Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in Poplar
<i>Populus</i> is a genus of globally significant plantation trees used widely in industrial and agricultural production. Poplars are easily damaged by <i>Micromelalopha troglodyta</i> and <i>Hyphantria cunea</i>, resulting in decreasing quality. <i>Bt</i...
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MDPI AG
2022-11-01
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| Series: | Life |
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| author | Ali Movahedi Hui Wei Abdul Razak Alhassan Raphael Dzinyela Pu Wang Weibo Sun Qiang Zhuge Chen Xu |
| author_facet | Ali Movahedi Hui Wei Abdul Razak Alhassan Raphael Dzinyela Pu Wang Weibo Sun Qiang Zhuge Chen Xu |
| author_sort | Ali Movahedi |
| collection | DOAJ |
| description | <i>Populus</i> is a genus of globally significant plantation trees used widely in industrial and agricultural production. Poplars are easily damaged by <i>Micromelalopha troglodyta</i> and <i>Hyphantria cunea</i>, resulting in decreasing quality. <i>Bt</i> toxin-encoded by the <i>Cry</i> gene has been widely adopted in poplar breeding because of its strong insect resistance. There is still no comprehensive and sufficient information about the effects of <i>Cry1Ah1</i>-modified (CM) poplars on the ecological environment. Here, we sampled the rhizosphere soils of field-grown CM and non-transgenic (NT) poplars and applied 16S rRNA and internal transcribed spacer amplicon Illumina MiSeq sequencing to determine the bacterial community associated with the CM and NT poplars. Based on the high-throughput sequencing of samples, we found that the predominant taxa included <i>Proteobacteria</i> (about 40% of the total bacteria), <i>Acidobacteria</i> (about 20% of the total bacteria), and <i>Actinobacteria</i> (about 20% of the total bacteria) collected from the natural rhizosphere of NT and CM poplars. In addition, studies on the microbial diversity of poplar showed that <i>Cry1Ah1</i> expression has no significant influence on rhizosphere soil alkaline nitrogen, but significantly affects soil phosphorus, soil microbial biomass nitrogen, and carbon. The results exhibited a similar bacterial community structure between CM varieties affected by the expression of <i>Cry1Ah1</i> and non-transgenic poplars. In addition, <i>Cry1Ah1</i> expression revealed no significant influence on the composition of rhizosphere microbiomes. These results broadly reflect the effect of the <i>Bt</i> toxin-encoded by <i>Cry1Ah1</i> on the ecology and environment and provide a clear path for researchers to continue research in this field in the future. |
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| language | English |
| last_indexed | 2024-03-09T18:54:40Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-422c5ce575954fe6a7fded3751490d742023-11-24T05:31:38ZengMDPI AGLife2075-17292022-11-011211183010.3390/life12111830Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in PoplarAli Movahedi0Hui Wei1Abdul Razak Alhassan2Raphael Dzinyela3Pu Wang4Weibo Sun5Qiang Zhuge6Chen Xu7College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong 226019, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaNanjing Key Laboratory of Quality and Safety of Agricultural Product, Nanjing Xiaozhuang University, Nanjing 211171, China<i>Populus</i> is a genus of globally significant plantation trees used widely in industrial and agricultural production. Poplars are easily damaged by <i>Micromelalopha troglodyta</i> and <i>Hyphantria cunea</i>, resulting in decreasing quality. <i>Bt</i> toxin-encoded by the <i>Cry</i> gene has been widely adopted in poplar breeding because of its strong insect resistance. There is still no comprehensive and sufficient information about the effects of <i>Cry1Ah1</i>-modified (CM) poplars on the ecological environment. Here, we sampled the rhizosphere soils of field-grown CM and non-transgenic (NT) poplars and applied 16S rRNA and internal transcribed spacer amplicon Illumina MiSeq sequencing to determine the bacterial community associated with the CM and NT poplars. Based on the high-throughput sequencing of samples, we found that the predominant taxa included <i>Proteobacteria</i> (about 40% of the total bacteria), <i>Acidobacteria</i> (about 20% of the total bacteria), and <i>Actinobacteria</i> (about 20% of the total bacteria) collected from the natural rhizosphere of NT and CM poplars. In addition, studies on the microbial diversity of poplar showed that <i>Cry1Ah1</i> expression has no significant influence on rhizosphere soil alkaline nitrogen, but significantly affects soil phosphorus, soil microbial biomass nitrogen, and carbon. The results exhibited a similar bacterial community structure between CM varieties affected by the expression of <i>Cry1Ah1</i> and non-transgenic poplars. In addition, <i>Cry1Ah1</i> expression revealed no significant influence on the composition of rhizosphere microbiomes. These results broadly reflect the effect of the <i>Bt</i> toxin-encoded by <i>Cry1Ah1</i> on the ecology and environment and provide a clear path for researchers to continue research in this field in the future.https://www.mdpi.com/2075-1729/12/11/1830<i>Bt</i> toxins<i>Cry1Ah1</i> transgenic poplarecologyenvironmentrhizosphere |
| spellingShingle | Ali Movahedi Hui Wei Abdul Razak Alhassan Raphael Dzinyela Pu Wang Weibo Sun Qiang Zhuge Chen Xu Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in Poplar Life <i>Bt</i> toxins <i>Cry1Ah1</i> transgenic poplar ecology environment rhizosphere |
| title | Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in Poplar |
| title_full | Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in Poplar |
| title_fullStr | Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in Poplar |
| title_full_unstemmed | Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in Poplar |
| title_short | Evaluation of the Ecological Environment Affected by <i>Cry1Ah1</i> in Poplar |
| title_sort | evaluation of the ecological environment affected by i cry1ah1 i in poplar |
| topic | <i>Bt</i> toxins <i>Cry1Ah1</i> transgenic poplar ecology environment rhizosphere |
| url | https://www.mdpi.com/2075-1729/12/11/1830 |
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