Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs.
BACKGROUND:Previous studies have shown that plant mitochondrial movements are myosin-based along actin filaments, which undergo continuous turnover by the exchange of actin subunits from existing filaments. Although earlier studies revealed that actin filament dynamics are essential for many functio...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-06-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2694364?pdf=render |
| _version_ | 1819069377487568896 |
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| author | Maozhong Zheng Martina Beck Jens Müller Tong Chen Xiaohua Wang Feng Wang Qinli Wang Yuqing Wang Frantisek Baluska David C Logan Jozef Samaj Jinxing Lin |
| author_facet | Maozhong Zheng Martina Beck Jens Müller Tong Chen Xiaohua Wang Feng Wang Qinli Wang Yuqing Wang Frantisek Baluska David C Logan Jozef Samaj Jinxing Lin |
| author_sort | Maozhong Zheng |
| collection | DOAJ |
| description | BACKGROUND:Previous studies have shown that plant mitochondrial movements are myosin-based along actin filaments, which undergo continuous turnover by the exchange of actin subunits from existing filaments. Although earlier studies revealed that actin filament dynamics are essential for many functions of the actin cytoskeleton, there are little data connecting actin dynamics and mitochondrial movements. METHODOLOGY/PRINCIPAL FINDINGS:We addressed the role of actin filament dynamics in the control of mitochondrial movements by treating cells with various pharmaceuticals that affect actin filament assembly and disassembly. Confocal microscopy of Arabidopsis thaliana root hairs expressing GFP-FABD2 as an actin filament reporter showed that mitochondrial distribution was in agreement with the arrangement of actin filaments in root hairs at different developmental stages. Analyses of mitochondrial trajectories and instantaneous velocities immediately following pharmacological perturbation of the cytoskeleton using variable-angle evanescent wave microscopy and/or spinning disk confocal microscopy revealed that mitochondrial velocities were regulated by myosin activity and actin filament dynamics. Furthermore, simultaneous visualization of mitochondria and actin filaments suggested that mitochondrial positioning might involve depolymerization of actin filaments on the surface of mitochondria. CONCLUSIONS/SIGNIFICANCE:Base on these results we propose a mechanism for the regulation of mitochondrial speed of movements, positioning, and direction of movements that combines the coordinated activity of myosin and the rate of actin turnover, together with microtubule dynamics, which directs the positioning of actin polymerization events. |
| first_indexed | 2024-12-21T16:49:05Z |
| format | Article |
| id | doaj.art-423bf1c6e61a4bb389d93fab7dcc20e6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T16:49:05Z |
| publishDate | 2009-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-423bf1c6e61a4bb389d93fab7dcc20e62022-12-21T18:56:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-06-0146e596110.1371/journal.pone.0005961Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs.Maozhong ZhengMartina BeckJens MüllerTong ChenXiaohua WangFeng WangQinli WangYuqing WangFrantisek BaluskaDavid C LoganJozef SamajJinxing LinBACKGROUND:Previous studies have shown that plant mitochondrial movements are myosin-based along actin filaments, which undergo continuous turnover by the exchange of actin subunits from existing filaments. Although earlier studies revealed that actin filament dynamics are essential for many functions of the actin cytoskeleton, there are little data connecting actin dynamics and mitochondrial movements. METHODOLOGY/PRINCIPAL FINDINGS:We addressed the role of actin filament dynamics in the control of mitochondrial movements by treating cells with various pharmaceuticals that affect actin filament assembly and disassembly. Confocal microscopy of Arabidopsis thaliana root hairs expressing GFP-FABD2 as an actin filament reporter showed that mitochondrial distribution was in agreement with the arrangement of actin filaments in root hairs at different developmental stages. Analyses of mitochondrial trajectories and instantaneous velocities immediately following pharmacological perturbation of the cytoskeleton using variable-angle evanescent wave microscopy and/or spinning disk confocal microscopy revealed that mitochondrial velocities were regulated by myosin activity and actin filament dynamics. Furthermore, simultaneous visualization of mitochondria and actin filaments suggested that mitochondrial positioning might involve depolymerization of actin filaments on the surface of mitochondria. CONCLUSIONS/SIGNIFICANCE:Base on these results we propose a mechanism for the regulation of mitochondrial speed of movements, positioning, and direction of movements that combines the coordinated activity of myosin and the rate of actin turnover, together with microtubule dynamics, which directs the positioning of actin polymerization events.http://europepmc.org/articles/PMC2694364?pdf=render |
| spellingShingle | Maozhong Zheng Martina Beck Jens Müller Tong Chen Xiaohua Wang Feng Wang Qinli Wang Yuqing Wang Frantisek Baluska David C Logan Jozef Samaj Jinxing Lin Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs. PLoS ONE |
| title | Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs. |
| title_full | Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs. |
| title_fullStr | Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs. |
| title_full_unstemmed | Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs. |
| title_short | Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs. |
| title_sort | actin turnover is required for myosin dependent mitochondrial movements in arabidopsis root hairs |
| url | http://europepmc.org/articles/PMC2694364?pdf=render |
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