Global analysis of protein lysine succinylation profiles in common wheat
Abstract Background Protein lysine succinylation is an important post-translational modification and plays a critical regulatory role in almost every aspects of cell metabolism in both eukaryotes and prokaryotes. Common wheat is one of the major global cereal crops. However, to date, little is known...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-017-3698-2 |
| _version_ | 1819291174919208960 |
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| author | Yumei Zhang Guangyuan Wang Limin Song Ping Mu Shu Wang Wenxing Liang Qi Lin |
| author_facet | Yumei Zhang Guangyuan Wang Limin Song Ping Mu Shu Wang Wenxing Liang Qi Lin |
| author_sort | Yumei Zhang |
| collection | DOAJ |
| description | Abstract Background Protein lysine succinylation is an important post-translational modification and plays a critical regulatory role in almost every aspects of cell metabolism in both eukaryotes and prokaryotes. Common wheat is one of the major global cereal crops. However, to date, little is known about the functions of lysine succinylation in this plant. Here, we performed a global analysis of lysine succinylation in wheat and examined its overlap with lysine acetylation. Results In total, 330 lysine succinylated modification sites were identified in 173 proteins. Bioinformatics analysis showed that the modified proteins are distributed in multiple subcellular compartments and are involved in a wide variety of biological processes such as photosynthesis and the Calvin-Benson cycle, suggesting an important role for lysine succinylation in these processes. Five putative succinylation motifs were identified. A protein interaction network analysis revealed that diverse interactions are modulated by protein succinylation. Moreover, 21 succinyl-lysine sites were found to be acetylated at the same position, and 33 proteins were modified by both acetylation and succinylation, suggesting an extensive overlap between succinylation and acetylation in common wheat. Comparative analysis indicated that lysine succinylation is conserved between common wheat and Brachypodium distachyon. Conclusions These results suggest that lysine succinylation is involved in diverse biological processes, especially in photosynthesis and carbon fixation. This systematic analysis represents the first global analysis of lysine succinylation in common wheat and provides an important resource for exploring the physiological role of lysine succinylation in this cereal crop and likely in all plants. |
| first_indexed | 2024-12-24T03:34:27Z |
| format | Article |
| id | doaj.art-425afec1db714311a3bb4fb044c2cea5 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-24T03:34:27Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-425afec1db714311a3bb4fb044c2cea52022-12-21T17:17:07ZengBMCBMC Genomics1471-21642017-04-0118111010.1186/s12864-017-3698-2Global analysis of protein lysine succinylation profiles in common wheatYumei Zhang0Guangyuan Wang1Limin Song2Ping Mu3Shu Wang4Wenxing Liang5Qi Lin6College of Agronomy and Plant Protection, Qingdao Agricultural UniversityCollege of Life Sciences, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural UniversityCollege of Agronomy and Plant Protection, Qingdao Agricultural UniversityCollege of Agronomy and Plant Protection, Qingdao Agricultural UniversityCollege of Agronomy, Shenyang Agricultural UniversityCollege of Agronomy and Plant Protection, Qingdao Agricultural UniversityCollege of Agronomy and Plant Protection, Qingdao Agricultural UniversityAbstract Background Protein lysine succinylation is an important post-translational modification and plays a critical regulatory role in almost every aspects of cell metabolism in both eukaryotes and prokaryotes. Common wheat is one of the major global cereal crops. However, to date, little is known about the functions of lysine succinylation in this plant. Here, we performed a global analysis of lysine succinylation in wheat and examined its overlap with lysine acetylation. Results In total, 330 lysine succinylated modification sites were identified in 173 proteins. Bioinformatics analysis showed that the modified proteins are distributed in multiple subcellular compartments and are involved in a wide variety of biological processes such as photosynthesis and the Calvin-Benson cycle, suggesting an important role for lysine succinylation in these processes. Five putative succinylation motifs were identified. A protein interaction network analysis revealed that diverse interactions are modulated by protein succinylation. Moreover, 21 succinyl-lysine sites were found to be acetylated at the same position, and 33 proteins were modified by both acetylation and succinylation, suggesting an extensive overlap between succinylation and acetylation in common wheat. Comparative analysis indicated that lysine succinylation is conserved between common wheat and Brachypodium distachyon. Conclusions These results suggest that lysine succinylation is involved in diverse biological processes, especially in photosynthesis and carbon fixation. This systematic analysis represents the first global analysis of lysine succinylation in common wheat and provides an important resource for exploring the physiological role of lysine succinylation in this cereal crop and likely in all plants.http://link.springer.com/article/10.1186/s12864-017-3698-2Lysine succinylationSuccinylomeTriticum aestivum L.Post-translational modificationPhotosynthesis |
| spellingShingle | Yumei Zhang Guangyuan Wang Limin Song Ping Mu Shu Wang Wenxing Liang Qi Lin Global analysis of protein lysine succinylation profiles in common wheat BMC Genomics Lysine succinylation Succinylome Triticum aestivum L. Post-translational modification Photosynthesis |
| title | Global analysis of protein lysine succinylation profiles in common wheat |
| title_full | Global analysis of protein lysine succinylation profiles in common wheat |
| title_fullStr | Global analysis of protein lysine succinylation profiles in common wheat |
| title_full_unstemmed | Global analysis of protein lysine succinylation profiles in common wheat |
| title_short | Global analysis of protein lysine succinylation profiles in common wheat |
| title_sort | global analysis of protein lysine succinylation profiles in common wheat |
| topic | Lysine succinylation Succinylome Triticum aestivum L. Post-translational modification Photosynthesis |
| url | http://link.springer.com/article/10.1186/s12864-017-3698-2 |
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