Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain
Understanding amazingly complex brain functions and pathologies requires a complete cerebral vascular atlas in stereotaxic coordinates. Making a precise atlas for cerebral arteries and veins has been a century-old objective in neuroscience and neuropathology. Using micro-optical sectioning tomograph...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-12-01
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| Series: | Frontiers in Neuroanatomy |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fnana.2017.00128/full |
| _version_ | 1818433299091030016 |
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| author | Benyi Xiong Benyi Xiong Anan Li Anan Li Yang Lou Yang Lou Shangbin Chen Shangbin Chen Ben Long Ben Long Jie Peng Jie Peng Zhongqin Yang Zhongqin Yang Tonghui Xu Tonghui Xu Xiaoquan Yang Xiaoquan Yang Xiangning Li Xiangning Li Tao Jiang Tao Jiang Qingming Luo Qingming Luo Hui Gong Hui Gong |
| author_facet | Benyi Xiong Benyi Xiong Anan Li Anan Li Yang Lou Yang Lou Shangbin Chen Shangbin Chen Ben Long Ben Long Jie Peng Jie Peng Zhongqin Yang Zhongqin Yang Tonghui Xu Tonghui Xu Xiaoquan Yang Xiaoquan Yang Xiangning Li Xiangning Li Tao Jiang Tao Jiang Qingming Luo Qingming Luo Hui Gong Hui Gong |
| author_sort | Benyi Xiong |
| collection | DOAJ |
| description | Understanding amazingly complex brain functions and pathologies requires a complete cerebral vascular atlas in stereotaxic coordinates. Making a precise atlas for cerebral arteries and veins has been a century-old objective in neuroscience and neuropathology. Using micro-optical sectioning tomography (MOST) with a modified Nissl staining method, we acquired five mouse brain data sets containing arteries, veins, and microvessels. Based on the brain-wide vascular spatial structures and brain regions indicated by cytoarchitecture in one and the same mouse brain, we reconstructed and annotated the vascular system atlas of both arteries and veins of the whole mouse brain for the first time. The distributing patterns of the vascular system within the brain regions were acquired and our results show that the patterns of individual vessels are different from each other. Reconstruction and statistical analysis of the microvascular network, including derivation of quantitative vascular densities, indicate significant differences mainly in vessels with diameters less than 8 μm and large than 20 μm across different brain regions. Our precise cerebral vascular atlas provides an important resource and approach for quantitative studies of brain functions and diseases. |
| first_indexed | 2024-12-14T16:18:53Z |
| format | Article |
| id | doaj.art-423522eaa27740c3800313cd1586a32e |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-12-14T16:18:53Z |
| publishDate | 2017-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-423522eaa27740c3800313cd1586a32e2022-12-21T22:54:51ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292017-12-011110.3389/fnana.2017.00128303118Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse BrainBenyi Xiong0Benyi Xiong1Anan Li2Anan Li3Yang Lou4Yang Lou5Shangbin Chen6Shangbin Chen7Ben Long8Ben Long9Jie Peng10Jie Peng11Zhongqin Yang12Zhongqin Yang13Tonghui Xu14Tonghui Xu15Xiaoquan Yang16Xiaoquan Yang17Xiangning Li18Xiangning Li19Tao Jiang20Tao Jiang21Qingming Luo22Qingming Luo23Hui Gong24Hui Gong25Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaUnderstanding amazingly complex brain functions and pathologies requires a complete cerebral vascular atlas in stereotaxic coordinates. Making a precise atlas for cerebral arteries and veins has been a century-old objective in neuroscience and neuropathology. Using micro-optical sectioning tomography (MOST) with a modified Nissl staining method, we acquired five mouse brain data sets containing arteries, veins, and microvessels. Based on the brain-wide vascular spatial structures and brain regions indicated by cytoarchitecture in one and the same mouse brain, we reconstructed and annotated the vascular system atlas of both arteries and veins of the whole mouse brain for the first time. The distributing patterns of the vascular system within the brain regions were acquired and our results show that the patterns of individual vessels are different from each other. Reconstruction and statistical analysis of the microvascular network, including derivation of quantitative vascular densities, indicate significant differences mainly in vessels with diameters less than 8 μm and large than 20 μm across different brain regions. Our precise cerebral vascular atlas provides an important resource and approach for quantitative studies of brain functions and diseases.http://journal.frontiersin.org/article/10.3389/fnana.2017.00128/fullwhole mouse brainthree-dimensional reconstructionfine vascular atlasvascular distributing patternsmicrovesselsquantitative calculation |
| spellingShingle | Benyi Xiong Benyi Xiong Anan Li Anan Li Yang Lou Yang Lou Shangbin Chen Shangbin Chen Ben Long Ben Long Jie Peng Jie Peng Zhongqin Yang Zhongqin Yang Tonghui Xu Tonghui Xu Xiaoquan Yang Xiaoquan Yang Xiangning Li Xiangning Li Tao Jiang Tao Jiang Qingming Luo Qingming Luo Hui Gong Hui Gong Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain Frontiers in Neuroanatomy whole mouse brain three-dimensional reconstruction fine vascular atlas vascular distributing patterns microvessels quantitative calculation |
| title | Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain |
| title_full | Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain |
| title_fullStr | Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain |
| title_full_unstemmed | Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain |
| title_short | Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain |
| title_sort | precise cerebral vascular atlas in stereotaxic coordinates of whole mouse brain |
| topic | whole mouse brain three-dimensional reconstruction fine vascular atlas vascular distributing patterns microvessels quantitative calculation |
| url | http://journal.frontiersin.org/article/10.3389/fnana.2017.00128/full |
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