Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause
Background. Melatonin, N-acetyl-5-methoxy-tryptamine is the major secretion product of the pineal gland with important anti-inflammatory and antioxidant properties, also being an im-portant marker of bone remodelling associated with menopause. Objectives. The aim of our study was to evaluate the eff...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Romanian Association of Balneology, Editura Balneara
2022-03-01
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| Series: | Balneo and PRM Research Journal |
| Subjects: | |
| Online Access: | http://bioclima.ro/Balneo493.pdf |
| _version_ | 1818445754661863424 |
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| author | Viorela Mihaela Ciortea Monica Ileana Borda Irina Motoașcă Sergiu Șușman Alina Deniza Ciubean Alina Liliana Pintea Rodica Ana Ungur Mădălina Gabriela Iliescu Laszlo Irsay |
| author_facet | Viorela Mihaela Ciortea Monica Ileana Borda Irina Motoașcă Sergiu Șușman Alina Deniza Ciubean Alina Liliana Pintea Rodica Ana Ungur Mădălina Gabriela Iliescu Laszlo Irsay |
| author_sort | Viorela Mihaela Ciortea |
| collection | DOAJ |
| description | Background. Melatonin, N-acetyl-5-methoxy-tryptamine is the major secretion product of the pineal gland with important anti-inflammatory and antioxidant properties, also being an im-portant marker of bone remodelling associated with menopause. Objectives. The aim of our study was to evaluate the effect of the co-administration of melatonin and estrogen on systemic inflammatory status and bone histopathological modifications in surgically induced menopau-sal female rats. Materials and methods. The study was performed on a number of 40 female rats, Wistar breed, which underwent bilateral surgical ovariectomy. Within 14 days postoperative, hormone replacement therapy with estrogen or estrogen with melatonin was initiated, in differ-ent doses. The treatment was administered for 12 consecutive weeks. At the end of the treatment we measured the serum levels of IL-6 and TNF-α. The femoral bones were harvested after sacri-ficing the animals and the thickness of the cortical bones was measured and histologically ana-lysed.
Results. Serum values of inflammatory markers were negatively correlated with melatonin ad-ministration, the differences being more important at higher doses of melatonin (for both IL-6 and TNF-α the difference between group E_2M with estrogen substitution and melatonin in double dose and control group W, without hormone replacement, was highly statistically signif-icant with p <0.0001). Bone diameters improved in the case of female rats that received hormone replacement with estrogen and higher dose of melatonin (p = 0.0004 between group E_2M, with hormone replacement and group W, control group). Conclusions. Melatonin improved inflam-matory status and bone histopathological changes in ovariectomized female rats.
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| first_indexed | 2024-12-14T19:36:51Z |
| format | Article |
| id | doaj.art-422208e035ab4654ad7ab3134f1e95e9 |
| institution | Directory Open Access Journal |
| issn | 2734-8458 |
| language | English |
| last_indexed | 2024-12-14T19:36:51Z |
| publishDate | 2022-03-01 |
| publisher | Romanian Association of Balneology, Editura Balneara |
| record_format | Article |
| series | Balneo and PRM Research Journal |
| spelling | doaj.art-422208e035ab4654ad7ab3134f1e95e92022-12-21T22:49:51ZengRomanian Association of Balneology, Editura BalnearaBalneo and PRM Research Journal2734-84582022-03-0113149310.12680/balneo.2022.493Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopauseViorela Mihaela Ciortea0Monica Ileana Borda1Irina Motoașcă2Sergiu Șușman3Alina Deniza Ciubean 4Alina Liliana Pintea5Rodica Ana Ungur6Mădălina Gabriela Iliescu7Laszlo Irsay81 Department of Rehabilitation, ”Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, Romania 2 Clinical Rehabilitation Hospital Cluj-Napoca, Romania1 Department of Rehabilitation, ”Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, Romania 2 Clinical Rehabilitation Hospital Cluj-Napoca, RomaniaClinical Rehabilitation Hospital Cluj-Napoca, RomaniaDepartment of Histology, ”Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, RomaniaDepartment of Rehabilitation, ”Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, RomaniaDental Medicine and Nursing Department, ”Lucian Blaga” University of Sibiu, Faculty of Medi-cine, Academic Emergency Hospital of Sibiu, Romania1 Department of Rehabilitation, ”Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, Romania 2 Clinical Rehabilitation Hospital Cluj-Napoca, RomaniaDepartment of rehabilitation, Faculty of Medicine Ovidius University of Constanta, Romania1 Department of Rehabilitation, ”Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, Romania 2 Clinical Rehabilitation Hospital Cluj-Napoca, RomaniaBackground. Melatonin, N-acetyl-5-methoxy-tryptamine is the major secretion product of the pineal gland with important anti-inflammatory and antioxidant properties, also being an im-portant marker of bone remodelling associated with menopause. Objectives. The aim of our study was to evaluate the effect of the co-administration of melatonin and estrogen on systemic inflammatory status and bone histopathological modifications in surgically induced menopau-sal female rats. Materials and methods. The study was performed on a number of 40 female rats, Wistar breed, which underwent bilateral surgical ovariectomy. Within 14 days postoperative, hormone replacement therapy with estrogen or estrogen with melatonin was initiated, in differ-ent doses. The treatment was administered for 12 consecutive weeks. At the end of the treatment we measured the serum levels of IL-6 and TNF-α. The femoral bones were harvested after sacri-ficing the animals and the thickness of the cortical bones was measured and histologically ana-lysed. Results. Serum values of inflammatory markers were negatively correlated with melatonin ad-ministration, the differences being more important at higher doses of melatonin (for both IL-6 and TNF-α the difference between group E_2M with estrogen substitution and melatonin in double dose and control group W, without hormone replacement, was highly statistically signif-icant with p <0.0001). Bone diameters improved in the case of female rats that received hormone replacement with estrogen and higher dose of melatonin (p = 0.0004 between group E_2M, with hormone replacement and group W, control group). Conclusions. Melatonin improved inflam-matory status and bone histopathological changes in ovariectomized female rats. http://bioclima.ro/Balneo493.pdfmelatoninestrogen replacement therapyinflammationlow bone density |
| spellingShingle | Viorela Mihaela Ciortea Monica Ileana Borda Irina Motoașcă Sergiu Șușman Alina Deniza Ciubean Alina Liliana Pintea Rodica Ana Ungur Mădălina Gabriela Iliescu Laszlo Irsay Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause Balneo and PRM Research Journal melatonin estrogen replacement therapy inflammation low bone density |
| title | Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause |
| title_full | Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause |
| title_fullStr | Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause |
| title_full_unstemmed | Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause |
| title_short | Influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause |
| title_sort | influence of melatonin on systemic inflammatory status and bone histopathological modifications in female rats with surgically induced menopause |
| topic | melatonin estrogen replacement therapy inflammation low bone density |
| url | http://bioclima.ro/Balneo493.pdf |
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