Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis
Objective: To evaluate the sensitivity and specificity of three laboratory techniques for the diagnosis of Giardia intestinalis. Materials and Methods: 31 fecal samples from pre schoolers were submitted to microscopic examination with SSF-Lugol, the Ritchie concentration method and the “Giardia-Stri...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2014-08-01
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| Series: | Kasmera |
| Subjects: | |
| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/8653 |
| _version_ | 1827833920412975104 |
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| author | Marinella Calchi E. Acurero R. Villalobos M Colina L. Di Toro C. Villalobos |
| author_facet | Marinella Calchi E. Acurero R. Villalobos M Colina L. Di Toro C. Villalobos |
| author_sort | Marinella Calchi |
| collection | DOAJ |
| description | Objective: To evaluate the sensitivity and specificity of three laboratory techniques for the diagnosis of Giardia intestinalis. Materials and Methods: 31 fecal samples from pre schoolers were submitted to microscopic examination with SSF-Lugol, the Ritchie concentration method and the “Giardia-Strip” immunological method. The Ritchie technique was considered the “gold standard.“ Results: G. intestinalis cysts were identified in 6 samples (19.35%) using the Ritchie technique. The fresh test identified 5 cases of protozoan cysts (16.1%), showing a sensitivity of 83 % and specificity of 100 %. The Giardia-Strip identified cysts in 4 samples (12.9%) with 66.66 % sensitivity and 100% specificity. The positive predictive value for the “Giardia Strip” method was 14% and the negative predictive value was 93%. The fresh test showed a positive predic tive value of 100% and a negative predictive value of 96 %. Conclusions: Ritchie concentrate and the fresh test showed higher sensitivity and specificity in the diagnosis of Giardia intestinalis, when samples contain only cysts. However, the Giardia-Strip method proved faster in obtaining results. |
| first_indexed | 2024-03-12T05:41:22Z |
| format | Article |
| id | doaj.art-421acb53a57041cb9e366f0665195f98 |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T05:41:22Z |
| publishDate | 2014-08-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-421acb53a57041cb9e366f0665195f982023-09-03T06:00:33ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282014-08-01421324024376Comparison of Laboratory Techniques For the Diagnosis of Giardia IntestinalisMarinella Calchi0E. Acurero1R. Villalobos2M Colina3L. Di Toro4C. Villalobos5Cátedra de Parasitología. Escuela de Bioanálisis. Facultad de Medicina. Universidad del ZuliaCátedra de Parasitología. Escuela de Bioanálisis. Facultad de Medicina. Universidad del ZuliaCátedra de Medicina Tropical. Escuela de Bioanálisis. Facultad de Medicina. Universidad del ZuliaLicenciados en Bioanálisis. Escuela de Bioanálisis. Facultad de Medicina. Universidad del ZuliaLicenciados en Bioanálisis. Escuela de Bioanálisis. Facultad de Medicina. Universidad del ZuliaLicenciados en Bioanálisis. Escuela de Bioanálisis. Facultad de Medicina. Universidad del ZuliaObjective: To evaluate the sensitivity and specificity of three laboratory techniques for the diagnosis of Giardia intestinalis. Materials and Methods: 31 fecal samples from pre schoolers were submitted to microscopic examination with SSF-Lugol, the Ritchie concentration method and the “Giardia-Strip” immunological method. The Ritchie technique was considered the “gold standard.“ Results: G. intestinalis cysts were identified in 6 samples (19.35%) using the Ritchie technique. The fresh test identified 5 cases of protozoan cysts (16.1%), showing a sensitivity of 83 % and specificity of 100 %. The Giardia-Strip identified cysts in 4 samples (12.9%) with 66.66 % sensitivity and 100% specificity. The positive predictive value for the “Giardia Strip” method was 14% and the negative predictive value was 93%. The fresh test showed a positive predic tive value of 100% and a negative predictive value of 96 %. Conclusions: Ritchie concentrate and the fresh test showed higher sensitivity and specificity in the diagnosis of Giardia intestinalis, when samples contain only cysts. However, the Giardia-Strip method proved faster in obtaining results.https://produccioncientificaluz.org/index.php/kasmera/article/view/8653Giardiasis, Diagnóstico, Directo SSF-Lugol, Ritchie, Giardia-Strip |
| spellingShingle | Marinella Calchi E. Acurero R. Villalobos M Colina L. Di Toro C. Villalobos Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis Kasmera Giardiasis, Diagnóstico, Directo SSF-Lugol, Ritchie, Giardia-Strip |
| title | Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis |
| title_full | Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis |
| title_fullStr | Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis |
| title_full_unstemmed | Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis |
| title_short | Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis |
| title_sort | comparison of laboratory techniques for the diagnosis of giardia intestinalis |
| topic | Giardiasis, Diagnóstico, Directo SSF-Lugol, Ritchie, Giardia-Strip |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/8653 |
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