Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury
Objective(s): To evaluate the protective effect of erdosteine, an antiapoptotic and antioxidant agent, on torsion–detorsion evoked histopathological changes in experimental ovarian ischemia-reperfusion (IR) injury. Materials and Methods: Eighteen female Wistar albino rats were used in control, IR, a...
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| Format: | Article |
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Mashhad University of Medical Sciences
2017-01-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_8093_898d4ea5fa56354f2313dadba1e445e0.pdf |
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| author | Vedat Ugurel Ahmet Cagatay Cicek Mustafa Cemek Selim Demirtas A Tuba Kocaman Turan Karaca |
| author_facet | Vedat Ugurel Ahmet Cagatay Cicek Mustafa Cemek Selim Demirtas A Tuba Kocaman Turan Karaca |
| author_sort | Vedat Ugurel |
| collection | DOAJ |
| description | Objective(s): To evaluate the protective effect of erdosteine, an antiapoptotic and antioxidant agent, on torsion–detorsion evoked histopathological changes in experimental ovarian ischemia-reperfusion (IR) injury. Materials and Methods: Eighteen female Wistar albino rats were used in control, IR, and IR+Edosteine (IR-E) groups, (n=6 in each). The IR-E group received the erdosteine for seven days before the induction of torsion/retorsion, (10 mg/kg/days). The IR and IR-E groups were exposed to right unilateral adnexal torsion for 3 hr. Three hours later, re-laparotomy was performed, and the right ovaries were surgically excised. Oxidant and antioxidants levels were determined in serum. The ovarian tissue samples were received and fixed with 10% neutral buffered formalin. The sections were stained with H&E, anti-PCNA, and TUNEL. Results: The IR group were showed severe acute inflammation, polynuclear leukocytes and macrophages, stromal oedema and haemorrhage. Treatment with erdosteine in rats significantly retained degenerative changes in the ovaryPCNA (+) cell numbers were significantly decreased in the IR and IR-E groups unlike the control group. However, its numbers were significantly increased in the IR-E group unlike the IR group. TUNEL (+) cell numbers were significantly increased in the IR group unlike the control and the IR-E groups. In erdosteine treated group, TUNEL (+) cells were detected significantly less than the IR group (P |
| first_indexed | 2024-04-14T05:53:58Z |
| format | Article |
| id | doaj.art-421c92a7459e4c1c891fc23c8353a376 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-14T05:53:58Z |
| publishDate | 2017-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-421c92a7459e4c1c891fc23c8353a3762022-12-22T02:08:59ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742017-01-01201535810.22038/ijbms.2017.80938093Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injuryVedat Ugurel0Ahmet Cagatay Cicek1Mustafa Cemek2Selim Demirtas3A Tuba Kocaman4Turan Karaca5University of Trakya, Faculty of Medicine, Department of Obstetrics and Gynecology, 22030, Balkan Campus, Edirne, TurkeyUniversity of Trakya, Faculty of Medicine, Department of Histology and Embryology, 22030, Balkan Campus, Edirne, TurkeyYildiz Technical University, Biochemistry Division, Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Istanbul, TurkeyUniversity of Trakya, Faculty of Medicine, Department of Histology and Embryology, 22030, Balkan Campus, Edirne, TurkeyYildiz Technical University, Biochemistry Division, Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Istanbul, TurkeyUniversity of Trakya, Faculty of Medicine, Department of Histology and Embryology, 22030, Balkan Campus, Edirne, TurkeyObjective(s): To evaluate the protective effect of erdosteine, an antiapoptotic and antioxidant agent, on torsion–detorsion evoked histopathological changes in experimental ovarian ischemia-reperfusion (IR) injury. Materials and Methods: Eighteen female Wistar albino rats were used in control, IR, and IR+Edosteine (IR-E) groups, (n=6 in each). The IR-E group received the erdosteine for seven days before the induction of torsion/retorsion, (10 mg/kg/days). The IR and IR-E groups were exposed to right unilateral adnexal torsion for 3 hr. Three hours later, re-laparotomy was performed, and the right ovaries were surgically excised. Oxidant and antioxidants levels were determined in serum. The ovarian tissue samples were received and fixed with 10% neutral buffered formalin. The sections were stained with H&E, anti-PCNA, and TUNEL. Results: The IR group were showed severe acute inflammation, polynuclear leukocytes and macrophages, stromal oedema and haemorrhage. Treatment with erdosteine in rats significantly retained degenerative changes in the ovaryPCNA (+) cell numbers were significantly decreased in the IR and IR-E groups unlike the control group. However, its numbers were significantly increased in the IR-E group unlike the IR group. TUNEL (+) cell numbers were significantly increased in the IR group unlike the control and the IR-E groups. In erdosteine treated group, TUNEL (+) cells were detected significantly less than the IR group (Phttp://ijbms.mums.ac.ir/article_8093_898d4ea5fa56354f2313dadba1e445e0.pdfAntioxidantErdosteineIschemia-reperfusion OxidantOvaryRat |
| spellingShingle | Vedat Ugurel Ahmet Cagatay Cicek Mustafa Cemek Selim Demirtas A Tuba Kocaman Turan Karaca Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury Iranian Journal of Basic Medical Sciences Antioxidant Erdosteine Ischemia-reperfusion Oxidant Ovary Rat |
| title | Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury |
| title_full | Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury |
| title_fullStr | Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury |
| title_full_unstemmed | Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury |
| title_short | Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury |
| title_sort | antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia reperfusion injury |
| topic | Antioxidant Erdosteine Ischemia-reperfusion Oxidant Ovary Rat |
| url | http://ijbms.mums.ac.ir/article_8093_898d4ea5fa56354f2313dadba1e445e0.pdf |
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