Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens
Abstract Field emission scanning electron microscopy (FESEM) is an essential tool for observing surface details of specimens in a high vacuum. A series of specimen procedures precludes the observations of living organisms, resulting in artifacts. To overcome these problems, Takahiko Hariyama and his...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-05-01
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| Series: | Applied Microscopy |
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| Online Access: | https://doi.org/10.1186/s42649-022-00073-2 |
| _version_ | 1818201929007759360 |
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| author | Ki Woo Kim |
| author_facet | Ki Woo Kim |
| author_sort | Ki Woo Kim |
| collection | DOAJ |
| description | Abstract Field emission scanning electron microscopy (FESEM) is an essential tool for observing surface details of specimens in a high vacuum. A series of specimen procedures precludes the observations of living organisms, resulting in artifacts. To overcome these problems, Takahiko Hariyama and his colleagues proposed the concept of the “nanosuit” later referred to as “NanoSuit”, describing a thin polymer layer placed on organisms to protect them in a high vacuum in 2013. The NanoSuit is formed rapidly by (i) electron beam irradiation, (ii) plasma irradiation, (iii) Tween 20 solution immersion, and (iv) surface shield enhancer (SSE) solution immersion. Without chemical fixation and metal coating, the NanoSuit-formed specimens allowed structural preservation and accurate element detection of insulating, wet specimens at high spatial resolution. NanoSuit-formed larvae were able to resume normal growth following FESEM observation. The method has been employed to observe unfixed and uncoated bacteria, multicellular organisms, and paraffin sections. These results suggest that the NanoSuit can be applied to prolong life in vacuo and overcome the limit of dead imaging of electron microscopy. |
| first_indexed | 2024-12-12T03:01:21Z |
| format | Article |
| id | doaj.art-425dd37ba98a4aa6b631c7923d14cca5 |
| institution | Directory Open Access Journal |
| issn | 2287-4445 |
| language | English |
| last_indexed | 2024-12-12T03:01:21Z |
| publishDate | 2022-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Microscopy |
| spelling | doaj.art-425dd37ba98a4aa6b631c7923d14cca52022-12-22T00:40:38ZengSpringerOpenApplied Microscopy2287-44452022-05-0152111110.1186/s42649-022-00073-2Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimensKi Woo Kim0Department of Ecology and Environmental System, Kyungpook National UniversityAbstract Field emission scanning electron microscopy (FESEM) is an essential tool for observing surface details of specimens in a high vacuum. A series of specimen procedures precludes the observations of living organisms, resulting in artifacts. To overcome these problems, Takahiko Hariyama and his colleagues proposed the concept of the “nanosuit” later referred to as “NanoSuit”, describing a thin polymer layer placed on organisms to protect them in a high vacuum in 2013. The NanoSuit is formed rapidly by (i) electron beam irradiation, (ii) plasma irradiation, (iii) Tween 20 solution immersion, and (iv) surface shield enhancer (SSE) solution immersion. Without chemical fixation and metal coating, the NanoSuit-formed specimens allowed structural preservation and accurate element detection of insulating, wet specimens at high spatial resolution. NanoSuit-formed larvae were able to resume normal growth following FESEM observation. The method has been employed to observe unfixed and uncoated bacteria, multicellular organisms, and paraffin sections. These results suggest that the NanoSuit can be applied to prolong life in vacuo and overcome the limit of dead imaging of electron microscopy.https://doi.org/10.1186/s42649-022-00073-2NanoSuitScanning electron microscopyVacuum |
| spellingShingle | Ki Woo Kim Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens Applied Microscopy NanoSuit Scanning electron microscopy Vacuum |
| title | Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens |
| title_full | Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens |
| title_fullStr | Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens |
| title_full_unstemmed | Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens |
| title_short | Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens |
| title_sort | biological applications of the nanosuit for electron imaging and x microanalysis of insulating specimens |
| topic | NanoSuit Scanning electron microscopy Vacuum |
| url | https://doi.org/10.1186/s42649-022-00073-2 |
| work_keys_str_mv | AT kiwookim biologicalapplicationsofthenanosuitforelectronimagingandxmicroanalysisofinsulatingspecimens |