Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> Gene
The genomic analysis of all subspecies <i>F. tularensis</i>, as found in Gen Bank NCBI, reveals the presence of genes encoding proteins like to the multifunctional RecBCD enzyme complex in <i>E. coli</i> and other bacteria. To date, the role of the <i>recD</i> gen...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/1/108 |
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| author | Vitaly Pavlov Galina Vakhrameeva Alexander Mokrievich Mikhail E. Platonov Galina Titareva Raisa Mironova Tatiana Kombarova Tatiana Gapelchenkova Ivan Dyatlov |
| author_facet | Vitaly Pavlov Galina Vakhrameeva Alexander Mokrievich Mikhail E. Platonov Galina Titareva Raisa Mironova Tatiana Kombarova Tatiana Gapelchenkova Ivan Dyatlov |
| author_sort | Vitaly Pavlov |
| collection | DOAJ |
| description | The genomic analysis of all subspecies <i>F. tularensis</i>, as found in Gen Bank NCBI, reveals the presence of genes encoding proteins like to the multifunctional RecBCD enzyme complex in <i>E. coli</i> and other bacteria. To date, the role of the <i>recD</i> gene in <i>F. tularensis</i>, which encodes the alpha chain of exonuclease V, in DNA metabolism processes, has not been studied either in vitro or in vivo. <i>F. tularensis</i> subsp. <i>holarctica</i> 15 NIIEG, a vaccine strain, served as the basis to create the <i>F. tularensis</i> 15D strain with <i>recD</i> deletion. The lack of the <i>recD</i> gene suppresses the integration of suicide plasmids with <i>F. tularensis</i> genome fragments into the chromosome. The modified strain showed reduced growth in vitro and in vivo. This study shows that such deletion significantly reduces the virulence of the strain in BALB/c mice. |
| first_indexed | 2024-03-10T00:22:38Z |
| format | Article |
| id | doaj.art-4214f2c14c114cae8408ac3193b75452 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T00:22:38Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-4214f2c14c114cae8408ac3193b754522023-11-23T15:39:33ZengMDPI AGVaccines2076-393X2022-01-0110110810.3390/vaccines10010108Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> GeneVitaly Pavlov0Galina Vakhrameeva1Alexander Mokrievich2Mikhail E. Platonov3Galina Titareva4Raisa Mironova5Tatiana Kombarova6Tatiana Gapelchenkova7Ivan Dyatlov8State Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology (SRCAMB), 142279 Obolensk, RussiaThe genomic analysis of all subspecies <i>F. tularensis</i>, as found in Gen Bank NCBI, reveals the presence of genes encoding proteins like to the multifunctional RecBCD enzyme complex in <i>E. coli</i> and other bacteria. To date, the role of the <i>recD</i> gene in <i>F. tularensis</i>, which encodes the alpha chain of exonuclease V, in DNA metabolism processes, has not been studied either in vitro or in vivo. <i>F. tularensis</i> subsp. <i>holarctica</i> 15 NIIEG, a vaccine strain, served as the basis to create the <i>F. tularensis</i> 15D strain with <i>recD</i> deletion. The lack of the <i>recD</i> gene suppresses the integration of suicide plasmids with <i>F. tularensis</i> genome fragments into the chromosome. The modified strain showed reduced growth in vitro and in vivo. This study shows that such deletion significantly reduces the virulence of the strain in BALB/c mice.https://www.mdpi.com/2076-393X/10/1/108<i>Francisella tularensis</i> 15 NIIEG<i>recD</i> geneallelic exchangein vitro and in vivo reproductionBALB/c mice |
| spellingShingle | Vitaly Pavlov Galina Vakhrameeva Alexander Mokrievich Mikhail E. Platonov Galina Titareva Raisa Mironova Tatiana Kombarova Tatiana Gapelchenkova Ivan Dyatlov Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> Gene Vaccines <i>Francisella tularensis</i> 15 NIIEG <i>recD</i> gene allelic exchange in vitro and in vivo reproduction BALB/c mice |
| title | Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> Gene |
| title_full | Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> Gene |
| title_fullStr | Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> Gene |
| title_full_unstemmed | Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> Gene |
| title_short | Development and Properties of <i>Francisella tularensis</i> Subsp. <i>holarctica</i> 15 NIIEG Vaccine Strain without the <i>recD</i> Gene |
| title_sort | development and properties of i francisella tularensis i subsp i holarctica i 15 niieg vaccine strain without the i recd i gene |
| topic | <i>Francisella tularensis</i> 15 NIIEG <i>recD</i> gene allelic exchange in vitro and in vivo reproduction BALB/c mice |
| url | https://www.mdpi.com/2076-393X/10/1/108 |
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