CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS Patients
Feng Qian,1– 3,* Song Hu,4,* Yueping Zhu,2,3 Yinling Wang,2,3 Jin Liu,2,3 Jialu Qiao,4 Xiji Shu,4 Yong Gao,5 Binlian Sun,4 Chuanwu Zhu1– 3 1Department of Infectious Diseases, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People’s Republic of China; 2Department...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-05-01
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| Series: | International Journal of General Medicine |
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| Online Access: | https://www.dovepress.com/cd56dim-nk-cell-is-an-important-factor-in-t-cell-depletion-of-cart-tre-peer-reviewed-fulltext-article-IJGM |
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| author | Qian F Hu S Zhu Y Wang Y Liu J Qiao J Shu X Gao Y Sun B Zhu C |
| author_facet | Qian F Hu S Zhu Y Wang Y Liu J Qiao J Shu X Gao Y Sun B Zhu C |
| author_sort | Qian F |
| collection | DOAJ |
| description | Feng Qian,1– 3,* Song Hu,4,* Yueping Zhu,2,3 Yinling Wang,2,3 Jin Liu,2,3 Jialu Qiao,4 Xiji Shu,4 Yong Gao,5 Binlian Sun,4 Chuanwu Zhu1– 3 1Department of Infectious Diseases, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People’s Republic of China; 2Department of Infectious Diseases, The Affiliated Infectious Disease Hospital of Soochow University, Suzhou, 215131, People’s Republic of China; 3Department of Infectious Diseases, The Fifth People’s Hospital of Suzhou, Suzhou, 215131, People’s Republic of China; 4Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, 430056, People’s Republic of China; 5The First Affiliated Hospital, Department of Life Science and Medicine, University of Science and Technology of China, Hefei, 230001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chuanwu Zhu; Binlian Sun, Tel/Fax +86 512 87806206 ; +86 27 84225149, Email zhuchw@126.com; binlian17@jhun.edu.cnPurpose: To investigate factors involved in T-cell depletion in combination antiretroviral therapy (cART)-treated human immunodeficiency virus 1 (HIV-1)-positive patients.Patients and Methods: 29 HIV-1-positive patients were enrolled. The CD4+, CD8+ T cell subsets and CD56dim NK cells were detected by flow cytometry. The concentrations of cytokines were measured by enzyme-linked immunosorbent assay. Extraction, amplification, and viral load quantification of specimens were performed using the Roche Cobas Ampliprep/Cobas TaqMan HIV-1 test.Results: Compared with IR group, the total number of red blood cells (RBCs) and lymphocytes (LCs) in INR group was significantly reduced, and there was a significant positive correlation between the number of RBCs and that of LCs. The overall production rates of T cells-related cytokines were lower in INR group. However, the cell-surface expression of programmed death-1 (PD-1) on CD4+ T and CD8+ T cells were markedly elevated in INR group. Moreover, it was found that the proportion and the killing ability of CD56dim NK cells significantly increased in INR patients, and significantly correlated with apoptosis of T lymphocytes.Conclusion: A poor immune reconstitution in HIV-positive patients might result from multiple factors, including bone marrow suppression, high PD-1 expression on the surface of CD4+ T cells, and over-activation of T and NK cells. Besides, the activity of NK cells and RBCs count might be important auxiliary indicators for immune reconstitution and provided a reliable guidance for developing strategies to improve immune reconstitution.Keywords: AIDS, T-cells, CD56dim NK cells, antiretroviral therapy, immune reconstitution |
| first_indexed | 2024-04-13T23:10:39Z |
| format | Article |
| id | doaj.art-4228dbfbafe645b0ac8ca48c31d35838 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-04-13T23:10:39Z |
| publishDate | 2022-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-4228dbfbafe645b0ac8ca48c31d358382022-12-22T02:25:33ZengDove Medical PressInternational Journal of General Medicine1178-70742022-05-01Volume 154575458374992CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS PatientsQian FHu SZhu YWang YLiu JQiao JShu XGao YSun BZhu CFeng Qian,1– 3,* Song Hu,4,* Yueping Zhu,2,3 Yinling Wang,2,3 Jin Liu,2,3 Jialu Qiao,4 Xiji Shu,4 Yong Gao,5 Binlian Sun,4 Chuanwu Zhu1– 3 1Department of Infectious Diseases, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People’s Republic of China; 2Department of Infectious Diseases, The Affiliated Infectious Disease Hospital of Soochow University, Suzhou, 215131, People’s Republic of China; 3Department of Infectious Diseases, The Fifth People’s Hospital of Suzhou, Suzhou, 215131, People’s Republic of China; 4Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, 430056, People’s Republic of China; 5The First Affiliated Hospital, Department of Life Science and Medicine, University of Science and Technology of China, Hefei, 230001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chuanwu Zhu; Binlian Sun, Tel/Fax +86 512 87806206 ; +86 27 84225149, Email zhuchw@126.com; binlian17@jhun.edu.cnPurpose: To investigate factors involved in T-cell depletion in combination antiretroviral therapy (cART)-treated human immunodeficiency virus 1 (HIV-1)-positive patients.Patients and Methods: 29 HIV-1-positive patients were enrolled. The CD4+, CD8+ T cell subsets and CD56dim NK cells were detected by flow cytometry. The concentrations of cytokines were measured by enzyme-linked immunosorbent assay. Extraction, amplification, and viral load quantification of specimens were performed using the Roche Cobas Ampliprep/Cobas TaqMan HIV-1 test.Results: Compared with IR group, the total number of red blood cells (RBCs) and lymphocytes (LCs) in INR group was significantly reduced, and there was a significant positive correlation between the number of RBCs and that of LCs. The overall production rates of T cells-related cytokines were lower in INR group. However, the cell-surface expression of programmed death-1 (PD-1) on CD4+ T and CD8+ T cells were markedly elevated in INR group. Moreover, it was found that the proportion and the killing ability of CD56dim NK cells significantly increased in INR patients, and significantly correlated with apoptosis of T lymphocytes.Conclusion: A poor immune reconstitution in HIV-positive patients might result from multiple factors, including bone marrow suppression, high PD-1 expression on the surface of CD4+ T cells, and over-activation of T and NK cells. Besides, the activity of NK cells and RBCs count might be important auxiliary indicators for immune reconstitution and provided a reliable guidance for developing strategies to improve immune reconstitution.Keywords: AIDS, T-cells, CD56dim NK cells, antiretroviral therapy, immune reconstitutionhttps://www.dovepress.com/cd56dim-nk-cell-is-an-important-factor-in-t-cell-depletion-of-cart-tre-peer-reviewed-fulltext-article-IJGMaidst-cellscd56dim nk cellsantiretroviral therapyimmune reconstitution |
| spellingShingle | Qian F Hu S Zhu Y Wang Y Liu J Qiao J Shu X Gao Y Sun B Zhu C CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS Patients International Journal of General Medicine aids t-cells cd56dim nk cells antiretroviral therapy immune reconstitution |
| title | CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS Patients |
| title_full | CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS Patients |
| title_fullStr | CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS Patients |
| title_full_unstemmed | CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS Patients |
| title_short | CD56dim NK Cell is an Important Factor in T Cell Depletion of cART-Treated AIDS Patients |
| title_sort | cd56dim nk cell is an important factor in t cell depletion of cart treated aids patients |
| topic | aids t-cells cd56dim nk cells antiretroviral therapy immune reconstitution |
| url | https://www.dovepress.com/cd56dim-nk-cell-is-an-important-factor-in-t-cell-depletion-of-cart-tre-peer-reviewed-fulltext-article-IJGM |
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