Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran
Introduction: Biofilm producing Staphylococcus aureus is known as one of the major causative agents of infections, failure of implanted devices and persistent infection among hospitalized patients. The aim of the present study was to determine the frequency of biofilm producing S. aureus isolates am...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-03-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | https://jmb.tums.ac.ir/index.php/jmb/article/view/173 |
| _version_ | 1818807516949118976 |
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| author | Reza Khashei Hadi Sedigh Ebrahim-Saraie Mohammad Motamedifar Mehrdad Zalipour Jamal Sarvari |
| author_facet | Reza Khashei Hadi Sedigh Ebrahim-Saraie Mohammad Motamedifar Mehrdad Zalipour Jamal Sarvari |
| author_sort | Reza Khashei |
| collection | DOAJ |
| description | Introduction: Biofilm producing Staphylococcus aureus is known as one of the major
causative agents of infections, failure of implanted devices and persistent infection
among hospitalized patients. The aim of the present study was to determine the
frequency of biofilm producing S. aureus isolates amongst the clinical specimens.
Methods: This cross-sectional study was conducted during 2012 to 2013 in two
teaching hospitals in Shiraz, southwest of Iran. Totally, 345 S. aureus isolates from
various clinical specimens were included. Biofilm producing isolates were
phenotypically detected using Congo Red Agar (CRA) and genotypically by PCR
assay for the icaA and icaD genes.
Results: Of the 345 S. aureus isolates, 42.3% were methicillin-resistant S. aureus
(MRSA) and subsequently 57.7% were methicillin susceptible isolates. The results of
CRA plates showed that 77 (52.7%) and 74 (37.2%) of MRSA and MSSA were
biofilm producing isolates. The frequency of icaA/D genes among MRSA and MSSA
isolates was 127 (87%) and 167 (83.9%), respectively.
Conclusion: Such a high rate of icaA/D harboring S. aureus among clinical isolates
suggest the risk for establishing persistent infections in the hospital settings. |
| first_indexed | 2024-12-18T19:26:55Z |
| format | Article |
| id | doaj.art-422cebb064a54f23b86919b745ec486e |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-12-18T19:26:55Z |
| publishDate | 2015-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-422cebb064a54f23b86919b745ec486e2022-12-21T20:55:50ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812015-03-0141-2Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, IranReza Khashei0Hadi Sedigh Ebrahim-Saraie1Mohammad Motamedifar2Mehrdad Zalipour3Jamal Sarvari4Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran Shiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.Introduction: Biofilm producing Staphylococcus aureus is known as one of the major causative agents of infections, failure of implanted devices and persistent infection among hospitalized patients. The aim of the present study was to determine the frequency of biofilm producing S. aureus isolates amongst the clinical specimens. Methods: This cross-sectional study was conducted during 2012 to 2013 in two teaching hospitals in Shiraz, southwest of Iran. Totally, 345 S. aureus isolates from various clinical specimens were included. Biofilm producing isolates were phenotypically detected using Congo Red Agar (CRA) and genotypically by PCR assay for the icaA and icaD genes. Results: Of the 345 S. aureus isolates, 42.3% were methicillin-resistant S. aureus (MRSA) and subsequently 57.7% were methicillin susceptible isolates. The results of CRA plates showed that 77 (52.7%) and 74 (37.2%) of MRSA and MSSA were biofilm producing isolates. The frequency of icaA/D genes among MRSA and MSSA isolates was 127 (87%) and 167 (83.9%), respectively. Conclusion: Such a high rate of icaA/D harboring S. aureus among clinical isolates suggest the risk for establishing persistent infections in the hospital settings.https://jmb.tums.ac.ir/index.php/jmb/article/view/173BiofilmStaphylococcus aureusCongo red agaricaAicaD |
| spellingShingle | Reza Khashei Hadi Sedigh Ebrahim-Saraie Mohammad Motamedifar Mehrdad Zalipour Jamal Sarvari Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran Journal of Medical Bacteriology Biofilm Staphylococcus aureus Congo red agar icaA icaD |
| title | Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran |
| title_full | Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran |
| title_fullStr | Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran |
| title_full_unstemmed | Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran |
| title_short | Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran |
| title_sort | detection of icaa icad genes and biofilm formation among clinical isolates of staphylococcus aureus from shiraz iran |
| topic | Biofilm Staphylococcus aureus Congo red agar icaA icaD |
| url | https://jmb.tums.ac.ir/index.php/jmb/article/view/173 |
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