Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocols
ABSTRACT Objective: The aim of this study was to evaluate bone microdamage in sites prepared for implant placement by using an ex vivo model with three drilling rotation speeds. Methods: Bovine bone ribs were used for the creation of 18 osteotomy sites at different rotation speeds: 1200 rpm, 800 r...
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| Format: | Article |
| Language: | English |
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Faculdade São Leopoldo Mandic
2022-11-01
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| Series: | RGO: Revista Gaúcha de Odontologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372022000100325&tlng=en |
| _version_ | 1827913878680371200 |
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| author | Isabella de Almeida FRANCISQUINI Gustavo Davi RABELO Neuza Maria Souza Picorelli ASSIS Bruno Salles SOTTO-MAIOR Pedro Henrique Justino Oliveira LIMIRIO Karina Lopes DEVITO |
| author_facet | Isabella de Almeida FRANCISQUINI Gustavo Davi RABELO Neuza Maria Souza Picorelli ASSIS Bruno Salles SOTTO-MAIOR Pedro Henrique Justino Oliveira LIMIRIO Karina Lopes DEVITO |
| author_sort | Isabella de Almeida FRANCISQUINI |
| collection | DOAJ |
| description | ABSTRACT Objective: The aim of this study was to evaluate bone microdamage in sites prepared for implant placement by using an ex vivo model with three drilling rotation speeds. Methods: Bovine bone ribs were used for the creation of 18 osteotomy sites at different rotation speeds: 1200 rpm, 800 rpm, and 400 rpm. Specimens were stained with xylenol orange and prepared for histological analysis by using fluorescence and polarized light microscopies. Bone microdamage was evaluated by number and based on total bone area, as follows: microfracture density (Fr.D = n/mm2), microcrack morphology (diffuse or linear), and density (Cr.D = n/mm2), and presence of bone chips. To complement the analysis, linear microcracks were assessed by using confocal microscopy for three-dimensional visualization. Results: Bone microdamage on the osteotomy sites included microcracks, diffuse damages, microfracture, and bone chip formation. There was an association between bone microdamage and cancellous bone (p 0.0016), as well as a positive correlation between Fr.D and Cr.D (p 0.05, r 0.54). BM occurrence was not different between the three rotation speeds. In 3D, the height of the microcrack depth was 60.81 µm. Conclusion: Bone microdamage occurs during osteotomy, and the ex vivo model used was effective for the assessment of these biomechanical parameters. In addition, microdamage was not influenced by the drilling rotation speed in this experimental condition. |
| first_indexed | 2024-03-13T02:37:13Z |
| format | Article |
| id | doaj.art-423643138ee44684bda108619b042d49 |
| institution | Directory Open Access Journal |
| issn | 1981-8637 |
| language | English |
| last_indexed | 2024-03-13T02:37:13Z |
| publishDate | 2022-11-01 |
| publisher | Faculdade São Leopoldo Mandic |
| record_format | Article |
| series | RGO: Revista Gaúcha de Odontologia |
| spelling | doaj.art-423643138ee44684bda108619b042d492023-06-28T23:16:16ZengFaculdade São Leopoldo MandicRGO: Revista Gaúcha de Odontologia1981-86372022-11-017010.1590/1981-86372022005320210090Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocolsIsabella de Almeida FRANCISQUINIhttps://orcid.org/0000-0001-9906-2145Gustavo Davi RABELOhttps://orcid.org/0000-0001-9511-5078Neuza Maria Souza Picorelli ASSIShttps://orcid.org/0000-0002-8612-5044Bruno Salles SOTTO-MAIORhttps://orcid.org/0000-0002-9462-0299Pedro Henrique Justino Oliveira LIMIRIOhttps://orcid.org/0000-0002-0089-3772Karina Lopes DEVITOhttps://orcid.org/0000-0001-5037-5466ABSTRACT Objective: The aim of this study was to evaluate bone microdamage in sites prepared for implant placement by using an ex vivo model with three drilling rotation speeds. Methods: Bovine bone ribs were used for the creation of 18 osteotomy sites at different rotation speeds: 1200 rpm, 800 rpm, and 400 rpm. Specimens were stained with xylenol orange and prepared for histological analysis by using fluorescence and polarized light microscopies. Bone microdamage was evaluated by number and based on total bone area, as follows: microfracture density (Fr.D = n/mm2), microcrack morphology (diffuse or linear), and density (Cr.D = n/mm2), and presence of bone chips. To complement the analysis, linear microcracks were assessed by using confocal microscopy for three-dimensional visualization. Results: Bone microdamage on the osteotomy sites included microcracks, diffuse damages, microfracture, and bone chip formation. There was an association between bone microdamage and cancellous bone (p 0.0016), as well as a positive correlation between Fr.D and Cr.D (p 0.05, r 0.54). BM occurrence was not different between the three rotation speeds. In 3D, the height of the microcrack depth was 60.81 µm. Conclusion: Bone microdamage occurs during osteotomy, and the ex vivo model used was effective for the assessment of these biomechanical parameters. In addition, microdamage was not influenced by the drilling rotation speed in this experimental condition.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372022000100325&tlng=enBone and bonesHistologyOsteotomy |
| spellingShingle | Isabella de Almeida FRANCISQUINI Gustavo Davi RABELO Neuza Maria Souza Picorelli ASSIS Bruno Salles SOTTO-MAIOR Pedro Henrique Justino Oliveira LIMIRIO Karina Lopes DEVITO Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocols RGO: Revista Gaúcha de Odontologia Bone and bones Histology Osteotomy |
| title | Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocols |
| title_full | Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocols |
| title_fullStr | Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocols |
| title_full_unstemmed | Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocols |
| title_short | Implant site preparation-related bone microdamage: An alternative ex vivo analysis of drilling speed protocols |
| title_sort | implant site preparation related bone microdamage an alternative ex vivo analysis of drilling speed protocols |
| topic | Bone and bones Histology Osteotomy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372022000100325&tlng=en |
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