Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains
Characterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild type and mutant Weissella confusa was investigated. The EPS production ranged from 5490.2 to 5580.7 mg/L. Wild type Weissella confusa (WWCEPS) had the highest EPS production. Eight (8) sugar moie...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-09-01
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| Series: | Biotechnology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X18301292 |
| _version_ | 1819063329360969728 |
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| author | Bukola Adebayo-Tayo Racheal Ishola Titiloye Oyewunmi |
| author_facet | Bukola Adebayo-Tayo Racheal Ishola Titiloye Oyewunmi |
| author_sort | Bukola Adebayo-Tayo |
| collection | DOAJ |
| description | Characterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild type and mutant Weissella confusa was investigated. The EPS production ranged from 5490.2 to 5580.7 mg/L. Wild type Weissella confusa (WWCEPS) had the highest EPS production. Eight (8) sugar moieties were present in the EPS. Galactose had the highest EPS composition (34.6 mg/100 g and 33.5 mg/100 g EPS) in Wild type Weissella confusa EPS (WWCEPS) and mutant Weissella confusa EPS (MWCEPS). Wild type Weissella confusa and mutant Weissella confusa EPS had antioxidant capacity. The scavenging assay for the antioxidant increased in a dose dependent (0.5–10 mg/mL) manner. Wild type Weissella confusa EPS had the highest 1,1-Diphemy 1-2-picryl-hydrazyl (DPPH) capacity, total antioxidant activity, hydrogen peroxide and reducing power activity (71%, 1.9%, 86.7% and 1.9%). The mice injected peritorially with mutant Weissella confusa EPS had the highest IgG and IgM (68–87 mg/dL and 64–70 mg/dL). IgA of the mice treated with Wild type Weissella confusa EPS increased from 67 to 73 mg/dL. Wild type and mutant Weissella confusa EPS had immunomodulatory activity on the treated mice. Keywords: Exopolysaccharide, Immunomodulatory, Antioxidant, Immunoglobulin, Lactic acid bacteria |
| first_indexed | 2024-12-21T15:12:57Z |
| format | Article |
| id | doaj.art-4222bcb9841f493ba1de5838d2c41fc3 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-21T15:12:57Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-4222bcb9841f493ba1de5838d2c41fc32022-12-21T18:59:14ZengElsevierBiotechnology Reports2215-017X2018-09-0119Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strainsBukola Adebayo-Tayo0Racheal Ishola1Titiloye Oyewunmi2Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria; Corresponding author.Department of Microbiology, University of Ibadan, Ibadan, Oyo State, NigeriaImmunology Unit, Department of Chemical Pathology, College of Medicine, University of Ibadan, NigeriaCharacterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild type and mutant Weissella confusa was investigated. The EPS production ranged from 5490.2 to 5580.7 mg/L. Wild type Weissella confusa (WWCEPS) had the highest EPS production. Eight (8) sugar moieties were present in the EPS. Galactose had the highest EPS composition (34.6 mg/100 g and 33.5 mg/100 g EPS) in Wild type Weissella confusa EPS (WWCEPS) and mutant Weissella confusa EPS (MWCEPS). Wild type Weissella confusa and mutant Weissella confusa EPS had antioxidant capacity. The scavenging assay for the antioxidant increased in a dose dependent (0.5–10 mg/mL) manner. Wild type Weissella confusa EPS had the highest 1,1-Diphemy 1-2-picryl-hydrazyl (DPPH) capacity, total antioxidant activity, hydrogen peroxide and reducing power activity (71%, 1.9%, 86.7% and 1.9%). The mice injected peritorially with mutant Weissella confusa EPS had the highest IgG and IgM (68–87 mg/dL and 64–70 mg/dL). IgA of the mice treated with Wild type Weissella confusa EPS increased from 67 to 73 mg/dL. Wild type and mutant Weissella confusa EPS had immunomodulatory activity on the treated mice. Keywords: Exopolysaccharide, Immunomodulatory, Antioxidant, Immunoglobulin, Lactic acid bacteriahttp://www.sciencedirect.com/science/article/pii/S2215017X18301292 |
| spellingShingle | Bukola Adebayo-Tayo Racheal Ishola Titiloye Oyewunmi Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains Biotechnology Reports |
| title | Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains |
| title_full | Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains |
| title_fullStr | Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains |
| title_full_unstemmed | Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains |
| title_short | Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains |
| title_sort | characterization antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant weissella confusa strains |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X18301292 |
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