Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen Challenge
<p/> <p>Interleukin (IL)-9 is a pleiotropic T helper 2-type cytokine that has been shown to be up-regulated in allergic airway disease, including asthma. IL-9 has been demonstrated to be a potent stimulus for the production and secretion of mucus from airway epithelial cells via inductio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-03-01
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| Series: | Allergy, Asthma & Clinical Immunology |
| Online Access: | http://www.aacijournal.com/content/3/1/19 |
| _version_ | 1818789945911803904 |
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| author | Hauber Hans-Peter Bergeron Celine Toda Masao Kontolemos Mario Holroyd Kenneth J Levitt Roy C Hamid Qutayba |
| author_facet | Hauber Hans-Peter Bergeron Celine Toda Masao Kontolemos Mario Holroyd Kenneth J Levitt Roy C Hamid Qutayba |
| author_sort | Hauber Hans-Peter |
| collection | DOAJ |
| description | <p/> <p>Interleukin (IL)-9 is a pleiotropic T helper 2-type cytokine that has been shown to be up-regulated in allergic airway disease, including asthma. IL-9 has been demonstrated to be a potent stimulus for the production and secretion of mucus from airway epithelial cells via induction of a calcium-activated chloride channel, hCLCA1. The objective of this study was to investigate the expression of IL-9 and hCLCA1 following allergen challenge in the nasal mucosa of allergic rhinitis patients. Nasal biopsies were obtained from allergic rhinitis patients out of allergen season both before (baseline) and after local antigen challenge with either ragweed or diluent (control). Immunohistochemistry and in situ hybridization were used to assess IL-9 protein and hCLCA1 messenger ribonucleic acid. Eosinophils and T cells were detected using immunohistochemistry. IL-9 and hCLCA1 were very low at baseline, and expression was significantly up-regulated following ragweed challenge. Whereas the number of eosinophils increased after allergen challenge, T-cell counts did not change significantly. The results of this study demonstrate the relationship between specific allergen challenge and expression of both IL-9 and hCLCA1, suggesting a possible mechanism for the increased production of mucus from airway epithelial cells in allergic rhinitis.</p> |
| first_indexed | 2024-12-18T14:47:38Z |
| format | Article |
| id | doaj.art-420defafa7d5474db5ff6e79ab7927a7 |
| institution | Directory Open Access Journal |
| issn | 1710-1484 1710-1492 |
| language | English |
| last_indexed | 2024-12-18T14:47:38Z |
| publishDate | 2007-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Allergy, Asthma & Clinical Immunology |
| spelling | doaj.art-420defafa7d5474db5ff6e79ab7927a72022-12-21T21:04:15ZengBMCAllergy, Asthma & Clinical Immunology1710-14841710-14922007-03-0131192310.1186/1710-1492-3-1-19Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen ChallengeHauber Hans-PeterBergeron CelineToda MasaoKontolemos MarioHolroyd Kenneth JLevitt Roy CHamid Qutayba<p/> <p>Interleukin (IL)-9 is a pleiotropic T helper 2-type cytokine that has been shown to be up-regulated in allergic airway disease, including asthma. IL-9 has been demonstrated to be a potent stimulus for the production and secretion of mucus from airway epithelial cells via induction of a calcium-activated chloride channel, hCLCA1. The objective of this study was to investigate the expression of IL-9 and hCLCA1 following allergen challenge in the nasal mucosa of allergic rhinitis patients. Nasal biopsies were obtained from allergic rhinitis patients out of allergen season both before (baseline) and after local antigen challenge with either ragweed or diluent (control). Immunohistochemistry and in situ hybridization were used to assess IL-9 protein and hCLCA1 messenger ribonucleic acid. Eosinophils and T cells were detected using immunohistochemistry. IL-9 and hCLCA1 were very low at baseline, and expression was significantly up-regulated following ragweed challenge. Whereas the number of eosinophils increased after allergen challenge, T-cell counts did not change significantly. The results of this study demonstrate the relationship between specific allergen challenge and expression of both IL-9 and hCLCA1, suggesting a possible mechanism for the increased production of mucus from airway epithelial cells in allergic rhinitis.</p>http://www.aacijournal.com/content/3/1/19 |
| spellingShingle | Hauber Hans-Peter Bergeron Celine Toda Masao Kontolemos Mario Holroyd Kenneth J Levitt Roy C Hamid Qutayba Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen Challenge Allergy, Asthma & Clinical Immunology |
| title | Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen Challenge |
| title_full | Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen Challenge |
| title_fullStr | Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen Challenge |
| title_full_unstemmed | Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen Challenge |
| title_short | Up-Regulation of Interleukin-9 and the Interleukin-9-Associated Calcium-Activated Chloride Channel hCLCA1 in Nasal Mucosa Following In Vivo Allergen Challenge |
| title_sort | up regulation of interleukin 9 and the interleukin 9 associated calcium activated chloride channel hclca1 in nasal mucosa following in vivo allergen challenge |
| url | http://www.aacijournal.com/content/3/1/19 |
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