Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell line
<p>Abstract</p> <p>Background</p> <p><it>Piper sarmentosum</it>, locally known as kaduk is belonging to the family of Piperaceae. It is our interest to evaluate their effect on human hepatoma cell line (HepG2) for the potential of anticarcinogenic activity.&...
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| Format: | Article |
| Language: | English |
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BMC
2009-03-01
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| Series: | Cancer Cell International |
| Online Access: | http://www.cancerci.com/content/9/1/6 |
| _version_ | 1831726605484949504 |
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| author | Senafi Sahidan Safian Muhd Fauzi Zainal Ariffin Zaidah Wan Omar Wan Haifa Haryani Zainal Ariffin Shahrul Hisham Megat Abdul Wahab Rohaya |
| author_facet | Senafi Sahidan Safian Muhd Fauzi Zainal Ariffin Zaidah Wan Omar Wan Haifa Haryani Zainal Ariffin Shahrul Hisham Megat Abdul Wahab Rohaya |
| author_sort | Senafi Sahidan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Piper sarmentosum</it>, locally known as kaduk is belonging to the family of Piperaceae. It is our interest to evaluate their effect on human hepatoma cell line (HepG2) for the potential of anticarcinogenic activity.</p> <p>Results</p> <p>The anticarcinogenic activity of an ethanolic extract from <it>Piper sarmentosum </it>in HepG2 and non-malignant Chang's liver cell lines has been previously determined using (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) (MTT) assays, where the IC<sub>50 </sub>value was used as a parameter for cytotoxicity. The ethanolic extract that showed anticarcinogenic properties in HepG2 cells had an IC<sub>50 </sub>of 12.5 μg mL<sup>-1</sup>, while IC<sub>50 </sub>values in the non-malignant Chang's liver cell line were greater than 30 μg mL<sup>-1</sup>. Apoptotic morphological changes in HepG2 cells were observed using an inverted microscope and showed chromatin condensation, cell shrinkage and apoptotic bodies following May-Grunwald-Giemsa's staining. The percentage of apoptotic cells in the overall population (apoptotic index) showed a continuously significant increase (p < 0.05) in 12.5 μg mL<sup>-1 </sup>ethanolic extract-treated cells at 24, 48 and 72 hours compared to controls (untreated cells). Following acridine orange and ethidium bromide staining, treatment with 10, 12 and 14 μg mL<sup>-1 </sup>of ethanolic extracts caused typical apoptotic morphological changes in HepG2 cells. Molecular analysis of DNA fragmentation was used to examine intrinsic apoptosis induced by the ethanolic extracts. These results showed a typical intrinsic apoptotic characterisation, which included fragmentation of nuclear DNA in ethanolic extract-treated HepG2 cells. However, the non-malignant Chang's liver cell line produced no DNA fragmentation. In addition, the DNA genome was similarly intact for both the untreated non-malignant Chang's liver and HepG2 cell lines.</p> <p>Conclusion</p> <p>Therefore, our results suggest that the ethanolic extract from <it>P. sarmentosum </it>induced anticarcinogenic activity through an intrinsic apoptosis pathway in HepG2 cells <it>in vitro</it>.</p> |
| first_indexed | 2024-12-21T06:12:48Z |
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| id | doaj.art-425b5bc98c58427694a49dd4b3c8d38d |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-21T06:12:48Z |
| publishDate | 2009-03-01 |
| publisher | BMC |
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| series | Cancer Cell International |
| spelling | doaj.art-425b5bc98c58427694a49dd4b3c8d38d2022-12-21T19:13:29ZengBMCCancer Cell International1475-28672009-03-0191610.1186/1475-2867-9-6Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell lineSenafi SahidanSafian Muhd FauziZainal Ariffin ZaidahWan Omar Wan Haifa HaryaniZainal Ariffin Shahrul HishamMegat Abdul Wahab Rohaya<p>Abstract</p> <p>Background</p> <p><it>Piper sarmentosum</it>, locally known as kaduk is belonging to the family of Piperaceae. It is our interest to evaluate their effect on human hepatoma cell line (HepG2) for the potential of anticarcinogenic activity.</p> <p>Results</p> <p>The anticarcinogenic activity of an ethanolic extract from <it>Piper sarmentosum </it>in HepG2 and non-malignant Chang's liver cell lines has been previously determined using (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) (MTT) assays, where the IC<sub>50 </sub>value was used as a parameter for cytotoxicity. The ethanolic extract that showed anticarcinogenic properties in HepG2 cells had an IC<sub>50 </sub>of 12.5 μg mL<sup>-1</sup>, while IC<sub>50 </sub>values in the non-malignant Chang's liver cell line were greater than 30 μg mL<sup>-1</sup>. Apoptotic morphological changes in HepG2 cells were observed using an inverted microscope and showed chromatin condensation, cell shrinkage and apoptotic bodies following May-Grunwald-Giemsa's staining. The percentage of apoptotic cells in the overall population (apoptotic index) showed a continuously significant increase (p < 0.05) in 12.5 μg mL<sup>-1 </sup>ethanolic extract-treated cells at 24, 48 and 72 hours compared to controls (untreated cells). Following acridine orange and ethidium bromide staining, treatment with 10, 12 and 14 μg mL<sup>-1 </sup>of ethanolic extracts caused typical apoptotic morphological changes in HepG2 cells. Molecular analysis of DNA fragmentation was used to examine intrinsic apoptosis induced by the ethanolic extracts. These results showed a typical intrinsic apoptotic characterisation, which included fragmentation of nuclear DNA in ethanolic extract-treated HepG2 cells. However, the non-malignant Chang's liver cell line produced no DNA fragmentation. In addition, the DNA genome was similarly intact for both the untreated non-malignant Chang's liver and HepG2 cell lines.</p> <p>Conclusion</p> <p>Therefore, our results suggest that the ethanolic extract from <it>P. sarmentosum </it>induced anticarcinogenic activity through an intrinsic apoptosis pathway in HepG2 cells <it>in vitro</it>.</p>http://www.cancerci.com/content/9/1/6 |
| spellingShingle | Senafi Sahidan Safian Muhd Fauzi Zainal Ariffin Zaidah Wan Omar Wan Haifa Haryani Zainal Ariffin Shahrul Hisham Megat Abdul Wahab Rohaya Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell line Cancer Cell International |
| title | Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell line |
| title_full | Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell line |
| title_fullStr | Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell line |
| title_full_unstemmed | Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell line |
| title_short | Intrinsic anticarcinogenic effects of <it>Piper sarmentosum </it>ethanolic extract on a human hepatoma cell line |
| title_sort | intrinsic anticarcinogenic effects of it piper sarmentosum it ethanolic extract on a human hepatoma cell line |
| url | http://www.cancerci.com/content/9/1/6 |
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