Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica Rice
Starch branching enzyme IIb (BEIIb) and soluble starch synthase IIa (SSIIa) play important roles in starch biosynthesis in cereals. Deficiency in the BEIIb gene produces the <i>amylose extender</i> (<i>ae</i>) mutant rice strain with increased amylose content (AC) and changes...
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MDPI AG
2022-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/1/119 |
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| author | Yaqi Hu Yanni Zhang Shouwu Yu Guofu Deng Gaoxing Dai Jinsong Bao |
| author_facet | Yaqi Hu Yanni Zhang Shouwu Yu Guofu Deng Gaoxing Dai Jinsong Bao |
| author_sort | Yaqi Hu |
| collection | DOAJ |
| description | Starch branching enzyme IIb (BEIIb) and soluble starch synthase IIa (SSIIa) play important roles in starch biosynthesis in cereals. Deficiency in the BEIIb gene produces the <i>amylose extender</i> (<i>ae</i>) mutant rice strain with increased amylose content (AC) and changes in the amylopectin structure. The SSIIa gene is responsible for the genetic control of gelatinization temperature (GT). The combined effects of <i>BEIIb</i> and <i>SSIIa</i> alleles on the AC, fine structures, and physicochemical properties of starches from 12 rice accessions including 10 recombinant inbred lines (RIL) and their two parents were examined in this study. Under the active <i>BEIIb</i> background, starches with the <i>SSIIa</i>-GC allele showed a higher GT than those with the <i>SSIIa</i>-TT allele, resulting from a lower proportion of A chain and a larger proportion of B1 chains in the amylopectin of <i>SSIIa</i>-GC. However, starch with the <i>BEIIb</i> mutant allele (<i>be2b</i>) in combination with any <i>SSIIa</i> genotype displayed more amylose long chains, higher amylose content, B2 and B3 chains, and molecular order, but smaller relative crystallinity and proportion of amylopectin A and B1 chains than those with <i>BEIIb</i>, leading to a higher GT and lower paste viscosities. These results suggest that <i>BEIIb</i> is more important in determining the structural and physicochemical properties than <i>SSIIa</i>. These results provide additional insights into the structure-function relationship in indica rice rather than that in japonica rice and are useful for breeding rice with high amylose content and high resistant starch. |
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| last_indexed | 2024-03-11T10:00:41Z |
| publishDate | 2022-12-01 |
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| series | Foods |
| spelling | doaj.art-4236967ffa0c4852b168f48e64ed8def2023-11-16T15:22:39ZengMDPI AGFoods2304-81582022-12-0112111910.3390/foods12010119Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica RiceYaqi Hu0Yanni Zhang1Shouwu Yu2Guofu Deng3Gaoxing Dai4Jinsong Bao5Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaInstitute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaInstitute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaRice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaRice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaInstitute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaStarch branching enzyme IIb (BEIIb) and soluble starch synthase IIa (SSIIa) play important roles in starch biosynthesis in cereals. Deficiency in the BEIIb gene produces the <i>amylose extender</i> (<i>ae</i>) mutant rice strain with increased amylose content (AC) and changes in the amylopectin structure. The SSIIa gene is responsible for the genetic control of gelatinization temperature (GT). The combined effects of <i>BEIIb</i> and <i>SSIIa</i> alleles on the AC, fine structures, and physicochemical properties of starches from 12 rice accessions including 10 recombinant inbred lines (RIL) and their two parents were examined in this study. Under the active <i>BEIIb</i> background, starches with the <i>SSIIa</i>-GC allele showed a higher GT than those with the <i>SSIIa</i>-TT allele, resulting from a lower proportion of A chain and a larger proportion of B1 chains in the amylopectin of <i>SSIIa</i>-GC. However, starch with the <i>BEIIb</i> mutant allele (<i>be2b</i>) in combination with any <i>SSIIa</i> genotype displayed more amylose long chains, higher amylose content, B2 and B3 chains, and molecular order, but smaller relative crystallinity and proportion of amylopectin A and B1 chains than those with <i>BEIIb</i>, leading to a higher GT and lower paste viscosities. These results suggest that <i>BEIIb</i> is more important in determining the structural and physicochemical properties than <i>SSIIa</i>. These results provide additional insights into the structure-function relationship in indica rice rather than that in japonica rice and are useful for breeding rice with high amylose content and high resistant starch.https://www.mdpi.com/2304-8158/12/1/119ricestarch synthasebranching enzymestarch structurefunctional property |
| spellingShingle | Yaqi Hu Yanni Zhang Shouwu Yu Guofu Deng Gaoxing Dai Jinsong Bao Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica Rice Foods rice starch synthase branching enzyme starch structure functional property |
| title | Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica Rice |
| title_full | Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica Rice |
| title_fullStr | Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica Rice |
| title_full_unstemmed | Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica Rice |
| title_short | Combined Effects of <i>BEIIb</i> and <i>SSIIa</i> Alleles on Amylose Contents, Starch Fine Structures and Physicochemical Properties of Indica Rice |
| title_sort | combined effects of i beiib i and i ssiia i alleles on amylose contents starch fine structures and physicochemical properties of indica rice |
| topic | rice starch synthase branching enzyme starch structure functional property |
| url | https://www.mdpi.com/2304-8158/12/1/119 |
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