Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression
Introduction: Monosodium glutamate (MSG) is one of the commonest food additives in modern nutrition. Recent studies showed the modulatory effect of MSG on rat thymocyte apoptosis in vivo and under in vitro conditions. However, the influence of MSG on thymocyte proliferation is largely unknown. Mater...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2006-12-01
|
| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | http://www.termedia.pl/magazine.php?magazine_id=19&article_id=7335&magazine_subpage=FULL_TEXT |
| _version_ | 1818335280364519424 |
|---|---|
| author | Voja Pavlović Snežana Cekić |
| author_facet | Voja Pavlović Snežana Cekić |
| author_sort | Voja Pavlović |
| collection | DOAJ |
| description | Introduction: Monosodium glutamate (MSG) is one of the commonest food additives in modern nutrition. Recent studies showed the modulatory effect of MSG on rat thymocyte apoptosis in vivo and under in vitro conditions. However, the influence of MSG on thymocyte proliferation is largely unknown. Material and methods: We evaluated the effect of increased MSG concentrations on thymocyte proliferation, as well as the expression of their two apoptosis-related proteins, Bcl-2 and Bax. Proliferation was detected by PCNA monoclonal antibody, and cells were analyzed using a flow cytometer. Expression of Bcl-2 and Bax protein were determined with flow cytometry, using respective monoclonal antibodies. Results: The results obtained in our study demonstrate that MSG significantly decreased thymocyte proliferation, in a dose dependent manner, followed by significantly increased cytotoxicity. The expression of Bcl-2 protein was significantly decreased, while Bax protein expression was not changed in our study. Conclusions: The results presented in our study indicate that MSG significantly modulated intensity of thymocyte proliferation as a consequence of increased cytotoxicity. The expression of Bcl-2 and Bax proteins suggests that this protein level is an important event in thymocyte cytotoxicity, triggered by MSG |
| first_indexed | 2024-12-13T14:20:55Z |
| format | Article |
| id | doaj.art-4252fd2ef3ce4087b7175823c8f97018 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 |
| language | English |
| last_indexed | 2024-12-13T14:20:55Z |
| publishDate | 2006-12-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-4252fd2ef3ce4087b7175823c8f970182022-12-21T23:42:06ZengTermedia Publishing HouseArchives of Medical Science1734-19222006-12-0124247251Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expressionVoja PavlovićSnežana CekićIntroduction: Monosodium glutamate (MSG) is one of the commonest food additives in modern nutrition. Recent studies showed the modulatory effect of MSG on rat thymocyte apoptosis in vivo and under in vitro conditions. However, the influence of MSG on thymocyte proliferation is largely unknown. Material and methods: We evaluated the effect of increased MSG concentrations on thymocyte proliferation, as well as the expression of their two apoptosis-related proteins, Bcl-2 and Bax. Proliferation was detected by PCNA monoclonal antibody, and cells were analyzed using a flow cytometer. Expression of Bcl-2 and Bax protein were determined with flow cytometry, using respective monoclonal antibodies. Results: The results obtained in our study demonstrate that MSG significantly decreased thymocyte proliferation, in a dose dependent manner, followed by significantly increased cytotoxicity. The expression of Bcl-2 protein was significantly decreased, while Bax protein expression was not changed in our study. Conclusions: The results presented in our study indicate that MSG significantly modulated intensity of thymocyte proliferation as a consequence of increased cytotoxicity. The expression of Bcl-2 and Bax proteins suggests that this protein level is an important event in thymocyte cytotoxicity, triggered by MSGhttp://www.termedia.pl/magazine.php?magazine_id=19&article_id=7335&magazine_subpage=FULL_TEXTmonosodium glutamateproliferationthymocytesBcl-2Bax |
| spellingShingle | Voja Pavlović Snežana Cekić Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression Archives of Medical Science monosodium glutamate proliferation thymocytes Bcl-2 Bax |
| title | Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression |
| title_full | Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression |
| title_fullStr | Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression |
| title_full_unstemmed | Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression |
| title_short | Original paper<br>The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression |
| title_sort | original paper br the effect of monosodium glutamate on rat thymocyte proliferation and bcl 2 bax protein expression |
| topic | monosodium glutamate proliferation thymocytes Bcl-2 Bax |
| url | http://www.termedia.pl/magazine.php?magazine_id=19&article_id=7335&magazine_subpage=FULL_TEXT |
| work_keys_str_mv | AT vojapavlovic originalpaperbrtheeffectofmonosodiumglutamateonratthymocyteproliferationandbcl2baxproteinexpression AT snezanacekic originalpaperbrtheeffectofmonosodiumglutamateonratthymocyteproliferationandbcl2baxproteinexpression |