A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes
Bacteria are similar to social organisms that engage in critical interactions with one another, forming spatially structured communities. Despite extensive research on the composition, structure, and communication of bacteria, the mechanisms behind their interactions and biofilm formation are not ye...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/7/695 |
| _version_ | 1797590021785518080 |
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| author | Tzu-En Lin Sorour Darvishi |
| author_facet | Tzu-En Lin Sorour Darvishi |
| author_sort | Tzu-En Lin |
| collection | DOAJ |
| description | Bacteria are similar to social organisms that engage in critical interactions with one another, forming spatially structured communities. Despite extensive research on the composition, structure, and communication of bacteria, the mechanisms behind their interactions and biofilm formation are not yet fully understood. To address this issue, scanning probe techniques such as atomic force microscopy (AFM), scanning electrochemical microscopy (SECM), scanning electrochemical cell microscopy (SECCM), and scanning ion-conductance microscopy (SICM) have been utilized to analyze bacteria. This review article focuses on summarizing the use of electrochemical scanning probes for investigating bacteria, including analysis of electroactive metabolites, enzymes, oxygen consumption, ion concentrations, pH values, biofilms, and quorum sensing molecules to provide a better understanding of bacterial interactions and communication. SECM has been combined with other techniques, such as AFM, inverted optical microscopy, SICM, and fluorescence microscopy. This allows a comprehensive study of the surfaces of bacteria while also providing more information on their metabolic activity. In general, the use of scanning probes for the detection of bacteria has shown great promise and has the potential to provide a powerful tool for the study of bacterial physiology and the detection of bacterial infections. |
| first_indexed | 2024-03-11T01:15:25Z |
| format | Article |
| id | doaj.art-4227f5c3eff7452ea113a13606e53447 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T01:15:25Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-4227f5c3eff7452ea113a13606e534472023-11-18T18:32:41ZengMDPI AGBiosensors2079-63742023-06-0113769510.3390/bios13070695A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning ProbesTzu-En Lin0Sorour Darvishi1Institute of Biomedical Engineering, Department of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, TaiwanDepartment of Electrical Engineering and Computer Sciences, University of California, Berkeley, CA 94720, USABacteria are similar to social organisms that engage in critical interactions with one another, forming spatially structured communities. Despite extensive research on the composition, structure, and communication of bacteria, the mechanisms behind their interactions and biofilm formation are not yet fully understood. To address this issue, scanning probe techniques such as atomic force microscopy (AFM), scanning electrochemical microscopy (SECM), scanning electrochemical cell microscopy (SECCM), and scanning ion-conductance microscopy (SICM) have been utilized to analyze bacteria. This review article focuses on summarizing the use of electrochemical scanning probes for investigating bacteria, including analysis of electroactive metabolites, enzymes, oxygen consumption, ion concentrations, pH values, biofilms, and quorum sensing molecules to provide a better understanding of bacterial interactions and communication. SECM has been combined with other techniques, such as AFM, inverted optical microscopy, SICM, and fluorescence microscopy. This allows a comprehensive study of the surfaces of bacteria while also providing more information on their metabolic activity. In general, the use of scanning probes for the detection of bacteria has shown great promise and has the potential to provide a powerful tool for the study of bacterial physiology and the detection of bacterial infections.https://www.mdpi.com/2079-6374/13/7/695scanning electrochemical microscopyquorum sensingmetabolic activityoxygen respirationelectrochemical active metabolitesoxygen consumption |
| spellingShingle | Tzu-En Lin Sorour Darvishi A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes Biosensors scanning electrochemical microscopy quorum sensing metabolic activity oxygen respiration electrochemical active metabolites oxygen consumption |
| title | A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes |
| title_full | A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes |
| title_fullStr | A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes |
| title_full_unstemmed | A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes |
| title_short | A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes |
| title_sort | brief review of in situ and operando electrochemical analysis of bacteria by scanning probes |
| topic | scanning electrochemical microscopy quorum sensing metabolic activity oxygen respiration electrochemical active metabolites oxygen consumption |
| url | https://www.mdpi.com/2079-6374/13/7/695 |
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