In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan Microparticles
This investigation provides experimental data related to the development of a useful in vitro model allowing for a deeper analysis of invasive (metastatic) cancer cells using the effect of forced acidosis on the behavior of an epithelial colon cancer cell line. The results demonstrate that incubatio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/23/12029 |
| _version_ | 1797463672325406720 |
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| author | Donika Ivanova Tanya Tacheva Severina Semkova Radmila Panovska Zvezdelina Yaneva |
| author_facet | Donika Ivanova Tanya Tacheva Severina Semkova Radmila Panovska Zvezdelina Yaneva |
| author_sort | Donika Ivanova |
| collection | DOAJ |
| description | This investigation provides experimental data related to the development of a useful in vitro model allowing for a deeper analysis of invasive (metastatic) cancer cells using the effect of forced acidosis on the behavior of an epithelial colon cancer cell line. The results demonstrate that incubation in the medium with different pH values, adjusted by hydrochloric or lactic acids, increased more than 20 fold the proliferative activity of cancer cells at 48 h of incubation without affecting their cell viability. The newly synthesized chitosan micro-formulations were tested as a potential system for the screening of cancer progression, based on the attachment of cancer cells to the chitosan surface, without radically disturbing their viability. The latter could allow us to develop a versatile and useful in vitro model for the detailed investigation of the biological and molecular biochemical processes of invasive (metastatic) cancer cells. |
| first_indexed | 2024-03-09T17:54:04Z |
| format | Article |
| id | doaj.art-421af07a4074494592ea072b416c2f35 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T17:54:04Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-421af07a4074494592ea072b416c2f352023-11-24T10:29:46ZengMDPI AGApplied Sciences2076-34172022-11-0112231202910.3390/app122312029In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan MicroparticlesDonika Ivanova0Tanya Tacheva1Severina Semkova2Radmila Panovska3Zvezdelina Yaneva4Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Chemistry and Biochemistry, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Electroinduced and Adhesive Properties, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, 1040 Sofia, BulgariaDepartment of Pediatrics, Medical Faculty, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaThis investigation provides experimental data related to the development of a useful in vitro model allowing for a deeper analysis of invasive (metastatic) cancer cells using the effect of forced acidosis on the behavior of an epithelial colon cancer cell line. The results demonstrate that incubation in the medium with different pH values, adjusted by hydrochloric or lactic acids, increased more than 20 fold the proliferative activity of cancer cells at 48 h of incubation without affecting their cell viability. The newly synthesized chitosan micro-formulations were tested as a potential system for the screening of cancer progression, based on the attachment of cancer cells to the chitosan surface, without radically disturbing their viability. The latter could allow us to develop a versatile and useful in vitro model for the detailed investigation of the biological and molecular biochemical processes of invasive (metastatic) cancer cells.https://www.mdpi.com/2076-3417/12/23/12029chitosan micro particlescancer cellsacidosiscancer progression |
| spellingShingle | Donika Ivanova Tanya Tacheva Severina Semkova Radmila Panovska Zvezdelina Yaneva In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan Microparticles Applied Sciences chitosan micro particles cancer cells acidosis cancer progression |
| title | In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan Microparticles |
| title_full | In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan Microparticles |
| title_fullStr | In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan Microparticles |
| title_full_unstemmed | In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan Microparticles |
| title_short | In Vitro Model for Evaluation of Cancer Cell Proliferative Activity under Simulated Acidosis and Using Chitosan Microparticles |
| title_sort | in vitro model for evaluation of cancer cell proliferative activity under simulated acidosis and using chitosan microparticles |
| topic | chitosan micro particles cancer cells acidosis cancer progression |
| url | https://www.mdpi.com/2076-3417/12/23/12029 |
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