Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination
Dengue virus (DENV) antibody assays frequently cross-react with sera from individuals who have been infected with or vaccinated against related flaviviruses. The goal of this study was to determine the specificity of two DENV ELISAs with sera from individuals vaccinated against yellow fever virus (Y...
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MDPI AG
2022-12-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/8/1/7 |
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| author | Isabelle Schnabel Sophie Schneitler Tom Schüttoff Henning Trawinski Christoph Lübbert Christian Jassoy |
| author_facet | Isabelle Schnabel Sophie Schneitler Tom Schüttoff Henning Trawinski Christoph Lübbert Christian Jassoy |
| author_sort | Isabelle Schnabel |
| collection | DOAJ |
| description | Dengue virus (DENV) antibody assays frequently cross-react with sera from individuals who have been infected with or vaccinated against related flaviviruses. The goal of this study was to determine the specificity of two DENV ELISAs with sera from individuals vaccinated against yellow fever virus (YFV) and Japanese encephalitis virus (JEV). The Panbio and the Novatec Dengue IgG ELISAs were tested with sera obtained 3–4 weeks or 0.5–6 years after YFV or JEV vaccination and the diagnostic specificity of the assays was determined. As controls, the sera were tested using DENV, YFV, JEV, Zika and West Nile virus neutralization assays. The diagnostic specificity of the Panbio and the Novatec ELISA with sera from YFV-vaccinated subjects was 98.2% and 88.2%, respectively. Cross-reactions were rare in the first 4 weeks despite high YFV-neutralizing antibody titers and were mostly found later. The specificity of the Panbio and Novatec assays with sera from JEV-vaccinated individuals was 100% and 92.9%. Cross-reactions occurred in the early time period after vaccination. The measurement values of the two ELISAs correlated strongly. Thus, the Panbio ELISA showed higher diagnostic specificity and may be suitable for seroprevalence studies in areas with high disease prevalence. |
| first_indexed | 2024-03-09T11:05:59Z |
| format | Article |
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| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-09T11:05:59Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-422338633c3a4edf820fca4ded43967b2023-12-01T00:57:43ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-12-0181710.3390/tropicalmed8010007Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus VaccinationIsabelle Schnabel0Sophie Schneitler1Tom Schüttoff2Henning Trawinski3Christoph Lübbert4Christian Jassoy5Institute for Medical Microbiology and Virology, University Hospital and Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyDivision of Infectious Diseases and Tropical Medicine, Department of Medicine, University Hospital and Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyInstitute for Medical Microbiology and Virology, University Hospital and Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyDivision of Infectious Diseases and Tropical Medicine, Department of Medicine, University Hospital and Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyDivision of Infectious Diseases and Tropical Medicine, Department of Medicine, University Hospital and Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyInstitute for Medical Microbiology and Virology, University Hospital and Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyDengue virus (DENV) antibody assays frequently cross-react with sera from individuals who have been infected with or vaccinated against related flaviviruses. The goal of this study was to determine the specificity of two DENV ELISAs with sera from individuals vaccinated against yellow fever virus (YFV) and Japanese encephalitis virus (JEV). The Panbio and the Novatec Dengue IgG ELISAs were tested with sera obtained 3–4 weeks or 0.5–6 years after YFV or JEV vaccination and the diagnostic specificity of the assays was determined. As controls, the sera were tested using DENV, YFV, JEV, Zika and West Nile virus neutralization assays. The diagnostic specificity of the Panbio and the Novatec ELISA with sera from YFV-vaccinated subjects was 98.2% and 88.2%, respectively. Cross-reactions were rare in the first 4 weeks despite high YFV-neutralizing antibody titers and were mostly found later. The specificity of the Panbio and Novatec assays with sera from JEV-vaccinated individuals was 100% and 92.9%. Cross-reactions occurred in the early time period after vaccination. The measurement values of the two ELISAs correlated strongly. Thus, the Panbio ELISA showed higher diagnostic specificity and may be suitable for seroprevalence studies in areas with high disease prevalence.https://www.mdpi.com/2414-6366/8/1/7dengue virusflavivirusELISAantibody cross-reactiondiagnostic specificity |
| spellingShingle | Isabelle Schnabel Sophie Schneitler Tom Schüttoff Henning Trawinski Christoph Lübbert Christian Jassoy Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination Tropical Medicine and Infectious Disease dengue virus flavivirus ELISA antibody cross-reaction diagnostic specificity |
| title | Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination |
| title_full | Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination |
| title_fullStr | Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination |
| title_full_unstemmed | Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination |
| title_short | Diagnostic Specificity of Two Dengue Virus IgG ELISAs after Yellow Fever and Japanese Encephalitis Virus Vaccination |
| title_sort | diagnostic specificity of two dengue virus igg elisas after yellow fever and japanese encephalitis virus vaccination |
| topic | dengue virus flavivirus ELISA antibody cross-reaction diagnostic specificity |
| url | https://www.mdpi.com/2414-6366/8/1/7 |
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