meadows has been tested. Quantification of a putative stress indicator, <it>i.e</it>. phenols content, has been coupled to 2-D electrophoretic protein analysis of rhizome samples.</p> <p>Results</p> <p>The overall expression pattern from <it>Posidonia </it>rhizome was determined using a preliminary proteomic approach, 437 protein spots were characterized by p<it>I </it>and molecular weight. We found that protein expression differs in samples belonging to sites with high or low phenols: 22 unique protein spots are peculiar of "low phenols" and 27 other spots characterize "high phenols" samples.</p> <p>Conclusion</p> <p><it>Posidonia </it>showed phenols variations within the meadow, that probably reflect the heterogeneity of environmental pressures. In addition, comparison of the 2-D electrophoresis patterns allowed to highlight qualitative protein expression differences in response to these pressures. These differences may account for changes in metabolic/physiological pathways as adaptation to stress. A combined approach, based on phenols content determination and 2-D electrophoresis protein pattern, seems a promising tool to monitor <it>Posidonia </it>meadows health state.</p> |
| first_indexed | 2024-04-11T19:20:53Z |
| format | Article |
| id | doaj.art-420c2656bc1a456cba26a57aa7c00cd8 |
| institution | Directory Open Access Journal |
| issn | 1472-6785 |
| language | English |
| last_indexed | 2024-04-11T19:20:53Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ecology |
| spelling | doaj.art-420c2656bc1a456cba26a57aa7c00cd82022-12-22T04:07:18ZengBMCBMC Ecology1472-67852007-07-0171610.1186/1472-6785-7-6Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health stateRandazzo DavideRotini AliceMigliore LucianaAlbanese Nadia NGiallongo Agata<p>Abstract</p> <p>Background</p> <p>The endemic seagrass <it>Posidonia oceanica </it>(L.) Delile colonizes soft bottoms producing highly productive meadows that play a crucial role in coastal ecosystems dynamics. Human activities and natural events are responsible for a widespread meadows regression; to date the identification of "diagnostic" tools to monitor conservation status is a critical issue. In this study the feasibility of a novel tool to evaluate ecological impacts on <it>Posidonia </it>meadows has been tested. Quantification of a putative stress indicator, <it>i.e</it>. phenols content, has been coupled to 2-D electrophoretic protein analysis of rhizome samples.</p> <p>Results</p> <p>The overall expression pattern from <it>Posidonia </it>rhizome was determined using a preliminary proteomic approach, 437 protein spots were characterized by p<it>I </it>and molecular weight. We found that protein expression differs in samples belonging to sites with high or low phenols: 22 unique protein spots are peculiar of "low phenols" and 27 other spots characterize "high phenols" samples.</p> <p>Conclusion</p> <p><it>Posidonia </it>showed phenols variations within the meadow, that probably reflect the heterogeneity of environmental pressures. In addition, comparison of the 2-D electrophoresis patterns allowed to highlight qualitative protein expression differences in response to these pressures. These differences may account for changes in metabolic/physiological pathways as adaptation to stress. A combined approach, based on phenols content determination and 2-D electrophoresis protein pattern, seems a promising tool to monitor <it>Posidonia </it>meadows health state.</p>http://www.biomedcentral.com/1472-6785/7/6 |
| spellingShingle | Randazzo Davide Rotini Alice Migliore Luciana Albanese Nadia N Giallongo Agata Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health state BMC Ecology |
| title | Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health state |
| title_full | Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health state |
| title_fullStr | Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health state |
| title_full_unstemmed | Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health state |
| title_short | Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor <it>Posidonia </it>meadows health state |
| title_sort | phenols content and 2 d electrophoresis protein pattern a promising tool to monitor it posidonia it meadows health state |
| url | http://www.biomedcentral.com/1472-6785/7/6 |
| work_keys_str_mv | AT randazzodavide phenolscontentand2delectrophoresisproteinpatternapromisingtooltomonitoritposidoniaitmeadowshealthstate AT rotinialice phenolscontentand2delectrophoresisproteinpatternapromisingtooltomonitoritposidoniaitmeadowshealthstate AT miglioreluciana phenolscontentand2delectrophoresisproteinpatternapromisingtooltomonitoritposidoniaitmeadowshealthstate AT albanesenadian phenolscontentand2delectrophoresisproteinpatternapromisingtooltomonitoritposidoniaitmeadowshealthstate AT giallongoagata phenolscontentand2delectrophoresisproteinpatternapromisingtooltomonitoritposidoniaitmeadowshealthstate |