Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations
Abstract Background Both mild and conventional controlled ovarian stimulation are the frequently used protocols for poor ovarian responders. However, there are some debates about which treatment is better. Moreover, little is known about the follicular physiology after the two ovarian stimulation pr...
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | Reproductive Biology and Endocrinology |
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| Online Access: | https://doi.org/10.1186/s12958-022-00926-1 |
| _version_ | 1811335489468235776 |
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| author | Xiaoping Liu Huisi Mai Panyu Chen Zhiqiang Zhang Taibao Wu Jianhui Chen Peng Sun Chuanchuan Zhou Xiaoyan Liang Rui Huang |
| author_facet | Xiaoping Liu Huisi Mai Panyu Chen Zhiqiang Zhang Taibao Wu Jianhui Chen Peng Sun Chuanchuan Zhou Xiaoyan Liang Rui Huang |
| author_sort | Xiaoping Liu |
| collection | DOAJ |
| description | Abstract Background Both mild and conventional controlled ovarian stimulation are the frequently used protocols for poor ovarian responders. However, there are some debates about which treatment is better. Moreover, little is known about the follicular physiology after the two ovarian stimulation protocols. This study was intended to investigate the features in granulosa cells and follicular fluid micro-environment after the two different ovarian stimulation protocols in poor responders. Methods Granulosa cells RNA were sequenced using Illumina Hiseq technology. Specific differently expressed genes and proteins were verified by real-time quantitative PCR and Western blot analysis. Moreover, hormone and cytokine concentrations in the follicular fluid were measured by electrochemiluminescence immunoassay and enzyme-linked immunoabsorbent assay. The correlation between the results of molecular experiments and the laboratory outcomes were analyzed by Spearman correlation analysis. Results The differentially expressed genes between the two groups were involved in 4 signaling pathways related to the follicular development; three proteins pertinent to the TGF-β signaling pathway were expressed differently in granulosa cells between the two, and the constituents in the follicular fluid were also different. Further, a correlation between the TGF-β signaling pathway and the good-quality embryo was observed. Conclusions The present study made a comparison for the first time in the transcriptome of human granulosa cells and the follicular fluid micro-environment between poor responders with the conventional controlled ovarian stimulation or the mild ovarian stimulation, showing that the TGF-β signaling pathway may correlate with the good-quality of embryos in the mild group, which may be instrumental to the choice of optimal management for IVF patients. |
| first_indexed | 2024-04-13T17:25:48Z |
| format | Article |
| id | doaj.art-424db17ec7ad41b7a88db4591cd8928e |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-13T17:25:48Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-424db17ec7ad41b7a88db4591cd8928e2022-12-22T02:37:48ZengBMCReproductive Biology and Endocrinology1477-78272022-03-0120111310.1186/s12958-022-00926-1Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulationsXiaoping Liu0Huisi Mai1Panyu Chen2Zhiqiang Zhang3Taibao Wu4Jianhui Chen5Peng Sun6Chuanchuan Zhou7Xiaoyan Liang8Rui Huang9Reproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityReproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen UniversityAbstract Background Both mild and conventional controlled ovarian stimulation are the frequently used protocols for poor ovarian responders. However, there are some debates about which treatment is better. Moreover, little is known about the follicular physiology after the two ovarian stimulation protocols. This study was intended to investigate the features in granulosa cells and follicular fluid micro-environment after the two different ovarian stimulation protocols in poor responders. Methods Granulosa cells RNA were sequenced using Illumina Hiseq technology. Specific differently expressed genes and proteins were verified by real-time quantitative PCR and Western blot analysis. Moreover, hormone and cytokine concentrations in the follicular fluid were measured by electrochemiluminescence immunoassay and enzyme-linked immunoabsorbent assay. The correlation between the results of molecular experiments and the laboratory outcomes were analyzed by Spearman correlation analysis. Results The differentially expressed genes between the two groups were involved in 4 signaling pathways related to the follicular development; three proteins pertinent to the TGF-β signaling pathway were expressed differently in granulosa cells between the two, and the constituents in the follicular fluid were also different. Further, a correlation between the TGF-β signaling pathway and the good-quality embryo was observed. Conclusions The present study made a comparison for the first time in the transcriptome of human granulosa cells and the follicular fluid micro-environment between poor responders with the conventional controlled ovarian stimulation or the mild ovarian stimulation, showing that the TGF-β signaling pathway may correlate with the good-quality of embryos in the mild group, which may be instrumental to the choice of optimal management for IVF patients.https://doi.org/10.1186/s12958-022-00926-1Transcriptome analysisFollicular fluid micro-environmentMild ovarian stimulationControlled ovarian stimulationPoor ovarian responseGranulosa cells |
| spellingShingle | Xiaoping Liu Huisi Mai Panyu Chen Zhiqiang Zhang Taibao Wu Jianhui Chen Peng Sun Chuanchuan Zhou Xiaoyan Liang Rui Huang Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations Reproductive Biology and Endocrinology Transcriptome analysis Follicular fluid micro-environment Mild ovarian stimulation Controlled ovarian stimulation Poor ovarian response Granulosa cells |
| title | Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations |
| title_full | Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations |
| title_fullStr | Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations |
| title_full_unstemmed | Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations |
| title_short | Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations |
| title_sort | comparative analyses in transcriptome of human granulosa cells and follicular fluid micro environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations |
| topic | Transcriptome analysis Follicular fluid micro-environment Mild ovarian stimulation Controlled ovarian stimulation Poor ovarian response Granulosa cells |
| url | https://doi.org/10.1186/s12958-022-00926-1 |
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