Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.)
Abstract Background N 6-methyladenosine (m6A) is one of the common internal RNA modifications found in eukaryotes. The m6A modification can regulate various biological processes in organisms through the modulation of alternative splicing, alternative polyadenylation, folding, translation, localizati...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-02-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-04813-2 |
| _version_ | 1797275273030270976 |
|---|---|
| author | Huan Su Lijun Meng Zechao Qu Wei Zhang Nan Liu Peijian Cao Jingjing Jin |
| author_facet | Huan Su Lijun Meng Zechao Qu Wei Zhang Nan Liu Peijian Cao Jingjing Jin |
| author_sort | Huan Su |
| collection | DOAJ |
| description | Abstract Background N 6-methyladenosine (m6A) is one of the common internal RNA modifications found in eukaryotes. The m6A modification can regulate various biological processes in organisms through the modulation of alternative splicing, alternative polyadenylation, folding, translation, localization, transport, and decay of multiple types of RNA, without altering the nucleotide sequence. The three components involved in m6A modification, namely writer, eraser, and reader, mediate the abundance of RNA m6A modification through complex collaborative actions. Currently, research on m6A regulatory genes in plants is still in its infancy. Results In this study, we identified 52 candidate m6A regulatory genes in common tobacco (Nicotiana tabacum L.). Gene structure, conserved domains, and motif analysis showed structural and functional diversity among different subgroups of tobacco m6A regulatory genes. The amplification of m6A regulatory genes were mainly driven by polyploidization and dispersed duplication, and duplicated genes evolved through purified selection. Based on the potential regulatory network and expression pattern analysis of m6A regulatory genes, a significant number of m6A regulatory genes might play important roles in growth, development, and stress response processes. Furthermore, we have confirmed the critical role of NtFIP37B, an m6A writer gene in tobacco, in enhancing drought resistance. Conclusions This study provides useful information for better understanding the evolution of m6A regulatory genes and the role of m6A modification in tobacco stress response, and lays the foundation for further elucidating the function of m6A regulatory genes in tobacco. |
| first_indexed | 2024-03-07T15:10:55Z |
| format | Article |
| id | doaj.art-423984d1bdae4c94b3789a0ca2fe9c6e |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:10:55Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-423984d1bdae4c94b3789a0ca2fe9c6e2024-03-05T18:38:06ZengBMCBMC Plant Biology1471-22292024-02-0124111810.1186/s12870-024-04813-2Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.)Huan Su0Lijun Meng1Zechao Qu2Wei Zhang3Nan Liu4Peijian Cao5Jingjing Jin6Beijing Life Science AcademyChina Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTCChina Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTCChina National Tobacco Quality Supervision & Test CenterChina National Tobacco Quality Supervision & Test CenterBeijing Life Science AcademyBeijing Life Science AcademyAbstract Background N 6-methyladenosine (m6A) is one of the common internal RNA modifications found in eukaryotes. The m6A modification can regulate various biological processes in organisms through the modulation of alternative splicing, alternative polyadenylation, folding, translation, localization, transport, and decay of multiple types of RNA, without altering the nucleotide sequence. The three components involved in m6A modification, namely writer, eraser, and reader, mediate the abundance of RNA m6A modification through complex collaborative actions. Currently, research on m6A regulatory genes in plants is still in its infancy. Results In this study, we identified 52 candidate m6A regulatory genes in common tobacco (Nicotiana tabacum L.). Gene structure, conserved domains, and motif analysis showed structural and functional diversity among different subgroups of tobacco m6A regulatory genes. The amplification of m6A regulatory genes were mainly driven by polyploidization and dispersed duplication, and duplicated genes evolved through purified selection. Based on the potential regulatory network and expression pattern analysis of m6A regulatory genes, a significant number of m6A regulatory genes might play important roles in growth, development, and stress response processes. Furthermore, we have confirmed the critical role of NtFIP37B, an m6A writer gene in tobacco, in enhancing drought resistance. Conclusions This study provides useful information for better understanding the evolution of m6A regulatory genes and the role of m6A modification in tobacco stress response, and lays the foundation for further elucidating the function of m6A regulatory genes in tobacco.https://doi.org/10.1186/s12870-024-04813-2RNA methylationm6A regulatorsTobaccoExpression patternDrought stress |
| spellingShingle | Huan Su Lijun Meng Zechao Qu Wei Zhang Nan Liu Peijian Cao Jingjing Jin Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.) BMC Plant Biology RNA methylation m6A regulators Tobacco Expression pattern Drought stress |
| title | Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.) |
| title_full | Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.) |
| title_fullStr | Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.) |
| title_full_unstemmed | Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.) |
| title_short | Genome-wide identification of the N 6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.) |
| title_sort | genome wide identification of the n 6 methyladenosine regulatory genes reveals ntfip37b increases drought resistance of tobacco nicotiana tabacum l |
| topic | RNA methylation m6A regulators Tobacco Expression pattern Drought stress |
| url | https://doi.org/10.1186/s12870-024-04813-2 |
| work_keys_str_mv | AT huansu genomewideidentificationofthen6methyladenosineregulatorygenesrevealsntfip37bincreasesdroughtresistanceoftobacconicotianatabacuml AT lijunmeng genomewideidentificationofthen6methyladenosineregulatorygenesrevealsntfip37bincreasesdroughtresistanceoftobacconicotianatabacuml AT zechaoqu genomewideidentificationofthen6methyladenosineregulatorygenesrevealsntfip37bincreasesdroughtresistanceoftobacconicotianatabacuml AT weizhang genomewideidentificationofthen6methyladenosineregulatorygenesrevealsntfip37bincreasesdroughtresistanceoftobacconicotianatabacuml AT nanliu genomewideidentificationofthen6methyladenosineregulatorygenesrevealsntfip37bincreasesdroughtresistanceoftobacconicotianatabacuml AT peijiancao genomewideidentificationofthen6methyladenosineregulatorygenesrevealsntfip37bincreasesdroughtresistanceoftobacconicotianatabacuml AT jingjingjin genomewideidentificationofthen6methyladenosineregulatorygenesrevealsntfip37bincreasesdroughtresistanceoftobacconicotianatabacuml |