Non‐additive effects between genotypes: Implications for competitive fitness assays
Abstract Competitive fitness assays are widely used in evolutionary biology and typically rely on a reference strain to compare different focal genotypes. This approach implicitly relies on the absence of interaction between the competing genotypes. In other words, the performance of the reference s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-11-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.10713 |
| _version_ | 1797448662347939840 |
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| author | Changyi Xiao Sara Duarri‐Redondo Dagny A. V. Thorhölludottir Yiwen Chen Christian Schlötterer |
| author_facet | Changyi Xiao Sara Duarri‐Redondo Dagny A. V. Thorhölludottir Yiwen Chen Christian Schlötterer |
| author_sort | Changyi Xiao |
| collection | DOAJ |
| description | Abstract Competitive fitness assays are widely used in evolutionary biology and typically rely on a reference strain to compare different focal genotypes. This approach implicitly relies on the absence of interaction between the competing genotypes. In other words, the performance of the reference strain must not depend on the competitor. This report scrutinized this assumption by competing diverged Drosophila simulans populations against a common reference strain. We detected strong evidence for interaction between the competing genotypes: (1) Frequency‐dependent selection was common with opposite effects in genetically diverged populations. (2) Temporal heterogeneity of fitness estimates, which can be partially attributed to a competitor‐specific delay in the eclosion of the reference strain. We propose that this inconsistent behavior of the reference strain can be considered a specific case of a genotype × environment interaction. Focal populations could modify the environment of the reference strain, either indirectly by altering the microbiome composition and food availability or directly by genotype‐specific cannibalism. Our results provide new insights into the interaction of diverged genotypes and have important implications for the interpretation of competitive fitness assays. |
| first_indexed | 2024-03-09T14:13:37Z |
| format | Article |
| id | doaj.art-42339d3c33874f3dbb96419c62c60ec8 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-03-09T14:13:37Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-42339d3c33874f3dbb96419c62c60ec82023-11-29T05:44:08ZengWileyEcology and Evolution2045-77582023-11-011311n/an/a10.1002/ece3.10713Non‐additive effects between genotypes: Implications for competitive fitness assaysChangyi Xiao0Sara Duarri‐Redondo1Dagny A. V. Thorhölludottir2Yiwen Chen3Christian Schlötterer4Institut für Populationsgenetik Vetmeduni Vienna AustriaInstitut für Populationsgenetik Vetmeduni Vienna AustriaInstitut für Populationsgenetik Vetmeduni Vienna AustriaInstitut für Populationsgenetik Vetmeduni Vienna AustriaInstitut für Populationsgenetik Vetmeduni Vienna AustriaAbstract Competitive fitness assays are widely used in evolutionary biology and typically rely on a reference strain to compare different focal genotypes. This approach implicitly relies on the absence of interaction between the competing genotypes. In other words, the performance of the reference strain must not depend on the competitor. This report scrutinized this assumption by competing diverged Drosophila simulans populations against a common reference strain. We detected strong evidence for interaction between the competing genotypes: (1) Frequency‐dependent selection was common with opposite effects in genetically diverged populations. (2) Temporal heterogeneity of fitness estimates, which can be partially attributed to a competitor‐specific delay in the eclosion of the reference strain. We propose that this inconsistent behavior of the reference strain can be considered a specific case of a genotype × environment interaction. Focal populations could modify the environment of the reference strain, either indirectly by altering the microbiome composition and food availability or directly by genotype‐specific cannibalism. Our results provide new insights into the interaction of diverged genotypes and have important implications for the interpretation of competitive fitness assays.https://doi.org/10.1002/ece3.10713competitive fitnessDrosophilaexperimental evolutionfrequency‐dependent selection |
| spellingShingle | Changyi Xiao Sara Duarri‐Redondo Dagny A. V. Thorhölludottir Yiwen Chen Christian Schlötterer Non‐additive effects between genotypes: Implications for competitive fitness assays Ecology and Evolution competitive fitness Drosophila experimental evolution frequency‐dependent selection |
| title | Non‐additive effects between genotypes: Implications for competitive fitness assays |
| title_full | Non‐additive effects between genotypes: Implications for competitive fitness assays |
| title_fullStr | Non‐additive effects between genotypes: Implications for competitive fitness assays |
| title_full_unstemmed | Non‐additive effects between genotypes: Implications for competitive fitness assays |
| title_short | Non‐additive effects between genotypes: Implications for competitive fitness assays |
| title_sort | non additive effects between genotypes implications for competitive fitness assays |
| topic | competitive fitness Drosophila experimental evolution frequency‐dependent selection |
| url | https://doi.org/10.1002/ece3.10713 |
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