Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin Inhibitors
Thymic stromal lymphopoietin (TSLP) plays an important role in the pathophysiology of various allergic diseases that are mediated by T helper cell type-2 (Th2) responses, including asthma and atopic dermatitis. The primary focus of this study was the identification of potent inhibitors of the TSLP s...
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MDPI AG
2021-08-01
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| author | Hyeji Shin Yoo Kyong Han Youngjoo Byun Young Ho Jeon Ki Yong Lee |
| author_facet | Hyeji Shin Yoo Kyong Han Youngjoo Byun Young Ho Jeon Ki Yong Lee |
| author_sort | Hyeji Shin |
| collection | DOAJ |
| description | Thymic stromal lymphopoietin (TSLP) plays an important role in the pathophysiology of various allergic diseases that are mediated by T helper cell type-2 (Th2) responses, including asthma and atopic dermatitis. The primary focus of this study was the identification of potent inhibitors of the TSLP signaling pathway for potential therapeutic use. The 80% methanol extract of <i>Machilus thunbergii</i> bark significantly inhibited the signal transducer and activator of transcription 5 (STAT5) phosphorylation in human mast cell (HMC)-1 cells. Through activity-guided isolation, three lignans (<b>1</b>–<b>3</b>) were obtained and identified as (+)-galbelgin (<b>1</b>), <i>meso</i>-dihydroguaiaretic acid (<b>2</b>), and machilin A (<b>3</b>). Among them, two lignans (<b>1</b> and <b>2</b>) significantly inhibited STAT5 phosphorylation and TSLP/TSLPR interaction, as determined by ELISA. Our results indicated that lignans isolated from <i>M. thunbergii</i> are a promising resource for the treatment of allergic diseases. |
| first_indexed | 2024-03-10T08:33:02Z |
| format | Article |
| id | doaj.art-425baf74cbe045f487facf1f79020044 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:33:02Z |
| publishDate | 2021-08-01 |
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| series | Molecules |
| spelling | doaj.art-425baf74cbe045f487facf1f790200442023-11-22T08:52:13ZengMDPI AGMolecules1420-30492021-08-012616480410.3390/molecules26164804Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin InhibitorsHyeji Shin0Yoo Kyong Han1Youngjoo Byun2Young Ho Jeon3Ki Yong Lee4College of Pharmacy, Korea University, Sejong 30019, KoreaCollege of Pharmacy, Korea University, Sejong 30019, KoreaCollege of Pharmacy, Korea University, Sejong 30019, KoreaCollege of Pharmacy, Korea University, Sejong 30019, KoreaCollege of Pharmacy, Korea University, Sejong 30019, KoreaThymic stromal lymphopoietin (TSLP) plays an important role in the pathophysiology of various allergic diseases that are mediated by T helper cell type-2 (Th2) responses, including asthma and atopic dermatitis. The primary focus of this study was the identification of potent inhibitors of the TSLP signaling pathway for potential therapeutic use. The 80% methanol extract of <i>Machilus thunbergii</i> bark significantly inhibited the signal transducer and activator of transcription 5 (STAT5) phosphorylation in human mast cell (HMC)-1 cells. Through activity-guided isolation, three lignans (<b>1</b>–<b>3</b>) were obtained and identified as (+)-galbelgin (<b>1</b>), <i>meso</i>-dihydroguaiaretic acid (<b>2</b>), and machilin A (<b>3</b>). Among them, two lignans (<b>1</b> and <b>2</b>) significantly inhibited STAT5 phosphorylation and TSLP/TSLPR interaction, as determined by ELISA. Our results indicated that lignans isolated from <i>M. thunbergii</i> are a promising resource for the treatment of allergic diseases.https://www.mdpi.com/1420-3049/26/16/4804<i>Machilus thunbergii</i>TSLPSTAT5allergic diseaselignan |
| spellingShingle | Hyeji Shin Yoo Kyong Han Youngjoo Byun Young Ho Jeon Ki Yong Lee Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin Inhibitors Molecules <i>Machilus thunbergii</i> TSLP STAT5 allergic disease lignan |
| title | Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin Inhibitors |
| title_full | Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin Inhibitors |
| title_fullStr | Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin Inhibitors |
| title_full_unstemmed | Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin Inhibitors |
| title_short | Lignans from <i>Machilus thunbergii</i> as Thymic Stromal Lymphopoietin Inhibitors |
| title_sort | lignans from i machilus thunbergii i as thymic stromal lymphopoietin inhibitors |
| topic | <i>Machilus thunbergii</i> TSLP STAT5 allergic disease lignan |
| url | https://www.mdpi.com/1420-3049/26/16/4804 |
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