Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i>
<i>Hemerocallis</i> is a characteristic vegetable with outstanding edible and medicinal value. Flavonoids are important bioactive components of <i>Hemerocallis</i>. To improve the extraction efficiency and detection accuracy of flavonoids from <i>Hemerocallis</i>,...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/9/11/1233 |
| _version_ | 1797459114986569728 |
|---|---|
| author | Jie Su Mengyue Jing Lijuan Zhang Chenzhi Liu Niping Xue Wenjing Yang Wei Zhang Feifan Hou Xiaomin Zhang Yanfang Wang Guoming Xing Sen Li |
| author_facet | Jie Su Mengyue Jing Lijuan Zhang Chenzhi Liu Niping Xue Wenjing Yang Wei Zhang Feifan Hou Xiaomin Zhang Yanfang Wang Guoming Xing Sen Li |
| author_sort | Jie Su |
| collection | DOAJ |
| description | <i>Hemerocallis</i> is a characteristic vegetable with outstanding edible and medicinal value. Flavonoids are important bioactive components of <i>Hemerocallis</i>. To improve the extraction efficiency and detection accuracy of flavonoids from <i>Hemerocallis</i>, we established a high-performance liquid chromatography (HPLC) detection system, which can simultaneously detect multiple flavonoids. In addition to the previously developed organic solvent extraction method, an ultrasonic-assisted extraction technique that uses fewer samples was established to extract flavonoids from <i>Hemerocallis</i>. The extraction conditions of the ultrasonic-assisted extraction were optimized via a single-factor experiment and a response surface experiment. The HPLC system detected and determined the contents of rutin, isoquercetin, myricetin, quercetin, apigenin, and diosmetin from 70 <i>Hemerocallis</i> germplasm resources. In addition, we evaluated the antioxidant activity of flavonoids in <i>Hemerocallis</i> using DPPH free radical scavenging capacity with ascorbic acid (Vc) as a positive control. The results showed that the optimum conditions for the ultrasonic extraction process were as follows: sample weight of 0.25 g, ethanol volume fraction of 72%, ethanol volume of 2.5 mL, and ultrasonic extraction time of 17 min. Under these conditions, flavonoid extraction had a strong scavenging effect on DPPH. With the increase in the sample solutions’ concentrations, its antioxidant capacity was gradually enhanced, and the DPPH scavenging rate reached 70.2%. The optimized ultrasonic-assisted extraction technology can increase the total content of six flavonoids in day lily bud by 59.01%, especially the content of rutin (increased by 64.41%) in <i>Hemerocallis</i> flower buds. Among 70 <i>Hemerocallis</i> plant resources, we selected materials H0087 and H0059 with high and stable flavonoid content, with the total content of six substances being 4390.54 ug/g and 3777.13 ug/g. Thus, this study provides a reference for extracting and determining flavonoid contents in <i>Hemerocallis</i> materials. It also provides a theoretical basis for high-quality individual plant breeding. |
| first_indexed | 2024-03-09T16:46:48Z |
| format | Article |
| id | doaj.art-4255257779164f9f840a7f5286cfe2fa |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T16:46:48Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-4255257779164f9f840a7f5286cfe2fa2023-11-24T14:45:21ZengMDPI AGHorticulturae2311-75242023-11-01911123310.3390/horticulturae9111233Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i>Jie Su0Mengyue Jing1Lijuan Zhang2Chenzhi Liu3Niping Xue4Wenjing Yang5Wei Zhang6Feifan Hou7Xiaomin Zhang8Yanfang Wang9Guoming Xing10Sen Li11College of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaGuangling Country Reform and Economy Affairs Center, Guangling 037500, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, China<i>Hemerocallis</i> is a characteristic vegetable with outstanding edible and medicinal value. Flavonoids are important bioactive components of <i>Hemerocallis</i>. To improve the extraction efficiency and detection accuracy of flavonoids from <i>Hemerocallis</i>, we established a high-performance liquid chromatography (HPLC) detection system, which can simultaneously detect multiple flavonoids. In addition to the previously developed organic solvent extraction method, an ultrasonic-assisted extraction technique that uses fewer samples was established to extract flavonoids from <i>Hemerocallis</i>. The extraction conditions of the ultrasonic-assisted extraction were optimized via a single-factor experiment and a response surface experiment. The HPLC system detected and determined the contents of rutin, isoquercetin, myricetin, quercetin, apigenin, and diosmetin from 70 <i>Hemerocallis</i> germplasm resources. In addition, we evaluated the antioxidant activity of flavonoids in <i>Hemerocallis</i> using DPPH free radical scavenging capacity with ascorbic acid (Vc) as a positive control. The results showed that the optimum conditions for the ultrasonic extraction process were as follows: sample weight of 0.25 g, ethanol volume fraction of 72%, ethanol volume of 2.5 mL, and ultrasonic extraction time of 17 min. Under these conditions, flavonoid extraction had a strong scavenging effect on DPPH. With the increase in the sample solutions’ concentrations, its antioxidant capacity was gradually enhanced, and the DPPH scavenging rate reached 70.2%. The optimized ultrasonic-assisted extraction technology can increase the total content of six flavonoids in day lily bud by 59.01%, especially the content of rutin (increased by 64.41%) in <i>Hemerocallis</i> flower buds. Among 70 <i>Hemerocallis</i> plant resources, we selected materials H0087 and H0059 with high and stable flavonoid content, with the total content of six substances being 4390.54 ug/g and 3777.13 ug/g. Thus, this study provides a reference for extracting and determining flavonoid contents in <i>Hemerocallis</i> materials. It also provides a theoretical basis for high-quality individual plant breeding.https://www.mdpi.com/2311-7524/9/11/1233<i>Hemerocallis</i>flavonoidsultrasonic extractionHPLC |
| spellingShingle | Jie Su Mengyue Jing Lijuan Zhang Chenzhi Liu Niping Xue Wenjing Yang Wei Zhang Feifan Hou Xiaomin Zhang Yanfang Wang Guoming Xing Sen Li Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i> Horticulturae <i>Hemerocallis</i> flavonoids ultrasonic extraction HPLC |
| title | Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i> |
| title_full | Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i> |
| title_fullStr | Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i> |
| title_full_unstemmed | Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i> |
| title_short | Establishment and Optimization of Flavonoid Extraction and Detection System for <i>Hemerocallis</i> |
| title_sort | establishment and optimization of flavonoid extraction and detection system for i hemerocallis i |
| topic | <i>Hemerocallis</i> flavonoids ultrasonic extraction HPLC |
| url | https://www.mdpi.com/2311-7524/9/11/1233 |
| work_keys_str_mv | AT jiesu establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT mengyuejing establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT lijuanzhang establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT chenzhiliu establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT nipingxue establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT wenjingyang establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT weizhang establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT feifanhou establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT xiaominzhang establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT yanfangwang establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT guomingxing establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi AT senli establishmentandoptimizationofflavonoidextractionanddetectionsystemforihemerocallisi |