Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells
Monitoring the thermal responses of individual cells to external stimuli is essential for studies of cell metabolism, organelle function, and drug screening. Fluorescent temperature probes are usually employed to measure the temperatures of individual cells; however, they have some unavoidable probl...
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MDPI AG
2021-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/11/454 |
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| author | Danhong Han Jingjing Xu Han Wang Zhenhai Wang Nana Yang Fan Yang Qundong Shen Shengyong Xu |
| author_facet | Danhong Han Jingjing Xu Han Wang Zhenhai Wang Nana Yang Fan Yang Qundong Shen Shengyong Xu |
| author_sort | Danhong Han |
| collection | DOAJ |
| description | Monitoring the thermal responses of individual cells to external stimuli is essential for studies of cell metabolism, organelle function, and drug screening. Fluorescent temperature probes are usually employed to measure the temperatures of individual cells; however, they have some unavoidable problems, such as, poor stability caused by their sensitivity to the chemical composition of the solution and the limitation in their measurement time due to the short fluorescence lifetime. Here, we demonstrate a stable, non-interventional, and high-precision temperature-measurement chip that can monitor the temperature fluctuations of individual cells subject to external stimuli and over a normal cell life cycle as long as several days. To improve the temperature resolution, we designed temperature sensors made of Pd–Cr thin-film thermocouples, a freestanding Si<sub>3</sub>N<sub>4</sub> platform, and a dual-temperature control system. Our experimental results confirm the feasibility of using this cellular temperature-measurement chip to detect local temperature fluctuations of individual cells that are 0.3–1.5 K higher than the ambient temperature for HeLa cells in different proliferation cycles. In the future, we plan to integrate this chip with other single-cell technologies and apply it to research related to cellular heat-stress response. |
| first_indexed | 2024-03-10T05:40:09Z |
| format | Article |
| id | doaj.art-421bd155de6e4e5083e6d1ccd5348d4a |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T05:40:09Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-421bd155de6e4e5083e6d1ccd5348d4a2023-11-22T22:36:24ZengMDPI AGBiosensors2079-63742021-11-01111145410.3390/bios11110454Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual CellsDanhong Han0Jingjing Xu1Han Wang2Zhenhai Wang3Nana Yang4Fan Yang5Qundong Shen6Shengyong Xu7Key Laboratory for the Physics & Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing 100871, ChinaKey Laboratory for the Physics & Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing 100871, ChinaDepartment of Orthopedics, Air Force Medical Center, Beijing 100142, ChinaKey Laboratory for the Physics & Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing 100871, ChinaKey Laboratory for the Physics & Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing 100871, ChinaKey Laboratory for the Physics & Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing 100871, ChinaDepartment of Chemistry, Nanjing University, Nanjing 210023, ChinaKey Laboratory for the Physics & Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing 100871, ChinaMonitoring the thermal responses of individual cells to external stimuli is essential for studies of cell metabolism, organelle function, and drug screening. Fluorescent temperature probes are usually employed to measure the temperatures of individual cells; however, they have some unavoidable problems, such as, poor stability caused by their sensitivity to the chemical composition of the solution and the limitation in their measurement time due to the short fluorescence lifetime. Here, we demonstrate a stable, non-interventional, and high-precision temperature-measurement chip that can monitor the temperature fluctuations of individual cells subject to external stimuli and over a normal cell life cycle as long as several days. To improve the temperature resolution, we designed temperature sensors made of Pd–Cr thin-film thermocouples, a freestanding Si<sub>3</sub>N<sub>4</sub> platform, and a dual-temperature control system. Our experimental results confirm the feasibility of using this cellular temperature-measurement chip to detect local temperature fluctuations of individual cells that are 0.3–1.5 K higher than the ambient temperature for HeLa cells in different proliferation cycles. In the future, we plan to integrate this chip with other single-cell technologies and apply it to research related to cellular heat-stress response.https://www.mdpi.com/2079-6374/11/11/454single-cell temperature measurementtemperature sensorsnon-interventional biosensorsbiochip fabricationcell temperaturecell metabolism |
| spellingShingle | Danhong Han Jingjing Xu Han Wang Zhenhai Wang Nana Yang Fan Yang Qundong Shen Shengyong Xu Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells Biosensors single-cell temperature measurement temperature sensors non-interventional biosensors biochip fabrication cell temperature cell metabolism |
| title | Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells |
| title_full | Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells |
| title_fullStr | Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells |
| title_full_unstemmed | Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells |
| title_short | Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells |
| title_sort | non interventional and high precision temperature measurement biochips for long term monitoring the temperature fluctuations of individual cells |
| topic | single-cell temperature measurement temperature sensors non-interventional biosensors biochip fabrication cell temperature cell metabolism |
| url | https://www.mdpi.com/2079-6374/11/11/454 |
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