Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae)
We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of <i>Pieris canidia</i> by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on <i>...
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MDPI AG
2020-10-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/10/11/254 |
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| author | Ying Wang Jianqing Zhu Jie Fang Li Shen Shuojia Ma Zimiao Zhao Weidong Yu Weibin Jiang |
| author_facet | Ying Wang Jianqing Zhu Jie Fang Li Shen Shuojia Ma Zimiao Zhao Weidong Yu Weibin Jiang |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of <i>Pieris canidia</i> by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on <i>P. canidia</i> bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in <i>P. canidia</i>. These data provide an overall view of the bacterial community across the life stages and sexes in <i>P. canidia</i>. |
| first_indexed | 2024-03-10T15:20:36Z |
| format | Article |
| id | doaj.art-422b96d28507450ea20c719379b0beb1 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T15:20:36Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-422b96d28507450ea20c719379b0beb12023-11-20T18:28:46ZengMDPI AGLife2075-17292020-10-01101125410.3390/life10110254Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae)Ying Wang0Jianqing Zhu1Jie Fang2Li Shen3Shuojia Ma4Zimiao Zhao5Weidong Yu6Weibin Jiang7Laboratory of Environmental Entomology, College of Life Sciences, Shanghai Normal University, 100 Guilin Rd., Shanghai 200234, ChinaShanghai Zoological Park, 2381 Hongqiao Rd., Shanghai 200335, ChinaLaboratory of Environmental Entomology, College of Life Sciences, Shanghai Normal University, 100 Guilin Rd., Shanghai 200234, ChinaLaboratory of Environmental Entomology, College of Life Sciences, Shanghai Normal University, 100 Guilin Rd., Shanghai 200234, ChinaLaboratory of Environmental Entomology, College of Life Sciences, Shanghai Normal University, 100 Guilin Rd., Shanghai 200234, ChinaLaboratory of Environmental Entomology, College of Life Sciences, Shanghai Normal University, 100 Guilin Rd., Shanghai 200234, ChinaLaboratory of Environmental Entomology, College of Life Sciences, Shanghai Normal University, 100 Guilin Rd., Shanghai 200234, ChinaLaboratory of Environmental Entomology, College of Life Sciences, Shanghai Normal University, 100 Guilin Rd., Shanghai 200234, ChinaWe characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of <i>Pieris canidia</i> by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on <i>P. canidia</i> bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in <i>P. canidia</i>. These data provide an overall view of the bacterial community across the life stages and sexes in <i>P. canidia</i>.https://www.mdpi.com/2075-1729/10/11/254Indian cabbage whitegut microbiome16S rDNA sequencingdevelopmental stage |
| spellingShingle | Ying Wang Jianqing Zhu Jie Fang Li Shen Shuojia Ma Zimiao Zhao Weidong Yu Weibin Jiang Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae) Life Indian cabbage white gut microbiome 16S rDNA sequencing developmental stage |
| title | Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae) |
| title_full | Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae) |
| title_fullStr | Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae) |
| title_full_unstemmed | Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae) |
| title_short | Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white <em>Pieris canidia</em> (Lepidoptera: Pieridae) |
| title_sort | diversity composition and functional inference of gut microbiota in indian cabbage white em pieris canidia em lepidoptera pieridae |
| topic | Indian cabbage white gut microbiome 16S rDNA sequencing developmental stage |
| url | https://www.mdpi.com/2075-1729/10/11/254 |
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