Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary Artery
Cardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes fro...
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MDPI AG
2020-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1688 |
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| author | Deepak Adhikari Dal-Seong Gong Se Hee Oh Eun Hee Sung Seung On Lee Dong-Wook Kim Min-Ho Oak Hyun Jung Kim |
| author_facet | Deepak Adhikari Dal-Seong Gong Se Hee Oh Eun Hee Sung Seung On Lee Dong-Wook Kim Min-Ho Oak Hyun Jung Kim |
| author_sort | Deepak Adhikari |
| collection | DOAJ |
| description | Cardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes from <i>Boesenbergia rotunda</i> (L.) Mansf. [<i>Boesenbergia pandurata</i> (Roxb.) Schltr.] used as a spice and herbal medicine in Asian countries. The methanol extract of <i>B. rotunda</i> rhizomes (BRE) exhibited significant vasorelaxation effects ex vivo at EC<sub>50</sub> values of 13.4 ± 6.1 μg/mL and 40.9 ± 7.9 μg/mL, respectively, with and without endothelium in the porcine coronary artery ring. The intrinsic mechanism was evaluated by treating with specific inhibitors or activators that typically affect vascular reactivity. The results suggested that BRE induced relaxation in the coronary artery rings via an endothelium-dependent pathway involving NO-cGMP, and also via an endothelium-independent pathway involving the blockade of Ca<sup>2+</sup> channels. Vasorelaxant principles in BRE were identified by subsequent chromatographic methods, which revealed that flavonoids regulate vasorelaxant activity in BRE. One of the flavonoids was a Diels-Alder type adduct, 4-hydroxypanduratin A, which showed the most potent vasorelaxant effect on porcine coronary artery with an EC<sub>50</sub> of 17.8 ± 2.5 μM. Our results suggest that rhizomes of <i>B. rotunda</i> might be of interest as herbal medicine against cardiovascular diseases. |
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| last_indexed | 2024-03-10T14:24:06Z |
| publishDate | 2020-12-01 |
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| series | Plants |
| spelling | doaj.art-420ec23344cc44049f83fed9a78a91d52023-11-20T23:06:23ZengMDPI AGPlants2223-77472020-12-01912168810.3390/plants9121688Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary ArteryDeepak Adhikari0Dal-Seong Gong1Se Hee Oh2Eun Hee Sung3Seung On Lee4Dong-Wook Kim5Min-Ho Oak6Hyun Jung Kim7College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-gun 58554, KoreaCollege of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-gun 58554, KoreaCollege of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-gun 58554, KoreaCollege of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-gun 58554, KoreaDepartment of Oriental Medicine Resources, Mokpo National University, Muan-gun 58554, KoreaDepartment of Oriental Medicine Resources, Mokpo National University, Muan-gun 58554, KoreaCollege of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-gun 58554, KoreaCollege of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-gun 58554, KoreaCardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes from <i>Boesenbergia rotunda</i> (L.) Mansf. [<i>Boesenbergia pandurata</i> (Roxb.) Schltr.] used as a spice and herbal medicine in Asian countries. The methanol extract of <i>B. rotunda</i> rhizomes (BRE) exhibited significant vasorelaxation effects ex vivo at EC<sub>50</sub> values of 13.4 ± 6.1 μg/mL and 40.9 ± 7.9 μg/mL, respectively, with and without endothelium in the porcine coronary artery ring. The intrinsic mechanism was evaluated by treating with specific inhibitors or activators that typically affect vascular reactivity. The results suggested that BRE induced relaxation in the coronary artery rings via an endothelium-dependent pathway involving NO-cGMP, and also via an endothelium-independent pathway involving the blockade of Ca<sup>2+</sup> channels. Vasorelaxant principles in BRE were identified by subsequent chromatographic methods, which revealed that flavonoids regulate vasorelaxant activity in BRE. One of the flavonoids was a Diels-Alder type adduct, 4-hydroxypanduratin A, which showed the most potent vasorelaxant effect on porcine coronary artery with an EC<sub>50</sub> of 17.8 ± 2.5 μM. Our results suggest that rhizomes of <i>B. rotunda</i> might be of interest as herbal medicine against cardiovascular diseases.https://www.mdpi.com/2223-7747/9/12/1688<i>Boesenbergia rotunda</i>Zingiberaceaeflavonoid4-hydroxypanduratinvasorelaxation |
| spellingShingle | Deepak Adhikari Dal-Seong Gong Se Hee Oh Eun Hee Sung Seung On Lee Dong-Wook Kim Min-Ho Oak Hyun Jung Kim Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary Artery Plants <i>Boesenbergia rotunda</i> Zingiberaceae flavonoid 4-hydroxypanduratin vasorelaxation |
| title | Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary Artery |
| title_full | Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary Artery |
| title_fullStr | Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary Artery |
| title_full_unstemmed | Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary Artery |
| title_short | Vasorelaxant Effect of <i>Boesenbergia rotunda</i> and Its Active Ingredients on an Isolated Coronary Artery |
| title_sort | vasorelaxant effect of i boesenbergia rotunda i and its active ingredients on an isolated coronary artery |
| topic | <i>Boesenbergia rotunda</i> Zingiberaceae flavonoid 4-hydroxypanduratin vasorelaxation |
| url | https://www.mdpi.com/2223-7747/9/12/1688 |
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