Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes
Clinical trials have demonstrated that heparan sulfate (HS) could be used as a therapeutic agent for the treatment of inflammatory diseases. Its anti-inflammatory effect makes it suitable for the development of biomimetic innovative strategies aiming at modulating stem cells behavior toward a pro-re...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fbioe.2017.00054/full |
| _version_ | 1811278599327580160 |
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| author | Bruna Corradetti Bruna Corradetti Francesca Taraballi Francesca Taraballi Ilaria Giretti Guillermo Bauza Guillermo Bauza Rossella S. Pistillo Federica Banche Niclot Federica Banche Niclot Laura Pandolfi Danilo Demarchi Ennio Tasciotti Ennio Tasciotti Ennio Tasciotti |
| author_facet | Bruna Corradetti Bruna Corradetti Francesca Taraballi Francesca Taraballi Ilaria Giretti Guillermo Bauza Guillermo Bauza Rossella S. Pistillo Federica Banche Niclot Federica Banche Niclot Laura Pandolfi Danilo Demarchi Ennio Tasciotti Ennio Tasciotti Ennio Tasciotti |
| author_sort | Bruna Corradetti |
| collection | DOAJ |
| description | Clinical trials have demonstrated that heparan sulfate (HS) could be used as a therapeutic agent for the treatment of inflammatory diseases. Its anti-inflammatory effect makes it suitable for the development of biomimetic innovative strategies aiming at modulating stem cells behavior toward a pro-regenerative phenotype in case of injury or inflammation. Here, we propose collagen type I meshes fabricated by solvent casting and further crosslinked with HS (HS-Col) to create a biomimetic environment resembling the extracellular matrix of soft tissue. HS-Col meshes were tested for their capability to provide physical support to stem cells’ growth, maintain their phenotypes and immunosuppressive potential following inflammation. HS-Col effect on stem cells was investigated in standard conditions as well as in an inflammatory environment recapitulated in vitro through a mix of pro-inflammatory cytokines (tumor necrosis factor-α and interferon-gamma; 20 ng/ml). A significant increase in the production of molecules associated with immunosuppression was demonstrated in response to the material and when cells were grown in presence of pro-inflammatory stimuli, compared to bare collagen membranes (Col), leading to a greater inhibitory potential when mesenchymal stem cells were exposed to stimulated peripheral blood mononuclear cells. Our data suggest that the presence of HS is able to activate the molecular machinery responsible for the release of anti-inflammatory cytokines, potentially leading to a faster resolution of inflammation. |
| first_indexed | 2024-04-13T00:38:49Z |
| format | Article |
| id | doaj.art-4210a8c430ab47af82efb9a6271fb27f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-13T00:38:49Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-4210a8c430ab47af82efb9a6271fb27f2022-12-22T03:10:16ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852017-09-01510.3389/fbioe.2017.00054262791Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory MembranesBruna Corradetti0Bruna Corradetti1Francesca Taraballi2Francesca Taraballi3Ilaria Giretti4Guillermo Bauza5Guillermo Bauza6Rossella S. Pistillo7Federica Banche Niclot8Federica Banche Niclot9Laura Pandolfi10Danilo Demarchi11Ennio Tasciotti12Ennio Tasciotti13Ennio Tasciotti14Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, United StatesDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, ItalyCenter for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, United StatesDepartment of Orthopaedic & Sports Medicine, The Houston Methodist Hospital, Houston, TX, United StatesDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, ItalyCenter for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, United StatesCenter for NanoHealth, Swansea University Medical School, Swansea University Bay, Wales, United KingdomDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, ItalyCenter for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, United StatesDepartment of Electronics and Telecommunications, Politecnico di Torino, Turin, ItalyCenter for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, United StatesDepartment of Electronics and Telecommunications, Politecnico di Torino, Turin, ItalyCenter for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, United StatesDepartment of Orthopaedic & Sports Medicine, The Houston Methodist Hospital, Houston, TX, United StatesCenter for NanoHealth, Swansea University Medical School, Swansea University Bay, Wales, United KingdomClinical trials have demonstrated that heparan sulfate (HS) could be used as a therapeutic agent for the treatment of inflammatory diseases. Its anti-inflammatory effect makes it suitable for the development of biomimetic innovative strategies aiming at modulating stem cells behavior toward a pro-regenerative phenotype in case of injury or inflammation. Here, we propose collagen type I meshes fabricated by solvent casting and further crosslinked with HS (HS-Col) to create a biomimetic environment resembling the extracellular matrix of soft tissue. HS-Col meshes were tested for their capability to provide physical support to stem cells’ growth, maintain their phenotypes and immunosuppressive potential following inflammation. HS-Col effect on stem cells was investigated in standard conditions as well as in an inflammatory environment recapitulated in vitro through a mix of pro-inflammatory cytokines (tumor necrosis factor-α and interferon-gamma; 20 ng/ml). A significant increase in the production of molecules associated with immunosuppression was demonstrated in response to the material and when cells were grown in presence of pro-inflammatory stimuli, compared to bare collagen membranes (Col), leading to a greater inhibitory potential when mesenchymal stem cells were exposed to stimulated peripheral blood mononuclear cells. Our data suggest that the presence of HS is able to activate the molecular machinery responsible for the release of anti-inflammatory cytokines, potentially leading to a faster resolution of inflammation.http://journal.frontiersin.org/article/10.3389/fbioe.2017.00054/fullmesenchymal stem cellsmicroenvironmentimmune responseregenerationtissue engineeringbiomolecules |
| spellingShingle | Bruna Corradetti Bruna Corradetti Francesca Taraballi Francesca Taraballi Ilaria Giretti Guillermo Bauza Guillermo Bauza Rossella S. Pistillo Federica Banche Niclot Federica Banche Niclot Laura Pandolfi Danilo Demarchi Ennio Tasciotti Ennio Tasciotti Ennio Tasciotti Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes Frontiers in Bioengineering and Biotechnology mesenchymal stem cells microenvironment immune response regeneration tissue engineering biomolecules |
| title | Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes |
| title_full | Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes |
| title_fullStr | Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes |
| title_full_unstemmed | Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes |
| title_short | Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes |
| title_sort | heparan sulfate a potential candidate for the development of biomimetic immunomodulatory membranes |
| topic | mesenchymal stem cells microenvironment immune response regeneration tissue engineering biomolecules |
| url | http://journal.frontiersin.org/article/10.3389/fbioe.2017.00054/full |
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