Immune response to respiratory syncytial virus in young Brazilian children
We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were test...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2002-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011 |
| _version_ | 1818366020956454912 |
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| author | D.A.O. Queiróz E.L. Durigon V.F. Botosso B. Ejzemberg S.E. Vieira J.R. Mineo C. Yamashita N. Hein C.L. Lopes A.L. Cacharo K.E. Stewien |
| author_facet | D.A.O. Queiróz E.L. Durigon V.F. Botosso B. Ejzemberg S.E. Vieira J.R. Mineo C. Yamashita N. Hein C.L. Lopes A.L. Cacharo K.E. Stewien |
| author_sort | D.A.O. Queiróz |
| collection | DOAJ |
| description | We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV. |
| first_indexed | 2024-12-13T22:29:31Z |
| format | Article |
| id | doaj.art-42325c5bd266408b8ee0a13673095f44 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-13T22:29:31Z |
| publishDate | 2002-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-42325c5bd266408b8ee0a13673095f442022-12-21T23:29:07ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2002-10-0135101183119310.1590/S0100-879X2002001000011Immune response to respiratory syncytial virus in young Brazilian childrenD.A.O. QueirózE.L. DurigonV.F. BotossoB. EjzembergS.E. VieiraJ.R. MineoC. YamashitaN. HeinC.L. LopesA.L. CacharoK.E. StewienWe have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011Respiratory syncytial virusPeripheral blood mononuclear cellsYoung childrenSurface antigensCytokines |
| spellingShingle | D.A.O. Queiróz E.L. Durigon V.F. Botosso B. Ejzemberg S.E. Vieira J.R. Mineo C. Yamashita N. Hein C.L. Lopes A.L. Cacharo K.E. Stewien Immune response to respiratory syncytial virus in young Brazilian children Brazilian Journal of Medical and Biological Research Respiratory syncytial virus Peripheral blood mononuclear cells Young children Surface antigens Cytokines |
| title | Immune response to respiratory syncytial virus in young Brazilian children |
| title_full | Immune response to respiratory syncytial virus in young Brazilian children |
| title_fullStr | Immune response to respiratory syncytial virus in young Brazilian children |
| title_full_unstemmed | Immune response to respiratory syncytial virus in young Brazilian children |
| title_short | Immune response to respiratory syncytial virus in young Brazilian children |
| title_sort | immune response to respiratory syncytial virus in young brazilian children |
| topic | Respiratory syncytial virus Peripheral blood mononuclear cells Young children Surface antigens Cytokines |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011 |
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