The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo
Early postnatal events are important for the development of the neonatal immune system. Harboring the pioneering microorganisms forming the microbiota of the neonatal gastrointestinal tract is important for priming the immune system, as well as inducing appropriate tolerance to the relatively innocu...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/10/1624 |
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| author | Lenka Súkeníková Viktor Černý Jan Věcek Petra Petrásková Olga Novotná Šimon Vobruba Tereza Michalčíková Jan Procházka Libuše Kolářová Ludmila Prokešová Jiří Hrdý |
| author_facet | Lenka Súkeníková Viktor Černý Jan Věcek Petra Petrásková Olga Novotná Šimon Vobruba Tereza Michalčíková Jan Procházka Libuše Kolářová Ludmila Prokešová Jiří Hrdý |
| author_sort | Lenka Súkeníková |
| collection | DOAJ |
| description | Early postnatal events are important for the development of the neonatal immune system. Harboring the pioneering microorganisms forming the microbiota of the neonatal gastrointestinal tract is important for priming the immune system, as well as inducing appropriate tolerance to the relatively innocuous environmental antigens and compounds of normal healthy microbiota. Early postnatal supplementation of suitable, safe probiotics could accelerate this process. In the current study, the immunomodulatory capacity of the probiotic strain of <i>Escherichia coli</i> O83:K24:H31 (EcO83) was characterized in vitro and in vivo. We compared the capacity of EcO83 with and without hemolytic activity on selected immune characteristics in vitro as determined by flow cytometry and quantitative real-time PCR. Both strains with and without hemolytic activity exerted comparable capacity on the maturation of dendritic cells while preserving the induction of interleukin 10 (<i>Il10</i>) expression in dendritic cells and T cells cocultured with EcO83 primed dendritic cells. Early postnatal supplementation with EcO83 led to massive but transient colonization of the neonatal gastrointestinal tract, as detected by in vivo bioimaging. Early postnatal EcO83 administration promoted gut barrier function by increasing the expression of claudin and occludin and the expression of <i>Il10</i>. Early postnatal EcO83 application promotes maturation of the neonatal immune system and promotes immunoregulatory and gut barrier functions. |
| first_indexed | 2024-03-10T03:10:30Z |
| format | Article |
| id | doaj.art-422e4d098477422cb8b4763c68befb2d |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:10:30Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-422e4d098477422cb8b4763c68befb2d2023-11-23T10:27:18ZengMDPI AGCells2073-44092022-05-011110162410.3390/cells11101624The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In VivoLenka Súkeníková0Viktor Černý1Jan Věcek2Petra Petrásková3Olga Novotná4Šimon Vobruba5Tereza Michalčíková6Jan Procházka7Libuše Kolářová8Ludmila Prokešová9Jiří Hrdý10First Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicFirst Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicFirst Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicFirst Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicFirst Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicCzech Academy of Sciences, 142 20 Prague, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech RepublicFirst Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicFirst Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicFirst Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicEarly postnatal events are important for the development of the neonatal immune system. Harboring the pioneering microorganisms forming the microbiota of the neonatal gastrointestinal tract is important for priming the immune system, as well as inducing appropriate tolerance to the relatively innocuous environmental antigens and compounds of normal healthy microbiota. Early postnatal supplementation of suitable, safe probiotics could accelerate this process. In the current study, the immunomodulatory capacity of the probiotic strain of <i>Escherichia coli</i> O83:K24:H31 (EcO83) was characterized in vitro and in vivo. We compared the capacity of EcO83 with and without hemolytic activity on selected immune characteristics in vitro as determined by flow cytometry and quantitative real-time PCR. Both strains with and without hemolytic activity exerted comparable capacity on the maturation of dendritic cells while preserving the induction of interleukin 10 (<i>Il10</i>) expression in dendritic cells and T cells cocultured with EcO83 primed dendritic cells. Early postnatal supplementation with EcO83 led to massive but transient colonization of the neonatal gastrointestinal tract, as detected by in vivo bioimaging. Early postnatal EcO83 administration promoted gut barrier function by increasing the expression of claudin and occludin and the expression of <i>Il10</i>. Early postnatal EcO83 application promotes maturation of the neonatal immune system and promotes immunoregulatory and gut barrier functions.https://www.mdpi.com/2073-4409/11/10/1624probiotic<i>E. coli</i> O83:K24:H31luciferasedendritic cellIL-10indol amine 2,3 dioxygenase |
| spellingShingle | Lenka Súkeníková Viktor Černý Jan Věcek Petra Petrásková Olga Novotná Šimon Vobruba Tereza Michalčíková Jan Procházka Libuše Kolářová Ludmila Prokešová Jiří Hrdý The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo Cells probiotic <i>E. coli</i> O83:K24:H31 luciferase dendritic cell IL-10 indol amine 2,3 dioxygenase |
| title | The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo |
| title_full | The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo |
| title_fullStr | The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo |
| title_full_unstemmed | The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo |
| title_short | The Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo |
| title_sort | impact of i escherichia coli i probiotic strain o83 k24 h31 on the maturation of dendritic cells and immunoregulatory functions in vitro and in vivo |
| topic | probiotic <i>E. coli</i> O83:K24:H31 luciferase dendritic cell IL-10 indol amine 2,3 dioxygenase |
| url | https://www.mdpi.com/2073-4409/11/10/1624 |
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