Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows
Although hormonal induction of parturition in cattle results in the successful delivery of healthy calves, the risk of retained fetal membrane is significantly increased. In a previous study, a combination of the long-acting glucocorticoid, triamcinolone acetonide, with a high dose of betamethasone...
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2021-10-01
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| Series: | The Journal of Reproduction and Development |
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| Online Access: | https://www.jstage.jst.go.jp/article/jrd/68/1/68_2021-094/_pdf/-char/en |
| _version_ | 1797633928592359424 |
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| author | Ryo INABA Ryouka KAWAHARA-MIKI Akihisa SHINOZAWA Taichi YASUHARA Takashi FUJII Keisuke KOYAMA Michiko MURATA-OKUBO Kousaku SOUMA Hiroki HIRAYAMA |
| author_facet | Ryo INABA Ryouka KAWAHARA-MIKI Akihisa SHINOZAWA Taichi YASUHARA Takashi FUJII Keisuke KOYAMA Michiko MURATA-OKUBO Kousaku SOUMA Hiroki HIRAYAMA |
| author_sort | Ryo INABA |
| collection | DOAJ |
| description | Although hormonal induction of parturition in cattle results in the successful delivery of healthy calves, the risk of retained fetal membrane is significantly increased. In a previous study, a combination of the long-acting glucocorticoid, triamcinolone acetonide, with a high dose of betamethasone partially normalized the placentomal gene expression during parturition; however, the incidence of retained fetal membrane remained high. This study further explored placentomal dysfunction and aimed to elucidate the mechanism of retained fetal membrane in parturition-induced cows. In this study, transcriptome analysis revealed that enhanced glucocorticoid exposure normalized the expression of a substantial fraction of genes in the cotyledons. In contrast, a significant reduction in the multiple signaling pathway activities, including interferon signaling, was found in the caruncles during induced parturition. Real-time PCR showed that the expression of interferon-tau in the caruncles, but not interferon-alpha or interferon-gamma, was significantly lower in induced parturition than spontaneous parturition. Interferon-stimulated gene expression was also significantly decreased in the caruncles during induced parturition. These results indicate that interferon signaling could be important for immunological control in placentomes during parturition. Additionally, this suggests that interferon-tau might be a pivotal ligand for interferon receptors in the caruncles. This study revealed that peripheral blood leukocytes in prepartum cows transcribed interferon-tau. Macrophage infiltration in the placentome is known to participate in the detachment of the fetal membrane from the caruncle. Thus, this study raised the possibility that immune cells migrating into the caruncles at parturition may act as a source of ligands that activate interferon signaling. |
| first_indexed | 2024-03-11T12:01:33Z |
| format | Article |
| id | doaj.art-421d4856d6504ad8999032cc45579108 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T12:01:33Z |
| publishDate | 2021-10-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-421d4856d6504ad8999032cc455791082023-11-08T05:07:35ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002021-10-01681303710.1262/jrd.2021-094jrdImpaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cowsRyo INABA0Ryouka KAWAHARA-MIKI1Akihisa SHINOZAWA2Taichi YASUHARA3Takashi FUJII4Keisuke KOYAMA5Michiko MURATA-OKUBO6Kousaku SOUMA7Hiroki HIRAYAMA8Department of Bioproduction, Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanNODAI Genome Research Center, Tokyo University of Agriculture, Tokyo 156-8502, JapanNODAI Genome Research Center, Tokyo University of Agriculture, Tokyo 156-8502, JapanDepartment of Bioproduction, Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanAnimal Biotechnology Group, Animal Research Center, Hokkaido Research Organization, Shintoku, Hokkaido 081-0038, JapanDairy Cow Group, Daily Research Center, Hokkaido Research Organization, Nakashibetsu, Hokkaido 086-1135, JapanDepartment of Bioproduction, Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanDepartment of Bioproduction, Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanDepartment of Bioproduction, Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanAlthough hormonal induction of parturition in cattle results in the successful delivery of healthy calves, the risk of retained fetal membrane is significantly increased. In a previous study, a combination of the long-acting glucocorticoid, triamcinolone acetonide, with a high dose of betamethasone partially normalized the placentomal gene expression during parturition; however, the incidence of retained fetal membrane remained high. This study further explored placentomal dysfunction and aimed to elucidate the mechanism of retained fetal membrane in parturition-induced cows. In this study, transcriptome analysis revealed that enhanced glucocorticoid exposure normalized the expression of a substantial fraction of genes in the cotyledons. In contrast, a significant reduction in the multiple signaling pathway activities, including interferon signaling, was found in the caruncles during induced parturition. Real-time PCR showed that the expression of interferon-tau in the caruncles, but not interferon-alpha or interferon-gamma, was significantly lower in induced parturition than spontaneous parturition. Interferon-stimulated gene expression was also significantly decreased in the caruncles during induced parturition. These results indicate that interferon signaling could be important for immunological control in placentomes during parturition. Additionally, this suggests that interferon-tau might be a pivotal ligand for interferon receptors in the caruncles. This study revealed that peripheral blood leukocytes in prepartum cows transcribed interferon-tau. Macrophage infiltration in the placentome is known to participate in the detachment of the fetal membrane from the caruncle. Thus, this study raised the possibility that immune cells migrating into the caruncles at parturition may act as a source of ligands that activate interferon signaling.https://www.jstage.jst.go.jp/article/jrd/68/1/68_2021-094/_pdf/-char/encattleinterferonparturitionplacentaretained fetal membrane |
| spellingShingle | Ryo INABA Ryouka KAWAHARA-MIKI Akihisa SHINOZAWA Taichi YASUHARA Takashi FUJII Keisuke KOYAMA Michiko MURATA-OKUBO Kousaku SOUMA Hiroki HIRAYAMA Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows The Journal of Reproduction and Development cattle interferon parturition placenta retained fetal membrane |
| title | Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows |
| title_full | Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows |
| title_fullStr | Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows |
| title_full_unstemmed | Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows |
| title_short | Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows |
| title_sort | impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition induced cows |
| topic | cattle interferon parturition placenta retained fetal membrane |
| url | https://www.jstage.jst.go.jp/article/jrd/68/1/68_2021-094/_pdf/-char/en |
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