Ex vivo cytosolic delivery of functional macromolecules to immune cells.
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformat...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4395260?pdf=render |
| _version_ | 1818696194009858048 |
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| author | Armon Sharei Radiana Trifonova Siddharth Jhunjhunwala George C Hartoularos Alexandra T Eyerman Abigail Lytton-Jean Mathieu Angin Siddhartha Sharma Roberta Poceviciute Shirley Mao Megan Heimann Sophia Liu Tanya Talkar Omar F Khan Marylyn Addo Ulrich H von Andrian Daniel G Anderson Robert Langer Judy Lieberman Klavs F Jensen |
| author_facet | Armon Sharei Radiana Trifonova Siddharth Jhunjhunwala George C Hartoularos Alexandra T Eyerman Abigail Lytton-Jean Mathieu Angin Siddhartha Sharma Roberta Poceviciute Shirley Mao Megan Heimann Sophia Liu Tanya Talkar Omar F Khan Marylyn Addo Ulrich H von Andrian Daniel G Anderson Robert Langer Judy Lieberman Klavs F Jensen |
| author_sort | Armon Sharei |
| collection | DOAJ |
| description | Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies. |
| first_indexed | 2024-12-17T13:57:29Z |
| format | Article |
| id | doaj.art-4237992de75147fcb1684e435e7acb07 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T13:57:29Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4237992de75147fcb1684e435e7acb072022-12-21T21:45:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e011880310.1371/journal.pone.0118803Ex vivo cytosolic delivery of functional macromolecules to immune cells.Armon ShareiRadiana TrifonovaSiddharth JhunjhunwalaGeorge C HartoularosAlexandra T EyermanAbigail Lytton-JeanMathieu AnginSiddhartha SharmaRoberta PoceviciuteShirley MaoMegan HeimannSophia LiuTanya TalkarOmar F KhanMarylyn AddoUlrich H von AndrianDaniel G AndersonRobert LangerJudy LiebermanKlavs F JensenIntracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.http://europepmc.org/articles/PMC4395260?pdf=render |
| spellingShingle | Armon Sharei Radiana Trifonova Siddharth Jhunjhunwala George C Hartoularos Alexandra T Eyerman Abigail Lytton-Jean Mathieu Angin Siddhartha Sharma Roberta Poceviciute Shirley Mao Megan Heimann Sophia Liu Tanya Talkar Omar F Khan Marylyn Addo Ulrich H von Andrian Daniel G Anderson Robert Langer Judy Lieberman Klavs F Jensen Ex vivo cytosolic delivery of functional macromolecules to immune cells. PLoS ONE |
| title | Ex vivo cytosolic delivery of functional macromolecules to immune cells. |
| title_full | Ex vivo cytosolic delivery of functional macromolecules to immune cells. |
| title_fullStr | Ex vivo cytosolic delivery of functional macromolecules to immune cells. |
| title_full_unstemmed | Ex vivo cytosolic delivery of functional macromolecules to immune cells. |
| title_short | Ex vivo cytosolic delivery of functional macromolecules to immune cells. |
| title_sort | ex vivo cytosolic delivery of functional macromolecules to immune cells |
| url | http://europepmc.org/articles/PMC4395260?pdf=render |
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