Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide
Erwinia amylovora (E. amylovora) is the first bacterial plant pathogen described and demonstrated to cause fire blight, a devastating plant disease affecting a wide range of species including a wide variety of Rosaceae. In this study, we reported the lipopolysaccharide (LPS) core structure from E. a...
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MDPI AG
2017-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/18/3/559 |
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| author | Angela Casillo Marcello Ziaco Buko Lindner Susana Merino Elena Mendoza-Barberá Juan M. Tomás Maria Michela Corsaro |
| author_facet | Angela Casillo Marcello Ziaco Buko Lindner Susana Merino Elena Mendoza-Barberá Juan M. Tomás Maria Michela Corsaro |
| author_sort | Angela Casillo |
| collection | DOAJ |
| description | Erwinia amylovora (E. amylovora) is the first bacterial plant pathogen described and demonstrated to cause fire blight, a devastating plant disease affecting a wide range of species including a wide variety of Rosaceae. In this study, we reported the lipopolysaccharide (LPS) core structure from E. amylovora strain CFBP1430, the first one for an E. amylovora highly pathogenic strain. The chemical characterization was performed on the mutants waaL (lacking only the O-antigen LPS with a complete LPS-core), wabH and wabG (outer-LPS core mutants). The LPSs were isolated from dry cells and analyzed by means of chemical and spectroscopic methods. In particular, they were subjected to a mild acid hydrolysis and/or a hydrazinolysis and investigated in detail by one and two dimensional Nuclear Magnetic Resonance (NMR) spectroscopy and ElectroSpray Ionization Fourier Transform-Ion Cyclotron Resonance (ESI FT-ICR) mass spectrometry. |
| first_indexed | 2024-04-13T15:02:13Z |
| format | Article |
| id | doaj.art-421c75d950d147ce86d7e3b1f95f207c |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T15:02:13Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-421c75d950d147ce86d7e3b1f95f207c2022-12-22T02:42:15ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-03-0118355910.3390/ijms18030559ijms18030559Structural Characterization of Core Region in Erwinia amylovora LipopolysaccharideAngela Casillo0Marcello Ziaco1Buko Lindner2Susana Merino3Elena Mendoza-Barberá4Juan M. Tomás5Maria Michela Corsaro6Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, ItalyDivision of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, D-23845 Borstel, GermanyDepartment of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, SpainDepartment of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, SpainDepartment of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, SpainDepartment of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, ItalyErwinia amylovora (E. amylovora) is the first bacterial plant pathogen described and demonstrated to cause fire blight, a devastating plant disease affecting a wide range of species including a wide variety of Rosaceae. In this study, we reported the lipopolysaccharide (LPS) core structure from E. amylovora strain CFBP1430, the first one for an E. amylovora highly pathogenic strain. The chemical characterization was performed on the mutants waaL (lacking only the O-antigen LPS with a complete LPS-core), wabH and wabG (outer-LPS core mutants). The LPSs were isolated from dry cells and analyzed by means of chemical and spectroscopic methods. In particular, they were subjected to a mild acid hydrolysis and/or a hydrazinolysis and investigated in detail by one and two dimensional Nuclear Magnetic Resonance (NMR) spectroscopy and ElectroSpray Ionization Fourier Transform-Ion Cyclotron Resonance (ESI FT-ICR) mass spectrometry.http://www.mdpi.com/1422-0067/18/3/559lipopolysaccharideErwinia amylovoraNMRESI FT-ICRstructural determination |
| spellingShingle | Angela Casillo Marcello Ziaco Buko Lindner Susana Merino Elena Mendoza-Barberá Juan M. Tomás Maria Michela Corsaro Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide International Journal of Molecular Sciences lipopolysaccharide Erwinia amylovora NMR ESI FT-ICR structural determination |
| title | Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide |
| title_full | Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide |
| title_fullStr | Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide |
| title_full_unstemmed | Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide |
| title_short | Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide |
| title_sort | structural characterization of core region in erwinia amylovora lipopolysaccharide |
| topic | lipopolysaccharide Erwinia amylovora NMR ESI FT-ICR structural determination |
| url | http://www.mdpi.com/1422-0067/18/3/559 |
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