Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice
Obesity-induced adipose tissue dysfunction and disorders of glycolipid metabolism have become a worldwide research priority. Zfp217 plays a crucial role in adipogenesis of 3T3-L1 preadipocytes, but about its functions in animal models are not yet clear. To explore the role of Zfp217 in high-fat diet...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/10/5390 |
| _version_ | 1797533335723966464 |
|---|---|
| author | Qianhui Zeng Nannan Wang Yaru Zhang Yuxuan Yang Shuangshuang Li Rong Zheng Jin Chai Tong Qiao Siwen Jiang |
| author_facet | Qianhui Zeng Nannan Wang Yaru Zhang Yuxuan Yang Shuangshuang Li Rong Zheng Jin Chai Tong Qiao Siwen Jiang |
| author_sort | Qianhui Zeng |
| collection | DOAJ |
| description | Obesity-induced adipose tissue dysfunction and disorders of glycolipid metabolism have become a worldwide research priority. Zfp217 plays a crucial role in adipogenesis of 3T3-L1 preadipocytes, but about its functions in animal models are not yet clear. To explore the role of Zfp217 in high-fat diet (HFD)-induced obese mice, global Zfp217 heterozygous knockout (Zfp217<sup>+/−</sup>) mice were constructed. Zfp217<sup>+/−</sup> mice and Zfp217<sup>+/+</sup> mice fed a normal chow diet (NC) did not differ significantly in weight gain, percent body fat mass, glucose tolerance, or insulin sensitivity. When challenged with HFD, Zfp217<sup>+/−</sup> mice had less weight gain than Zfp217<sup>+/+</sup> mice. Histological observations revealed that Zfp217<sup>+/−</sup> mice fed a high-fat diet had much smaller white adipocytes in inguinal white adipose tissue (iWAT). Zfp217<sup>+/−</sup> mice had improved metabolic profiles, including improved glucose tolerance, enhanced insulin sensitivity, and increased energy expenditure compared to the Zfp217<sup>+/+</sup> mice under HFD. We found that adipogenesis-related genes were increased and metabolic thermogenesis-related genes were decreased in the iWAT of HFD-fed Zfp217<sup>+/+</sup> mice compared to Zfp217<sup>+/−</sup> mice. In addition, adipogenesis was markedly reduced in mouse embryonic fibroblasts (MEFs) from Zfp217-deleted mice. Together, these data indicate that Zfp217 is a regulator of energy metabolism and it is likely to provide novel insight into treatment for obesity. |
| first_indexed | 2024-03-10T11:13:06Z |
| format | Article |
| id | doaj.art-425045ac657e49698043c7ff64e76756 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:13:06Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-425045ac657e49698043c7ff64e767562023-11-21T20:37:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012210539010.3390/ijms22105390Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in MiceQianhui Zeng0Nannan Wang1Yaru Zhang2Yuxuan Yang3Shuangshuang Li4Rong Zheng5Jin Chai6Tong Qiao7Siwen Jiang8Key Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaObesity-induced adipose tissue dysfunction and disorders of glycolipid metabolism have become a worldwide research priority. Zfp217 plays a crucial role in adipogenesis of 3T3-L1 preadipocytes, but about its functions in animal models are not yet clear. To explore the role of Zfp217 in high-fat diet (HFD)-induced obese mice, global Zfp217 heterozygous knockout (Zfp217<sup>+/−</sup>) mice were constructed. Zfp217<sup>+/−</sup> mice and Zfp217<sup>+/+</sup> mice fed a normal chow diet (NC) did not differ significantly in weight gain, percent body fat mass, glucose tolerance, or insulin sensitivity. When challenged with HFD, Zfp217<sup>+/−</sup> mice had less weight gain than Zfp217<sup>+/+</sup> mice. Histological observations revealed that Zfp217<sup>+/−</sup> mice fed a high-fat diet had much smaller white adipocytes in inguinal white adipose tissue (iWAT). Zfp217<sup>+/−</sup> mice had improved metabolic profiles, including improved glucose tolerance, enhanced insulin sensitivity, and increased energy expenditure compared to the Zfp217<sup>+/+</sup> mice under HFD. We found that adipogenesis-related genes were increased and metabolic thermogenesis-related genes were decreased in the iWAT of HFD-fed Zfp217<sup>+/+</sup> mice compared to Zfp217<sup>+/−</sup> mice. In addition, adipogenesis was markedly reduced in mouse embryonic fibroblasts (MEFs) from Zfp217-deleted mice. Together, these data indicate that Zfp217 is a regulator of energy metabolism and it is likely to provide novel insight into treatment for obesity.https://www.mdpi.com/1422-0067/22/10/5390Zfp217obesityadipogenesisglucose toleranceinsulin sensitivityenergy expenditure |
| spellingShingle | Qianhui Zeng Nannan Wang Yaru Zhang Yuxuan Yang Shuangshuang Li Rong Zheng Jin Chai Tong Qiao Siwen Jiang Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice International Journal of Molecular Sciences Zfp217 obesity adipogenesis glucose tolerance insulin sensitivity energy expenditure |
| title | Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice |
| title_full | Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice |
| title_fullStr | Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice |
| title_full_unstemmed | Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice |
| title_short | Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice |
| title_sort | partial deficiency of zfp217 resists high fat diet induced obesity by increasing energy metabolism in mice |
| topic | Zfp217 obesity adipogenesis glucose tolerance insulin sensitivity energy expenditure |
| url | https://www.mdpi.com/1422-0067/22/10/5390 |
| work_keys_str_mv | AT qianhuizeng partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT nannanwang partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT yaruzhang partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT yuxuanyang partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT shuangshuangli partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT rongzheng partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT jinchai partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT tongqiao partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice AT siwenjiang partialdeficiencyofzfp217resistshighfatdietinducedobesitybyincreasingenergymetabolisminmice |