Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, r...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1359498 |
| _version_ | 1818982648164384768 |
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| author | Jianyi Wang Qi Wang Yongjun Zheng Tao Peng Kai Yao Sanlei Xie Xiya Zhang Xi Xia Jiancheng Li Haiyang Jiang |
| author_facet | Jianyi Wang Qi Wang Yongjun Zheng Tao Peng Kai Yao Sanlei Xie Xiya Zhang Xi Xia Jiancheng Li Haiyang Jiang |
| author_sort | Jianyi Wang |
| collection | DOAJ |
| description | A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC–MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis. |
| first_indexed | 2024-12-20T17:50:33Z |
| format | Article |
| id | doaj.art-4249f6507c67419b83ab3962f8ae4e4f |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-20T17:50:33Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-4249f6507c67419b83ab3962f8ae4e4f2022-12-21T19:30:51ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-01291566610.1080/09540105.2017.13594981359498Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milkJianyi Wang0Qi Wang1Yongjun Zheng2Tao Peng3Kai Yao4Sanlei Xie5Xiya Zhang6Xi Xia7Jiancheng Li8Haiyang Jiang9Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityDepartment of Mechanical and Electrical Engineering, College of Engineering, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural UniversityA competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC–MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis.http://dx.doi.org/10.1080/09540105.2017.1359498lateral flow immunoassayquantitative detectionfluorescent microspherechloramphenicolthiamphenicolflorfenicolmilk |
| spellingShingle | Jianyi Wang Qi Wang Yongjun Zheng Tao Peng Kai Yao Sanlei Xie Xiya Zhang Xi Xia Jiancheng Li Haiyang Jiang Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk Food and Agricultural Immunology lateral flow immunoassay quantitative detection fluorescent microsphere chloramphenicol thiamphenicol florfenicol milk |
| title | Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk |
| title_full | Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk |
| title_fullStr | Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk |
| title_full_unstemmed | Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk |
| title_short | Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk |
| title_sort | development of a quantitative fluorescence based lateral flow immunoassay for determination of chloramphenicol thiamphenicol and florfenicol in milk |
| topic | lateral flow immunoassay quantitative detection fluorescent microsphere chloramphenicol thiamphenicol florfenicol milk |
| url | http://dx.doi.org/10.1080/09540105.2017.1359498 |
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