Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism.
BACKGROUND:Gestational hypothyroidism (G-HypoT) is one of the most common thyroid diseases in pregnant women. Human milk, which closely links the mother with infant, is an important factor to the infant health. Here, we analyzed the colostrum whey proteome of women with or without G-HypoT. METHODS A...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6192644?pdf=render |
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| author | Lingli Chen Jingxuan Wang Pingping Jiang Fazheng Ren Xingen Lei Huiyuan Guo |
| author_facet | Lingli Chen Jingxuan Wang Pingping Jiang Fazheng Ren Xingen Lei Huiyuan Guo |
| author_sort | Lingli Chen |
| collection | DOAJ |
| description | BACKGROUND:Gestational hypothyroidism (G-HypoT) is one of the most common thyroid diseases in pregnant women. Human milk, which closely links the mother with infant, is an important factor to the infant health. Here, we analyzed the colostrum whey proteome of women with or without G-HypoT. METHODS AND RESULTS:Using high-mass accuracy and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS), 1055 proteins were identified. Tandem Mass Tags (TMT) analysis identified differentially expressed proteins between G-HypoT and non-G-HypoT mothers. Of 44 proteins identified, 15 proteins were significantly increased in G-HypoT colostrum whey, while 29 were significantly decreased. Analysis revealed that enzymes involved in carbohydrate metabolism, and that reflect the metabolic activities in breastfeeding women, including fructose-1, 6-bisphosphatase 1, phosphoglycerate mutase 1 were down-regulated. Cell structural proteins, biomarkers of mammary integrity development, including Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin were lower in G-HypoT colostrum whey. However, immune protein fragments like Ig gamma-3 chain C region increased in G-HypoT colostrum whey. CONCLUSION:These results implied that G-HypoT may changed human colostrum whey protein in composition level, decreasing levels of metabolic proteins and cell-structure proteins, while increasing levels of immune-related proteins, which may compromise or reflect mothers' and infants' health. |
| first_indexed | 2024-12-14T09:37:22Z |
| format | Article |
| id | doaj.art-422c342fa11c4a64b93a3e140277a43d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T09:37:22Z |
| publishDate | 2018-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-422c342fa11c4a64b93a3e140277a43d2022-12-21T23:07:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020598710.1371/journal.pone.0205987Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism.Lingli ChenJingxuan WangPingping JiangFazheng RenXingen LeiHuiyuan GuoBACKGROUND:Gestational hypothyroidism (G-HypoT) is one of the most common thyroid diseases in pregnant women. Human milk, which closely links the mother with infant, is an important factor to the infant health. Here, we analyzed the colostrum whey proteome of women with or without G-HypoT. METHODS AND RESULTS:Using high-mass accuracy and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS), 1055 proteins were identified. Tandem Mass Tags (TMT) analysis identified differentially expressed proteins between G-HypoT and non-G-HypoT mothers. Of 44 proteins identified, 15 proteins were significantly increased in G-HypoT colostrum whey, while 29 were significantly decreased. Analysis revealed that enzymes involved in carbohydrate metabolism, and that reflect the metabolic activities in breastfeeding women, including fructose-1, 6-bisphosphatase 1, phosphoglycerate mutase 1 were down-regulated. Cell structural proteins, biomarkers of mammary integrity development, including Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin were lower in G-HypoT colostrum whey. However, immune protein fragments like Ig gamma-3 chain C region increased in G-HypoT colostrum whey. CONCLUSION:These results implied that G-HypoT may changed human colostrum whey protein in composition level, decreasing levels of metabolic proteins and cell-structure proteins, while increasing levels of immune-related proteins, which may compromise or reflect mothers' and infants' health.http://europepmc.org/articles/PMC6192644?pdf=render |
| spellingShingle | Lingli Chen Jingxuan Wang Pingping Jiang Fazheng Ren Xingen Lei Huiyuan Guo Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism. PLoS ONE |
| title | Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism. |
| title_full | Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism. |
| title_fullStr | Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism. |
| title_full_unstemmed | Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism. |
| title_short | Alteration of the colostrum whey proteome in mothers with gestational hypothyroidism. |
| title_sort | alteration of the colostrum whey proteome in mothers with gestational hypothyroidism |
| url | http://europepmc.org/articles/PMC6192644?pdf=render |
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