A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it>
<p>Abstract</p> <p>Background</p> <p>Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium <it>Corynebacterium ps...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-01-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/11/12 |
| _version_ | 1818919352784650240 |
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| author | Scrivens James H Silva Artur Meyer Roberto Pimenta Adriano MC Carvalho Maria AR Farias Luiz M Santos Simone G Santos Agenor V Silva Wanderson M Castro Thiago LP Santos Anderson R Seyffert Núbia Slade Susan E Pacheco Luis GC Oliveira Sérgio C Miyoshi Anderson Dowson Christopher G Azevedo Vasco |
| author_facet | Scrivens James H Silva Artur Meyer Roberto Pimenta Adriano MC Carvalho Maria AR Farias Luiz M Santos Simone G Santos Agenor V Silva Wanderson M Castro Thiago LP Santos Anderson R Seyffert Núbia Slade Susan E Pacheco Luis GC Oliveira Sérgio C Miyoshi Anderson Dowson Christopher G Azevedo Vasco |
| author_sort | Scrivens James H |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium <it>Corynebacterium pseudotuberculosis</it>, the etiological agent of caseous lymphadenitis (CLA) in sheep and goats.</p> <p>Results</p> <p>An optimized protocol of three-phase partitioning (TPP) was used to obtain the <it>C. pseudotuberculosis </it>exoproteins, and a newly introduced method of data-independent MS acquisition (LC-MS<sup>E</sup>) was employed for protein identification and label-free quantification. Additionally, the recently developed tool SurfG+ was used for <it>in silico </it>prediction of sub-cellular localization of the identified proteins. In total, 93 different extracellular proteins of <it>C. pseudotuberculosis </it>were identified with high confidence by this strategy; 44 proteins were commonly identified in two different strains, isolated from distinct hosts, then composing a core <it>C. pseudotuberculosis </it>exoproteome. Analysis with the SurfG+ tool showed that more than 75% (70/93) of the identified proteins could be predicted as containing signals for active exportation. Moreover, evidence could be found for probable non-classical export of most of the remaining proteins.</p> <p>Conclusions</p> <p>Comparative analyses of the exoproteomes of two <it>C. pseudotuberculosis </it>strains, in addition to comparison with other experimentally determined corynebacterial exoproteomes, were helpful to gain novel insights into the contribution of the exported proteins in the virulence of this bacterium. The results presented here compose the most comprehensive coverage of the exoproteome of a corynebacterial species so far.</p> |
| first_indexed | 2024-12-20T01:04:30Z |
| format | Article |
| id | doaj.art-422227016c004dbfb0bc193c822f52d8 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-20T01:04:30Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-422227016c004dbfb0bc193c822f52d82022-12-21T19:58:53ZengBMCBMC Microbiology1471-21802011-01-011111210.1186/1471-2180-11-12A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it>Scrivens James HSilva ArturMeyer RobertoPimenta Adriano MCCarvalho Maria ARFarias Luiz MSantos Simone GSantos Agenor VSilva Wanderson MCastro Thiago LPSantos Anderson RSeyffert NúbiaSlade Susan EPacheco Luis GCOliveira Sérgio CMiyoshi AndersonDowson Christopher GAzevedo Vasco<p>Abstract</p> <p>Background</p> <p>Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium <it>Corynebacterium pseudotuberculosis</it>, the etiological agent of caseous lymphadenitis (CLA) in sheep and goats.</p> <p>Results</p> <p>An optimized protocol of three-phase partitioning (TPP) was used to obtain the <it>C. pseudotuberculosis </it>exoproteins, and a newly introduced method of data-independent MS acquisition (LC-MS<sup>E</sup>) was employed for protein identification and label-free quantification. Additionally, the recently developed tool SurfG+ was used for <it>in silico </it>prediction of sub-cellular localization of the identified proteins. In total, 93 different extracellular proteins of <it>C. pseudotuberculosis </it>were identified with high confidence by this strategy; 44 proteins were commonly identified in two different strains, isolated from distinct hosts, then composing a core <it>C. pseudotuberculosis </it>exoproteome. Analysis with the SurfG+ tool showed that more than 75% (70/93) of the identified proteins could be predicted as containing signals for active exportation. Moreover, evidence could be found for probable non-classical export of most of the remaining proteins.</p> <p>Conclusions</p> <p>Comparative analyses of the exoproteomes of two <it>C. pseudotuberculosis </it>strains, in addition to comparison with other experimentally determined corynebacterial exoproteomes, were helpful to gain novel insights into the contribution of the exported proteins in the virulence of this bacterium. The results presented here compose the most comprehensive coverage of the exoproteome of a corynebacterial species so far.</p>http://www.biomedcentral.com/1471-2180/11/12 |
| spellingShingle | Scrivens James H Silva Artur Meyer Roberto Pimenta Adriano MC Carvalho Maria AR Farias Luiz M Santos Simone G Santos Agenor V Silva Wanderson M Castro Thiago LP Santos Anderson R Seyffert Núbia Slade Susan E Pacheco Luis GC Oliveira Sérgio C Miyoshi Anderson Dowson Christopher G Azevedo Vasco A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it> BMC Microbiology |
| title | A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it> |
| title_full | A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it> |
| title_fullStr | A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it> |
| title_full_unstemmed | A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it> |
| title_short | A combined approach for comparative exoproteome analysis of <it>Corynebacterium pseudotuberculosis</it> |
| title_sort | combined approach for comparative exoproteome analysis of it corynebacterium pseudotuberculosis it |
| url | http://www.biomedcentral.com/1471-2180/11/12 |
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