Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4
Papillary thyroid carcinoma (PTC) is a differentiated type of thyroid malignancy with a high incidence. Long non-coding RNA (lncRNA) DUXAP8 has been reported to participate in the proliferation, migration, and invasion of several cancer types. However, its association with PTC has not yet been repor...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Bioengineered |
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| Online Access: | http://dx.doi.org/10.1080/21655979.2021.1882134 |
| _version_ | 1798034102933258240 |
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| author | Yan Liu Hejia Zhang Hui Wang Jiarui Du Peng Dong Meihan Liu Yuanqiang Lin |
| author_facet | Yan Liu Hejia Zhang Hui Wang Jiarui Du Peng Dong Meihan Liu Yuanqiang Lin |
| author_sort | Yan Liu |
| collection | DOAJ |
| description | Papillary thyroid carcinoma (PTC) is a differentiated type of thyroid malignancy with a high incidence. Long non-coding RNA (lncRNA) DUXAP8 has been reported to participate in the proliferation, migration, and invasion of several cancer types. However, its association with PTC has not yet been reported. The current study aimed to investigate the role of DUXAP8 in PTC and revealed the underlying mechanisms. The expression of DUXAP8 was knocked down in two PTC cell lines and the effects of DUXAP8 on the PTC biological behavior were examined by cell counting kit-8 (CCK-8), wound healing, and transwell invasion assays. Luciferase reporter assay was used to detect the binding activity between miR-223-3p and DUXAP8. We found that knockdown of DUXAP8 inhibited the proliferation, migration, and invasion of PTC cells. DUXAP8 could sponge miR-223-3p through the specific binding site. CXCR4 was a target of miR-223-3p. The malignant phenotypes of the PTC cells were suppressed by the over-expression of miR-223-3p. Moreover, miR-223-3p inhibition or CXCR4 over-expression partly restored the proliferation, migration, and invasion activities of DUXAP8-downregulated PTC cells. The results evidenced that DUXAP8 acted as an oncogene in PTC, these effects seemed to partly dependent on the miR-223-3p/CXCR4 axis. |
| first_indexed | 2024-04-11T20:39:44Z |
| format | Article |
| id | doaj.art-421a9f385ff24881967e5599b96ab7e9 |
| institution | Directory Open Access Journal |
| issn | 2165-5979 2165-5987 |
| language | English |
| last_indexed | 2024-04-11T20:39:44Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Bioengineered |
| spelling | doaj.art-421a9f385ff24881967e5599b96ab7e92022-12-22T04:04:14ZengTaylor & Francis GroupBioengineered2165-59792165-59872021-01-0112149650610.1080/21655979.2021.18821341882134Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4Yan Liu0Hejia Zhang1Hui Wang2Jiarui Du3Peng Dong4Meihan Liu5Yuanqiang Lin6China-Japan Union Hospital of Jilin UniversityChina-Japan Union Hospital of Jilin UniversityChina-Japan Union Hospital of Jilin UniversityChina-Japan Union Hospital of Jilin UniversityChina-Japan Union Hospital of Jilin UniversityChina-Japan Union Hospital of Jilin UniversityChina-Japan Union Hospital of Jilin UniversityPapillary thyroid carcinoma (PTC) is a differentiated type of thyroid malignancy with a high incidence. Long non-coding RNA (lncRNA) DUXAP8 has been reported to participate in the proliferation, migration, and invasion of several cancer types. However, its association with PTC has not yet been reported. The current study aimed to investigate the role of DUXAP8 in PTC and revealed the underlying mechanisms. The expression of DUXAP8 was knocked down in two PTC cell lines and the effects of DUXAP8 on the PTC biological behavior were examined by cell counting kit-8 (CCK-8), wound healing, and transwell invasion assays. Luciferase reporter assay was used to detect the binding activity between miR-223-3p and DUXAP8. We found that knockdown of DUXAP8 inhibited the proliferation, migration, and invasion of PTC cells. DUXAP8 could sponge miR-223-3p through the specific binding site. CXCR4 was a target of miR-223-3p. The malignant phenotypes of the PTC cells were suppressed by the over-expression of miR-223-3p. Moreover, miR-223-3p inhibition or CXCR4 over-expression partly restored the proliferation, migration, and invasion activities of DUXAP8-downregulated PTC cells. The results evidenced that DUXAP8 acted as an oncogene in PTC, these effects seemed to partly dependent on the miR-223-3p/CXCR4 axis.http://dx.doi.org/10.1080/21655979.2021.1882134papillary thyroid carcinomalong non-coding rna duxap8mir-223-3pcxcr4proliferationmigration and invasion |
| spellingShingle | Yan Liu Hejia Zhang Hui Wang Jiarui Du Peng Dong Meihan Liu Yuanqiang Lin Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4 Bioengineered papillary thyroid carcinoma long non-coding rna duxap8 mir-223-3p cxcr4 proliferation migration and invasion |
| title | Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4 |
| title_full | Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4 |
| title_fullStr | Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4 |
| title_full_unstemmed | Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4 |
| title_short | Long non-coding RNA DUXAP8 promotes the cell proliferation, migration, and invasion of papillary thyroid carcinoma via miR-223-3p mediated regulation of CXCR4 |
| title_sort | long non coding rna duxap8 promotes the cell proliferation migration and invasion of papillary thyroid carcinoma via mir 223 3p mediated regulation of cxcr4 |
| topic | papillary thyroid carcinoma long non-coding rna duxap8 mir-223-3p cxcr4 proliferation migration and invasion |
| url | http://dx.doi.org/10.1080/21655979.2021.1882134 |
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