CRISPR-Cas9 Mediated Capsule Gene Silencing in Escherichia coli
Background: Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli. Materials and Methods: We suppressed genes involved in capsule expression of E.coli by CRIS...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Shahid Beheshti University of Medical Sciences
2018-10-01
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| Series: | Novelty in Biomedicine |
| Subjects: | |
| Online Access: | http://journals.sbmu.ac.ir/nbm/article/view/21672 |
| Summary: | Background: Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli.
Materials and Methods: We suppressed genes involved in capsule expression of E.coli by CRISPR cas9 process. The constructed E.coli was confirmed by microscopic smear, transmission electron microscopy and T7 phage influence assay.
Results: The results were shown that the inhibition of capsule production was carried out successfully and there was not any capsule layer around the bacteria.
Conclusion: E. coli without any capsule around may proper for replacement of it with other molecules in future. |
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| ISSN: | 2345-3346 2345-3907 |