Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells
In this work the physicochemical and biological properties of nanocrystalline TiO2 thin films were investigated. Thin films were prepared by magnetron sputtering method. Their properties were examined by X-ray diffraction, photoelectron spectroscopy, atomic force microscopy, optical transmission met...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2015-09-01
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| Series: | Polish Journal of Chemical Technology |
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| Online Access: | https://doi.org/10.1515/pjct-2015-0047 |
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| author | Osękowska Małgorzata Karuga-Kuźniewska Ewa Wojcieszak Damian Mazur Michał Poniedziałek Agata Kaczmarek Danuta Szymonowicz Maria Rybak Zbigniew |
| author_facet | Osękowska Małgorzata Karuga-Kuźniewska Ewa Wojcieszak Damian Mazur Michał Poniedziałek Agata Kaczmarek Danuta Szymonowicz Maria Rybak Zbigniew |
| author_sort | Osękowska Małgorzata |
| collection | DOAJ |
| description | In this work the physicochemical and biological properties of nanocrystalline TiO2 thin films were investigated. Thin films were prepared by magnetron sputtering method. Their properties were examined by X-ray diffraction, photoelectron spectroscopy, atomic force microscopy, optical transmission method and optical profiler. Moreover, surface wettability and scratch resistance were determined. It was found that as-deposited coatings were nanocrystalline and had TiO2-anatase structure, built from crystallites in size of 24 nm. The surface of the films was homogenous, composed of closely packed grains and hydrophilic. Due to nanocrystalline structure thin films exhibited good scratch resistance. The results were correlated to the biological activity (in vitro) of thin films. Morphological changes of mouse fibroblasts (L929 cell line) after contact with the surface of TiO2 films were evaluated with the use of a contrast-phase microscope, while their viability was tested by MTT colorimetric assay. The viability of cell line upon contact with the surface of nanocrystalline TiO2 film was comparable to the control sample. L929 cells had homogenous cytoplasm and were forming a confluent monofilm, while lysis and inhibition of cell growth was not observed. Moreover, the viability in contact with surface of examined films was high. This confirms non-cytotoxic effect of TiO2 film surface on mouse fibroblasts. |
| first_indexed | 2024-12-17T12:07:10Z |
| format | Article |
| id | doaj.art-42348a9be47d4840b70cd288ecd16cb8 |
| institution | Directory Open Access Journal |
| issn | 1899-4741 |
| language | English |
| last_indexed | 2024-12-17T12:07:10Z |
| publishDate | 2015-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Chemical Technology |
| spelling | doaj.art-42348a9be47d4840b70cd288ecd16cb82022-12-21T21:49:34ZengSciendoPolish Journal of Chemical Technology1899-47412015-09-01173333910.1515/pjct-2015-0047pjct-2015-0047Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cellsOsękowska Małgorzata0Karuga-Kuźniewska Ewa1Wojcieszak Damian2Mazur Michał3Poniedziałek Agata4Kaczmarek Danuta5Szymonowicz Maria6Rybak Zbigniew7 Wroclaw Medical University, Department for Experimental Surgery and Biomaterials Research, Poland Wroclaw Medical University, Department for Experimental Surgery and Biomaterials Research, Poland Wroclaw University of Technology, Faculty of Microsystem Electronics and Photonics, Wroclaw, Poland Wroclaw University of Technology, Faculty of Microsystem Electronics and Photonics, Wroclaw, Poland Wroclaw University of Technology, Faculty of Microsystem Electronics and Photonics, Wroclaw, Poland Wroclaw University of Technology, Faculty of Microsystem Electronics and Photonics, Wroclaw, Poland Wroclaw Medical University, Department for Experimental Surgery and Biomaterials Research, Poland Wroclaw Medical University, Department for Experimental Surgery and Biomaterials Research, PolandIn this work the physicochemical and biological properties of nanocrystalline TiO2 thin films were investigated. Thin films were prepared by magnetron sputtering method. Their properties were examined by X-ray diffraction, photoelectron spectroscopy, atomic force microscopy, optical transmission method and optical profiler. Moreover, surface wettability and scratch resistance were determined. It was found that as-deposited coatings were nanocrystalline and had TiO2-anatase structure, built from crystallites in size of 24 nm. The surface of the films was homogenous, composed of closely packed grains and hydrophilic. Due to nanocrystalline structure thin films exhibited good scratch resistance. The results were correlated to the biological activity (in vitro) of thin films. Morphological changes of mouse fibroblasts (L929 cell line) after contact with the surface of TiO2 films were evaluated with the use of a contrast-phase microscope, while their viability was tested by MTT colorimetric assay. The viability of cell line upon contact with the surface of nanocrystalline TiO2 film was comparable to the control sample. L929 cells had homogenous cytoplasm and were forming a confluent monofilm, while lysis and inhibition of cell growth was not observed. Moreover, the viability in contact with surface of examined films was high. This confirms non-cytotoxic effect of TiO2 film surface on mouse fibroblasts.https://doi.org/10.1515/pjct-2015-0047tio2surface propertiesnanocrystalline thin filmcell morphologyviability of l929 cells |
| spellingShingle | Osękowska Małgorzata Karuga-Kuźniewska Ewa Wojcieszak Damian Mazur Michał Poniedziałek Agata Kaczmarek Danuta Szymonowicz Maria Rybak Zbigniew Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells Polish Journal of Chemical Technology tio2 surface properties nanocrystalline thin film cell morphology viability of l929 cells |
| title | Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells |
| title_full | Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells |
| title_fullStr | Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells |
| title_full_unstemmed | Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells |
| title_short | Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells |
| title_sort | influence of nanocrystalline structure and surface properties of tio2 thin films on the viability of l929 cells |
| topic | tio2 surface properties nanocrystalline thin film cell morphology viability of l929 cells |
| url | https://doi.org/10.1515/pjct-2015-0047 |
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